RESUMEN
PURPOSE: To study the feasibility of tissue engineered bone constructed with simvastatin carried by PLGA/CPC and bone marrow stromal cells (BMSCs) and screen the effective drug loading of simvastatin. METHODS: Solvent casting-particle leaching technology combined with the phase separation process was used to prepare the different concentrations (simvastatin mass: 0.1, 0.5, 1 mg) of simvastatin carried by PLGA/CPC composite scaffold materials. Scanning electron microscopy was used to observe the porosity and drug release curve was drawn; Alizarin red staining and type I collagen staining were applied to observe the effect of osteogenic medium and simvastatin on the role of BMSCs to the osteogenetic differentiation. The induced passage 3 cells after dil staining were mixed with the composite scaffold material to a complex. Scanning electron microscopy and laser confocal microscope were used to observe the adhesion on the complex. CCK-8 and alkaline phosphatase (ALP) were applied to observe the proliferation and differentiation. SPSS 18.0 software package was used for statistical analysis. RESULTS: The scaffold porosity was more than 90% with an average aperture of 200-300 µm. The drug released slowly. There was no obvious interpretation. Type I collagen showed positive expression. Alizarin red staining proofed the formation of mineralization nodules in group which was induced with the conditional medium and simvastatin. 0.5 mg group showed cells adhered to the inner surface of the scaffold material and could significantly promote the proliferation and differentiation of cells. CONCLUSIONS: Combination of simvastatin and osteogenic medium can effectively promote the differentiation of BMSCs. Simvastatin carried by PLGA/CPC scaffold materials is an ideal tissue engineering scaffold material. PLGA/CPC scaffold containing 0.5 mg simvastatin can effectively promote the proliferation and differentiation of BMSCs. Supported by Natural Science Foundation of Jilin Province (201215052).
Asunto(s)
Células Madre Mesenquimatosas , Simvastatina , Fosfatasa Alcalina , Células de la Médula Ósea , Fosfatos de Calcio , Diferenciación Celular , Ácido Láctico , Osteogénesis , Ácido Poliglicólico/análogos & derivados , Copolímero de Ácido Poliláctico-Ácido Poliglicólico , Ingeniería de Tejidos , Andamios del TejidoRESUMEN
L-proline chiral stationary phases with various bonding amounts for ligand exchange chromatography were prepared by treating silica gel with 3-glycidoxypropyltrimethoxysilane and bonding L-proline. The bonding amounts of L-proline chiral stationary phase were controlled effectively and characterized by IR and elemental analysis. Some DL-amino acids were separated and the influences of the bonding amounts on the efficiency of resolution of DL-amino acids by ligand exchange chromatography were briefly discussed and explained by the mechanism of the separation.
Asunto(s)
Aminoácidos/aislamiento & purificación , Cromatografía Líquida de Alta Presión/instrumentación , Aminoácidos/química , Cromatografía Líquida de Alta Presión/métodos , Dicroismo Circular , Enlace de Hidrógeno , Ligandos , Prolina/aislamiento & purificación , Espectrofotometría Infrarroja , Estereoisomerismo , Tirosina/aislamiento & purificaciónRESUMEN
The chiral separation of some chiral compounds with similar structure on the cellulose tris (3,5-dimethylphenylcarbamate) chiral stationary phase prepared by us was obtained. Ternary mobile phases influencing chiral recognition were investigated. A mode of interaction between the structural character of samples and chiral stationary phase is discussed. The results indicated that the retention and chiral separation of the analytes had a bigger change with minute addition of alcohols or acetonitrile as modifier in n-hexane/2-propanol (80/20, volume ratio) binary mobile phase.