RESUMEN
The genetics of T lymphocyte/accessory cell interactions were studied by using antigen-specific monoclonal T cell lines from human peripheral blood and X-irradiated non-T cells (E rosette-depleted) as accessory cells. The T cell lines were obtained by expanding single colonies from soft agar-cloned E rosette-positive lymphocytes, previously incubated for 3 days in liquid culture with 30% autologous X-irradiated non-T cells and antigen (PPD). When investigated with both autologous and allogeneic accessory cells of various HLA-D genotype, different sets of PPD-specific T cell lines were obtained from HLA-D heterozygous individuals; two sets restricted to each one of the two HLA-D antigens, and a third set responding to PPD only when presented by accessory cells carrying both HLA-D antigens possessed by the T cell donor. A few cell lines responded to PPD only in the presence of autologous accessory cells.
Asunto(s)
Antígenos de Histocompatibilidad Clase II/genética , Linfocitos T/inmunología , Células Clonales/inmunología , Epítopos , Heterocigoto , Antígenos de Histocompatibilidad Clase II/inmunología , Humanos , Activación de Linfocitos , Prueba de Cultivo Mixto de Linfocitos , Timidina/metabolismo , Tuberculina/inmunologíaRESUMEN
The effect of D-penicillamine (D-Pen) on the proliferation of cultures of normal mouse, rat, and human spleen lymphocytes and peripheral blood lymphocytes was examined. D-Pen in concentrations of 2 x 10(-3) M to 8 x 10(-3) M in serum-free and in serum-containing medium resulted in a highly significant incorporation of 3H-TdR by normal mouse and rat spleen cells. Enhanced incorporation of 3H-TdR by normal human spleen cells only occurred in serum-containing medium. D-Pen in concentrations of 10(-4) M to 10(-3) M in serum-free and serum-containing medium resulted in significant inhibition of 3H-TdR incorporation by normal and mitogen-stimulated mouse and rat spleen cells. Doses of D-Pen greater than 2 x 10(-2) M strongly inhibited 3H-TdR incorporation by both normal and mitogen-stimulated mouse, rat, and human spleen cells and peripheral blood cells. The latter cells were not stimulated or inhibited at lower concentrations of D-Pen. Results from cell depletion and enriching procedures (specific antibody +C' cell killing, employment of athymic, nude spleen cells, adherent and phagocytic cell removal, E rosette cell separation procedures) suggested that target cells in the mouse spleen for D-Pen activation are non-adherent B cells whereas the D-Pen responsive cells in the human spleen probably are T cells.
Asunto(s)
Linfocitos B/efectos de los fármacos , Activación de Linfocitos , Penicilamina/farmacología , Linfocitos T/efectos de los fármacos , Animales , Linfocitos B/metabolismo , Sangre , ADN/biosíntesis , Relación Dosis-Respuesta a Droga , Humanos , Ratones , Fitohemaglutininas/farmacología , Ratas , Linfocitos T/metabolismoRESUMEN
The in vitro and in vivo effects of therapeutical doses of acetylsalicylic acid on lymphocyte subpopulations in peripheral blood were investigated with the following results: Acetylsalicylic acid caused both in vitro and in vivo a reduction of complement receptor bearing lymphocytes and of lymphocytes identified with fluorescent rabbit antibody to human Ig (polyvalent) and to human IgG. Sheep red blood cell receptor bearing lymphocytes, and lymphocytes identified with antibody to human IgM and IgD were unaffected by acetylsalicylic acid.
Asunto(s)
Aspirina/farmacología , Linfocitos/efectos de los fármacos , Proteínas del Sistema Complemento , Técnica del Anticuerpo Fluorescente , Humanos , Fragmentos Fc de Inmunoglobulinas , Inmunoglobulina G , Inmunoglobulina M , Recuento de Leucocitos , Linfocitos/inmunología , Formación de Roseta , Coloración y Etiquetado , Linfocitos T/efectos de los fármacos , Linfocitos T/inmunologíaRESUMEN
Twenty patients with contact dermatitis, eighteen with atopic dermatitis, two with mixed dermatitis, and 20 controls were examined for the number of T and B lymphocytes, and serum concentrations of IgD and IgE. Significantly higher counts of B lymphocytes bearing IgE and high serum IgE values were found in the atopic group. No other significant differences were found. In particular, we found a normal frequency of IgD bearing lymphocytes in contact dermatitis, and normal T lymphocyte counts in both groups of patients.
Asunto(s)
Linfocitos B/inmunología , Dermatitis Atópica/inmunología , Dermatitis por Contacto/inmunología , Linfocitos T/inmunología , Adolescente , Adulto , Anciano , Niño , Femenino , Humanos , Inmunoglobulina D/análisis , Inmunoglobulina E/análisis , Recuento de Leucocitos , Masculino , Persona de Mediana EdadRESUMEN
Specific HLA antibodies were used to eliminate donor and recipient cells, respectively, from lymphocyte suspensions prepared from the blood of a child who had been transplanted with bone marrow from an HLA-A- and HLA-B-incompatible, HLA-D-compatible donor. About 70% of the lymphocytes were of donor HLA type, the remaining of recipient type. The phytohemagglutinin-responsive lymphocytes were exclusively limited to the lymphocyte population carrying donor-type HLA antigens. Membrane immunofluorescence investigations of the donor and recipient populations showed a low percentage of IgM-positive lymphocytes in the donor population and an extremely high proportion of IgM-positive lymphocytes in the recipient population. About 90% of the donor lymphocytes were T cells, as judged by their capacity to form rosettes between sheep erythrocytes and T lymphocytes; no cells in the recipient cell population expressed this ability.
Asunto(s)
Células de la Médula Ósea , Trasplante de Médula Ósea , Síndromes de Inmunodeficiencia/terapia , Linfocitos/inmunología , Linfocitos B/inmunología , Supervivencia Celular , Células Cultivadas , Niño , Proteínas del Sistema Complemento , Antígenos HLA , Prueba de Histocompatibilidad , Humanos , Reacción de Inmunoadherencia , Activación de Linfocitos , Masculino , Receptores de Antígenos de Linfocitos B/análisis , Linfocitos T/inmunología , Trasplante HomólogoRESUMEN
The genetic control of strong stimulation in the mixed lymphocyte culture reaction is determined by a separate gene (MLR-S) closely linked to the FOUR-locus of the HL-A chromosomal region. Three additional examples of siblings with recombination between FOUR-locus and MLR-S locus are presented which confirms the independent genetic control of mixed lymphocyte reaction from the control of HL-A antigens. The occurrence of two recombinant children in one family with four other children representing all possible HL-A haplotype-combinations, strongly supports the genetic mapping of the MLR-S determinants outside the HL-A chromosomal region. The experiments presented show that additional genes located within the HL-A region itself contribute with a weak stimulation of allogenic mixtures. These data are discussed in relation to the marginal stimulation of the mixed lymphocyte culture reaction which can be seen between unrelated individuals. It seems that a group of relatively histocompatible individuals can be defined by identity of the MLR-S locus but with differences on the weak MLC-determinants, and that this group for the purpose of clinical transplantation behaves as histocompatible individuals.