RESUMEN
Cardiovascular disorders, including acute myocardial infarction (AMI), often lead to blood clot formation, impacting blood circulation. Streptokinase, a cost-effective and widely available thrombolytic agent, is crucial in treating thrombosis. This study aimed to produce streptokinase from Streptococcus pyogenes EBL-48 and compare its efficacy with heparin in an animal model. We evaluated the clot-lysing effectiveness of streptokinase produced from Streptococcus pyogenes EBL-48, emphasizing its low cost and ease of production. Streptokinase was produced using pre-optimized fermentation media and purified through ion exchange and gel-filtration chromatography. In vivo analysis involved inducing clots in a trial animal model using ferric chloride, comparing streptokinase with heparin. Ultrasonography assessed the clot-lysing activity of streptokinase. Streptokinase (47 kDa) effectively lysed clots, proving its low cost, easy production, and minimal adverse effects. Ultrasonography confirmed its fibrinolytic efficacy. These findings highlight potential as an affordable and easily produced thrombolytic agent, particularly relevant in resource-limited settings. Streptokinase efficacy and minimal adverse effects make it a promising option for thrombolytic therapy, especially in economically constrained regions. Future studies could optimize production techniques, explore different strains, and conduct clinical trials for human validation. Comparative studies with other thrombolytic agents would enhance understanding of their advantages and limitations.
Asunto(s)
Fibrinolíticos , Estreptoquinasa , Animales , Fermentación , Fibrinolíticos/farmacología , Fibrinolíticos/uso terapéutico , Heparina , Estreptoquinasa/farmacología , Estreptoquinasa/uso terapéutico , Resultado del TratamientoRESUMEN
We propose herein initial results to develop optimum redox mediators by the combination of computational simulation and catalytic functionalization of the core structure of vitamin K3. We aim to correlate the calculated energy value of the LUMO of different vitamin K3 derivatives with their actual redox potential. For this, we optimized the catalytic alkylation of 1,4-naphthoquinones with a designed Ag(i)/GO catalyst and synthesized a series of molecules.
RESUMEN
An aqueous extract of Cynomorium coccineum was administered by stomach tube to ten mature male Wistar rats, at a dose of 47 mg/100 kg body weight/day for 14 consecutive days. Ten rats were kept as controls and received normal saline by oral route at the same dosing interval. Sperm was collected from the epididymes after decapitation. The results revealed that the water extract of the Cynomorium coccineum induced significant increase in the sperm count, improved the percentage of live sperm and their motility and decreased the number of abnormal sperm. Testicular histology showed increased spermatogenesis and seminiferous tubules full of sperm in the treated group compared with the controls.