RESUMEN
A type of biodegradable microsphere (DSM), approximately 45 microns in diameter, made of polymerized potato starch (Pharmacia, Sweden) was intravenously injected into rats to observe the state of DSM in small blood vessels in the kidney and liver at the electron microscopic level. Prior to their digestion with amylase, individual DSM changed their round shape to an irregularly folded one to occupy almost the whole area of the lumen. At the transmission electron microscopic level, DSM were impregnated with colloidal iron and were easily identified. Interaction of the iron labelled DSM with the surface of endothelial cells was unexpectedly loose and no adherence or fusion of this surface was observed. The starch substance was not visible in the pinocytotic vesicles of the endothelium. These findings suggest the independent profile of DSM in situ.
Asunto(s)
Vasos Sanguíneos/ultraestructura , Cápsulas , Animales , Biodegradación Ambiental , Microscopía Electrónica , Microscopía Electrónica de Rastreo , Microesferas , Ratas , Almidón/metabolismoAsunto(s)
Trastornos de la Coagulación Sanguínea/tratamiento farmacológico , Colágeno/uso terapéutico , Técnicas Hemostáticas , Hemostáticos/uso terapéutico , Animales , Aspirina/toxicidad , Trastornos de la Coagulación Sanguínea/etiología , Bovinos , Perros , Hemorragia/tratamiento farmacológico , Heparina/toxicidad , Bazo/lesiones , Tiofenos/toxicidad , Trombocitopenia/complicaciones , Ticlopidina , Warfarina/toxicidadRESUMEN
Intraduodenally administered 14C-urokinase (14C-UK) was located in Macaca Irus by autoradiography. Sagittal sections, approximately 40 micron thick, of a frozen monkey were applied against X-ray films. Autoradiograms thus obtained indicated that a part of intraduodenally administered 14C-UK was incorporated into the systemic blood circulation by transintestinal mucosal membrane.
Asunto(s)
Absorción Intestinal , Activador de Plasminógeno de Tipo Uroquinasa/metabolismo , Animales , Autorradiografía , Radioisótopos de Carbono , Duodeno/metabolismo , Macaca , MasculinoRESUMEN
Secretion granules in the shell gland, isthmus, and albumin-secreting region of the hen oviduct were analyzed with WET-scanning electron microscopy (SEM) and EDX, a combination of wide-angle backscattered electron detector (BED) and energy-dispersive X-ray microanalyzer (EDX). Glutaraldehyde-fixed but unhydrated, unstained, and uncoated samples were analyzed; Ca was localized in all secretion granules in all three sections of the hen oviduct studied.
Asunto(s)
Calcio/análisis , Gránulos Citoplasmáticos/análisis , Oviductos/análisis , Animales , Pollos , Microanálisis por Sonda Electrónica , Femenino , Técnicas In Vitro , Microscopía Electrónica de Rastreo , Oviductos/ultraestructuraRESUMEN
In order to evaluate the effect of urokinase on thrombus a model of experimental thrombus was formed at left lateral saphena vein of beagle dogs by an injection of canine or bovine fibrinogen and bovine thrombin. Surface and cut-surface of thus formed thrombus were observed with a field emission Scanning Electron Microscope (SEM). Despite considerable variations among dogs the thrombus decreased its volume and compactness as time passed. The proportion of deformed erythrocytes and non-erythrocyte components of thrombus increased at 6, 15 and 24 hours after thrombogenesis. Erythrocytes penetrate into spaces between fibrin sheets and covered with fibrin network. The fibrin fibrils became thick in later stages. The surface of endothelium did not alter extensively by the presence of thrombus. Natural thrombolysis occurred between 15 and 24 hours after thrombogenesis. Recannalization of the vein was monitored with X-ray angiogram and the measurement of FDP.
Asunto(s)
Vena Safena/ultraestructura , Trombosis/patología , Animales , Modelos Animales de Enfermedad , Perros , Endotelio/ultraestructura , Eritrocitos/ultraestructura , Fibrina/análisis , Masculino , Microscopía Electrónica de Rastreo/métodos , Vena Safena/patologíaRESUMEN
A spontaneous tumor was obtained from a hen of White-Leghorn stock raised in a local farm in Yamaguchi City, in December 1973. The tumor had characteristics of fibro- or myxofibro-sarcoma and could be maintained by transplantation in chickens of similar flocks. This tumor was proved to produce sarcoma virus at 33rd passage and the transforming virus was designated as Y73 sarcoma virus. Biological studies suggested that the virus recovered from the tumor belonged to avian leukosis-sarcoma complex having subgroup A specificity. The possibility of defectiveness of this virus will be discussed.