RESUMEN
Some bacteriophages target potentially pathogenic bacteria by exploiting surface-associated virulence factors as receptors. For example, phage have been identified that exhibit specificity for Vi capsule producing Salmonella enterica serovar Typhi. Here we have characterized the Vi-associated E1-typing bacteriophage using a number of molecular approaches. The absolute requirement for Vi capsule expression for infectivity was demonstrated using different Vi-negative S. enterica derivatives. The phage particles were shown to have an icosahedral head and a long noncontractile tail structure. The genome is 45,362 bp in length with defined capsid and tail regions that exhibit significant homology to the S. enterica transducing phage ES18. Mass spectrometry was used to confirm the presence of a number of hypothetical proteins in the Vi phage E1 particle and demonstrate that a number of phage proteins are modified posttranslationally. The genome of the Vi phage E1 is significantly related to other bacteriophages belonging to the same serovar Typhi phage-typing set, and we demonstrate a role for phage DNA modification in determining host specificity.
Asunto(s)
ADN Viral/genética , Fagos de Salmonella/genética , Salmonella typhi/virología , Clonación Molecular , ADN Viral/química , Genoma Viral , Espectrometría de Masas , Microscopía Electrónica de Transmisión , Datos de Secuencia Molecular , Fagos de Salmonella/crecimiento & desarrollo , Fagos de Salmonella/ultraestructura , Análisis de Secuencia de ADNRESUMEN
Comparisons of the 1.84-Mb genome of serotype M5 Streptococcus pyogenes strain Manfredo with previously sequenced genomes emphasized the role of prophages in diversification of S. pyogenes and the close relationship between strain Manfredo and MGAS8232, another acute rheumatic fever-associated strain.
Asunto(s)
Genoma Bacteriano , Fiebre Reumática/microbiología , Streptococcus pyogenes/genética , Streptococcus pyogenes/metabolismo , Perfilación de la Expresión Génica , Regulación Bacteriana de la Expresión Génica/fisiología , Variación Genética , Datos de Secuencia Molecular , FilogeniaRESUMEN
The genus Coccolithovirus is a recently discovered group of viruses that infect the globally important marine calcifying microalga Emiliania huxleyi. Among the 472 predicted genes of the 407,339-base pair genome are a variety of unexpected genes, most notably those involved in biosynthesis of ceramide, a sphingolipid known to induce apoptosis. Uniquely for algal viruses, it also contains six RNA polymerase subunits and a novel promoter, suggesting this virus encodes its own transcription machinery. Microarray transcriptomic analysis reveals that 65% of the predicted virus-encoded genes are expressed during lytic infection of E. huxleyi.
Asunto(s)
Genoma Viral , Phycodnaviridae/genética , Phycodnaviridae/fisiología , Análisis de Secuencia de ADN , Transcripción Genética , Apoptosis , Composición de Base , Ceramidas/biosíntesis , Biología Computacional , ADN Viral/química , ADN Viral/genética , ARN Polimerasas Dirigidas por ADN/genética , Eucariontes/virología , Expresión Génica , Perfilación de la Expresión Génica , Genes Virales , Análisis de Secuencia por Matrices de Oligonucleótidos , Péptido Hidrolasas/genética , Phycodnaviridae/clasificación , Regiones Promotoras Genéticas , Subunidades de Proteína , Esfingolípidos/biosíntesis , Replicación ViralRESUMEN
African trypanosomes cause human sleeping sickness and livestock trypanosomiasis in sub-Saharan Africa. We present the sequence and analysis of the 11 megabase-sized chromosomes of Trypanosoma brucei. The 26-megabase genome contains 9068 predicted genes, including approximately 900 pseudogenes and approximately 1700 T. brucei-specific genes. Large subtelomeric arrays contain an archive of 806 variant surface glycoprotein (VSG) genes used by the parasite to evade the mammalian immune system. Most VSG genes are pseudogenes, which may be used to generate expressed mosaic genes by ectopic recombination. Comparisons of the cytoskeleton and endocytic trafficking systems with those of humans and other eukaryotic organisms reveal major differences. A comparison of metabolic pathways encoded by the genomes of T. brucei, T. cruzi, and Leishmania major reveals the least overall metabolic capability in T. brucei and the greatest in L. major. Horizontal transfer of genes of bacterial origin has contributed to some of the metabolic differences in these parasites, and a number of novel potential drug targets have been identified.
Asunto(s)
Genoma de Protozoos , Glutatión/análogos & derivados , Proteínas Protozoarias/genética , Análisis de Secuencia de ADN , Espermidina/análogos & derivados , Trypanosoma brucei brucei/genética , Aminoácidos/metabolismo , Animales , Variación Antigénica , Antígenos de Protozoos/química , Antígenos de Protozoos/genética , Antígenos de Protozoos/inmunología , Metabolismo de los Hidratos de Carbono , Cromosomas/genética , Citoesqueleto/química , Citoesqueleto/genética , Citoesqueleto/fisiología , Ergosterol/biosíntesis , Genes Protozoarios , Glutatión/metabolismo , Glicosilfosfatidilinositoles/biosíntesis , Humanos , Metabolismo de los Lípidos , Datos de Secuencia Molecular , Transporte de Proteínas , Proteínas Protozoarias/química , Proteínas Protozoarias/metabolismo , Seudogenes , Purinas/metabolismo , Pirimidinas/biosíntesis , Recombinación Genética , Espermidina/metabolismo , Trypanosoma brucei brucei/química , Trypanosoma brucei brucei/inmunología , Trypanosoma brucei brucei/metabolismo , Tripanosomiasis Africana/parasitologíaRESUMEN
The oomycete Phytophthora infestans causes late blight, the potato disease that precipitated the Irish famines in 1846 and 1847. It represents a reemerging threat to potato production and is one of >70 species that are arguably the most devastating pathogens of dicotyledonous plants. Nevertheless, little is known about the molecular bases of pathogenicity in these algae-like organisms or of avirulence molecules that are perceived by host defenses. Disease resistance alleles, products of which recognize corresponding avirulence molecules in the pathogen, have been introgressed into the cultivated potato from a wild species, Solanum demissum, and R1 and R3a have been identified. We used association genetics to identify Avr3a and show that it encodes a protein that is recognized in the host cytoplasm, where it triggers R3a-dependent cell death. Avr3a resides in a region of the P. infestans genome that is colinear with the locus containing avirulence gene ATR1(NdWsB) in Hyaloperonospora parasitica, an oomycete pathogen of Arabidopsis. Remarkably, distances between conserved genes in these avirulence loci were often similar, despite intervening genomic variation. We suggest that Avr3a has undergone gene duplication and that an allele evading recognition by R3a arose under positive selection.
Asunto(s)
Proteínas Algáceas/genética , Apoptosis/genética , Phytophthora/genética , Phytophthora/patogenicidad , Solanum tuberosum/microbiología , Agrobacterium tumefaciens , Proteínas Algáceas/metabolismo , Secuencia de Aminoácidos , Secuencia de Bases , Biolística , Cromosomas Artificiales Bacterianos , Citoplasma/metabolismo , Cartilla de ADN , Duplicación de Gen , Vectores Genéticos , Proteínas Fluorescentes Verdes , Funciones de Verosimilitud , Modelos Genéticos , Datos de Secuencia Molecular , Filogenia , Potexvirus , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Alineación de Secuencia , Análisis de Secuencia de ADN , Solanum tuberosum/genética , Sintenía/genética , VirulenciaRESUMEN
The Salmonella enterica serovar Typhi CT18 (S.Typhi) chromosome harbours seven distinct prophage-like elements, some of which may encode functional bacteriophages. In silico analyses were used to investigate these regions in S.Typhi CT18, and ultimately compare these integrated bacteriophages against 40 other Salmonella isolates using DNA microarray technology. S.Typhi CT18 contains prophages that show similarity to the lambda, Mu, P2 and P4 bacteriophage families. When compared to other S.Typhi isolates, these elements were generally conserved, supporting a clonal origin of this serovar. However, distinct variation was detected within a broad range of Salmonella serovars; many of the prophage regions are predicted to be specific to S.Typhi. Some of the P2 family prophage analysed have the potential to carry non-essential "cargo" genes within the hyper-variable tail region, an observation that suggests that these bacteriophage may confer a level of specialisation on their host. Lysogenic bacteriophages therefore play a crucial role in the generation of genetic diversity within S.enterica.
Asunto(s)
Profagos/química , Fagos de Salmonella/química , Salmonella enterica/virología , Secuencia de Aminoácidos , Secuencia de Bases , Cartilla de ADN , Genoma Bacteriano , Datos de Secuencia Molecular , Salmonella enterica/genética , Homología de Secuencia de AminoácidoRESUMEN
Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica are closely related Gram-negative beta-proteobacteria that colonize the respiratory tracts of mammals. B. pertussis is a strict human pathogen of recent evolutionary origin and is the primary etiologic agent of whooping cough. B. parapertussis can also cause whooping cough, and B. bronchiseptica causes chronic respiratory infections in a wide range of animals. We sequenced the genomes of B. bronchiseptica RB50 (5,338,400 bp; 5,007 predicted genes), B. parapertussis 12822 (4,773,551 bp; 4,404 genes) and B. pertussis Tohama I (4,086,186 bp; 3,816 genes). Our analysis indicates that B. parapertussis and B. pertussis are independent derivatives of B. bronchiseptica-like ancestors. During the evolution of these two host-restricted species there was large-scale gene loss and inactivation; host adaptation seems to be a consequence of loss, not gain, of function, and differences in virulence may be related to loss of regulatory or control functions.