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Appl Environ Microbiol ; 75(15): 5111-20, 2009 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-19502440

RESUMEN

Soils harbor enormously diverse bacterial populations, and soil bacterial communities can vary greatly in composition across space. However, our understanding of the specific changes in soil bacterial community structure that occur across larger spatial scales is limited because most previous work has focused on either surveying a relatively small number of soils in detail or analyzing a larger number of soils with techniques that provide little detail about the phylogenetic structure of the bacterial communities. Here we used a bar-coded pyrosequencing technique to characterize bacterial communities in 88 soils from across North and South America, obtaining an average of 1,501 sequences per soil. We found that overall bacterial community composition, as measured by pairwise UniFrac distances, was significantly correlated with differences in soil pH (r = 0.79), largely driven by changes in the relative abundances of Acidobacteria, Actinobacteria, and Bacteroidetes across the range of soil pHs. In addition, soil pH explains a significant portion of the variability associated with observed changes in the phylogenetic structure within each dominant lineage. The overall phylogenetic diversity of the bacterial communities was also correlated with soil pH (R(2) = 0.50), with peak diversity in soils with near-neutral pHs. Together, these results suggest that the structure of soil bacterial communities is predictable, to some degree, across larger spatial scales, and the effect of soil pH on bacterial community composition is evident at even relatively coarse levels of taxonomic resolution.


Asunto(s)
Bacterias/clasificación , Bacterias/aislamiento & purificación , Biodiversidad , ADN Bacteriano/genética , Análisis de Secuencia de ADN/métodos , Microbiología del Suelo , Suelo/análisis , Bacterias/genética , ADN Bacteriano/química , Concentración de Iones de Hidrógeno , América del Norte , América del Sur
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