RESUMEN
Four isolates of an unclassified microaerophilic bacterium resembling Campylobacter species were characterized by growth requirements, microscopic examination, biochemical characteristics, antimicrobial susceptibility tests, and protein profile analysis. The unclassified isolates were differentiated from Campylobacter jejuni, Campylobacter coli, Campylobacter fetus subsp. fetus, Campylobacter laridis, Campylobacter pylori, and an ovine isolate. The bacterium was fusiform shaped with a corrugated surface due to the presence of periplasmic fibers and had multiple bipolar flagella. Biochemically, the bacterium was separated from the Campylobacter controls by its negative catalase reaction, negative nitrate reduction, and no growth in 1% glycine. It was also resistant to ampicillin. Protein profile analysis demonstrated nine major protein bands present in the unclassified isolates that were absent in the Campylobacter controls. The bacterium also differed from the ovine isolate by its negative catalase reaction, rapid urea hydrolysis, and susceptibility to clindamycin, erythromycin, and tetracycline. Our results showed that the unclassified bacterium was distinct from the recognized Campylobacter species.
Asunto(s)
Bacterias/clasificación , Enfermedades de los Perros/microbiología , Gastroenteritis/microbiología , Animales , Antibacterianos/farmacología , Bacterias/citología , Fenómenos Fisiológicos Bacterianos , Proteínas Bacterianas/análisis , Campylobacter/fisiología , Catalasa/metabolismo , Perros , Heces/microbiología , Gastroenteritis/veterinaria , Glicina/farmacología , Humanos , Nitratos/metabolismo , TemperaturaRESUMEN
An unusual microaerophilic gram-negative bacterium was isolated from the stools of two individuals presenting with chronic diarrhea. This bacterium resembled Campylobacter species by colonial morphology and biochemical reactions. However, microscopic examination revealed a fusiform rod with a corrugated surface, rather than a spiral rod. This is the first reported isolation of this bacterium from humans.