RESUMEN
We report on the specificity of a monoclonal antibody which reacts with autoclaved extracts of four species of enterococci but does not react to the same extent with similar extracts from two non-enterococcal group D streptococci. The monoclonal antibody also reacts specifically with purified lipoteichoic acid from Streptococcus faecalis but not significantly with purified lipoteichoic acid from the non-enterococcal species Streptococcus bovis and Streptococcus equinus. The specific antigen detected with this antibody could correlate with the definition of the enterococcus subgroup of the streptococci which would provide further evidence that this sub-group is taxonomically distinct from the other group D streptococci.
Asunto(s)
Anticuerpos Monoclonales , Enterococcus/inmunología , Streptococcus/inmunología , Anticuerpos Antibacterianos , Especificidad de Anticuerpos , Antígenos Bacterianos , Enterococcus/clasificación , Ensayo de Inmunoadsorción Enzimática , Lipopolisacáridos/inmunología , Especificidad de la Especie , Streptococcus/clasificación , Ácidos Teicoicos/inmunologíaRESUMEN
A rapid method for determining the sensitivity of Staphylococcus aureus to beta-lactams is described. The method involves the culture of microcolonies of the bacterium on the surface of antibiotic-containing agar medium on microscope slides. The areas of the microcolonies formed are measured by means of a microscope and image analyzer. The sensitivity of the bacterium can be estimated in 4 h by this technique and the results are comparable to those obtained by overnight incubation.