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1.
Allergol Immunopathol (Madr) ; 47(4): 372-377, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-31176517

RESUMEN

INTRODUCTION: Chronic granulomatous disease (CGD) is a disorder of phagocyte function, characterized by pyogenic infections and granuloma formation caused by defects in NADPH oxidase complex activity. Although the effect of CGD mainly reflects the phagocytic compartment, B cell responses are also impaired in patients with CGD. MATERIALS AND METHODS: Flow cytometric analysis was performed on peripheral blood samples from 35 CGD patients age-matched with healthy controls (HC). The target cells of our study were the naive (IgD+/CD27-), memory (IgD-/CD27+), and B1a (CD5+) cells. Immunoglobulins (Igs) were also measured. This study was performed in a Latin American cohort. RESULTS: We found significantly higher levels of naive B cells and B1a cells, but lower levels of memory B cells were found in CGD patients compared to HC. There was no significant difference of cell percentages per inheritance type. DISCUSSION: Our findings suggest that the deficiency of NADPH oxidase components can affect the differentiation of naive B cells to memory B cells. Consequently, memory cells will be low, which also influenced the expression of CD27 in memory B cells and as a result, the percentage of naive cells increases. An altered phenotype of B lymphocytes in CGD patients may contribute to the opportunistic infections and autoimmune disorders that are seen in this disease.


Asunto(s)
Subgrupos de Linfocitos B/inmunología , Linfocitos B/inmunología , Enfermedad Granulomatosa Crónica/inmunología , NADPH Oxidasa 2/genética , Adolescente , Adulto , Separación Celular , Niño , Preescolar , Estudios de Cohortes , Femenino , Citometría de Flujo , Enfermedad Granulomatosa Crónica/genética , Humanos , Memoria Inmunológica , Inmunofenotipificación , Lactante , Masculino , México , Miembro 7 de la Superfamilia de Receptores de Factores de Necrosis Tumoral/metabolismo , Adulto Joven
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