1.
Nat Methods
; 8(7): 571-3, 2011 Jun 05.
Artículo
en Inglés
| MEDLINE
| ID: mdl-21642963
RESUMEN
Applying pulsed excitation together with time-gated detection improves the fluorescence on-off contrast in continuous-wave stimulated emission depletion (CW-STED) microscopy, thus revealing finer details in fixed and living cells using moderate light intensities. This method also enables super-resolution fluorescence correlation spectroscopy with CW-STED beams, as demonstrated by quantifying the dynamics of labeled lipid molecules in the plasma membrane of living cells.