RESUMEN
Many crops are ill-protected against insect pests during storage. To protect cereal grains from herbivores during storage, pesticides are often applied. While pesticides have an undoubtable functionality, increasing concerns are arising about their application. In the present study, we investigated a bioinspired cyanogenic grain coating with amygdalin as cyanogenic precursor mimicking the feeding-triggered release of hydrogen cyanide (HCN) found for example in bitter almonds. The multilayer coating consisted of biodegradable polylactic acid with individual layers containing amygdalin or ß-glucosidase which is capable of degrading amygdalin to HCN. This reaction occurred only when the layers were ruptured, e.g., by a herbivore attack. Upon feeding coated cyanogenic wheat grains to Tenebrio molitor (mealworm beetle), Rhizopertha dominica (lesser grain borer), and Plodia interpunctella (Indianmeal moth), their reproduction as well as consumption rate were significantly reduced, whereas germination ability increased compared to noncoated grains. In field experiments, we observed an initial growth delay compared to uncoated grains which became negligible at later growth stages. The here shown strategy to artificially apply a naturally occurring defense mechanisms could be expanded to other crops than wheat and has the potential to replace certain pesticides with the benefit of complete biodegradability and increased safety during storage.
Asunto(s)
Escarabajos/efectos de los fármacos , Cianuro de Hidrógeno/farmacología , Mariposas Nocturnas/efectos de los fármacos , Extractos Vegetales/farmacología , Prunus/química , Triticum/parasitología , Amigdalina/farmacología , Animales , Escarabajos/fisiología , Conducta Alimentaria/efectos de los fármacos , Germinación/efectos de los fármacos , Mariposas Nocturnas/fisiología , Semillas/química , Semillas/crecimiento & desarrollo , Semillas/parasitología , Triticum/química , Triticum/crecimiento & desarrolloRESUMEN
Biomaterials made of biodegradable poly(α-hydroxyesters) such as poly(lactide-co-glycolide) (PLGA) are known to decrease the pH in the vicinity of the implants. Bioactive glass (BG) is being investigated as a counteracting agent buffering the acidic degradation products. However, in dentistry the question arises whether an antibacterial effect is rather obtained from pure PLGA or from BG/PLGA composites, as BG has been proved to be antimicrobial. In the present study the antimicrobial properties of electrospun PLGA and BG45S5/PLGA fibres were investigated using human oral bacteria (specified with mass spectrometry) incubated for up to 24 h. BG45S5 nanoparticles were prepared by flame spray synthesis. The change in colony-forming units (CFU) of the bacteria was correlated with the pH of the medium during incubation. The morphology and structure of the scaffolds as well as the appearance of the bacteria were followed bymicroscopy. Additionally, we studied if the presence of BG45S5 had an influence on the degradation speed of the polymer. Finally, it turned out that the pH increase induced by the presence of BG45S5 in the scaffold did not last long enough to show a reduction in CFU. On the contrary, pure PLGA demonstrated antibacterial properties that should be taken into consideration when designing biomaterials for dental applications.
Asunto(s)
Antibacterianos/farmacología , Bacterias/efectos de los fármacos , Cerámica/química , Vidrio/química , Implantes Experimentales , Ácido Láctico/química , Nanopartículas/química , Ácido Poliglicólico/química , Andamios del Tejido/química , Bacterias/crecimiento & desarrollo , Recuento de Colonia Microbiana , Humanos , Concentración de Iones de Hidrógeno/efectos de los fármacos , Pruebas de Sensibilidad Microbiana , Viabilidad Microbiana/efectos de los fármacos , Peso Molecular , Boca/microbiología , Nanopartículas/ultraestructura , Tamaño de la Partícula , Copolímero de Ácido Poliláctico-Ácido Poliglicólico , SolucionesRESUMEN
A mussel-inspired synthetic adhesive based on dopamine containing methacrylate copolymers was developed to bond polymers to metal surfaces at an adhesion strength of up to 20 MPa for bulk samples.