RESUMEN

Mycenon (C11H5Cl3O3), a new inhibitor of isocitrate lyase (EC 4.1.3.1) was isolated from the culture broth of a basidiomycete, Mycena sp. Mycenon is a novel chlorinated benzoquinone derivative which is also active against bacteria and fungi. Malate synthase (EC 4.1.3.2) the second key enzyme of the glyoxylate cycle was not affected by mycenon. Isocitrate lyase preparations from plants, bacteria and fungi were sensitive. The following Ki-values for mycenon have been determined: Ricinus communis, 5.2 microM; Acinetobacter calcoaceticus, 11 microM; Neurospora crassa, 7.4 microM. The structure of mycenon has been determined by a single crystal X-ray analysis.

Asunto(s)

Agaricales/metabolismo , Benzoquinonas/química , Isocitratoliasa/antagonistas & inhibidores , Animales , Bacterias/efectos de los fármacos , Bacterias/enzimología , Benzoquinonas/aislamiento & purificación , Benzoquinonas/farmacología , Carcinoma de Ehrlich , Fermentación , Hongos/efectos de los fármacos , Hongos/enzimología , Células HeLa , Humanos , Cinética , Leucemia L1210 , Estructura Molecular , Plantas/enzimología , Solubilidad , Células Tumorales Cultivadas , Difracción de Rayos X