RESUMEN
Blasts phenotype was determined in 61 children with the acute lymphoblastic leukemia. Non-T-cell acute lymphoblastic leukemia was diagnosed in 51 children. Stages of blasts differentiation were determined with the aid of monoclonal antibodies set using alkaline phosphatase-anti-alkaline phosphatase technique. Blasts in 50 patients belonged to B subpopulation confirmed by the presence of panB CD19 and CD22 antigens. Common antigen was seen in 76.5% of the examined patients with non-T-cell acute lymphoblastic leukemia. Cases of non-T-cell acute lymphoblastic leukemia were divided into 8 subgroups depending on the antigens of B-cells differentiation. An identification of pre-B subgroups of the acute lymphoblastic leukemia indicates heterogenicity of the acute lymphoblastic leukemias in childhood and enables their classification into groups corresponding to the early stages of lymphoblasts maturation.
Asunto(s)
Antígenos de Diferenciación de Linfocitos B/inmunología , Linfocitos B/inmunología , Linfoma de Burkitt/inmunología , Anticuerpos Monoclonales/inmunología , Antígenos de Diferenciación de Linfocitos B/genética , Linfocitos B/patología , Biomarcadores de Tumor/genética , Biomarcadores de Tumor/inmunología , Linfoma de Burkitt/diagnóstico , Linfoma de Burkitt/genética , Diferenciación Celular/fisiología , Niño , Femenino , Humanos , Masculino , FenotipoRESUMEN
Serum IgA, IgM and IgG as well as percentage of lymphocytes T and B in peripheral blood were determined in 50 children aged between 6 months and 3 years with iron deficiency anaemia. A significant decrease in lymphocyte T number in these children was found in comparison with control group of healthy children. Lymphocyte T count positively correlated with serum iron concentration. Moreover, a decrease in serum IgA and IgG was found in children with iron deficit.
Asunto(s)
Anemia Hipocrómica/inmunología , Linfocitos B/inmunología , Inmunoglobulinas/análisis , Linfocitos T/inmunología , Preescolar , Disgammaglobulinemia/etiología , Humanos , Deficiencia de IgA , Deficiencia de IgG , Lactante , Recuento de Leucocitos , Linfopenia/etiologíaRESUMEN
The studied group comprised 38 boys and girls aged from 7 months to 5 years with chronic pyelonephritis. Counts of B and T-cells and serum concentrations of IgG, IgM, IgA were determined. A significant depression was noted of the per cent proportion of T-cells in relation to the control group of healthy children with a significantly lower IgM concentration without significant changes in IgG and IgA levels.
Asunto(s)
Pielonefritis/inmunología , Formación de Anticuerpos , Preescolar , Enfermedad Crónica , Femenino , Humanos , Inmunidad Celular , Inmunoglobulina G/análisis , Lactante , Recuento de Leucocitos , Masculino , Linfocitos TRESUMEN
From January 1987 to April 1988 six children we studied cytologically with the use of alkaline phosphatase-anti-alkaline phosphatase (APAAP) method and monoclonal antibodies (MoP) in order to establish the diagnosis of Non-Hodgkin Lymphoma. (NHL). Cytologic studies concerned pleural fluid (3 patients) imprints of the lymph node (3 patients), bone marrow smears (2 patients) and cerebrospinal fluid (1 patient). We performed simultaneously routine cytologic and histopathologic studies of the lymph nodes. Antigen T6 (thymocytes) was present in blasts of all patients, which permitted us to classify the blasts as common stage II group according to Reinherz. In all cases we found at least two positive antigen detected by MoP pan T (CD2, CD3, CD5, CD7) in one case--no expression of antigen T3 (CD3) and in two cases no antigen detected by an antibody CD2. Antigen Ia was found in one patient, and weak expression of antigen CALLA (CD10) in one patients. In three patients we showed a simultaneous expression of antigens T4, T8, whereas in two patients they were not observed. One child possessed mature phenotype T4, T6. By using APAAP method with MoP in cytologic studies it was possible to diagnose T-lymphoblastic lymphoma in six children before the results of histopathologic examination of the lymph nodes.
Asunto(s)
Fosfatasa Alcalina , Leucemia-Linfoma de Células T del Adulto/diagnóstico , Linfoma no Hodgkin/diagnóstico , Anticuerpos Monoclonales , Médula Ósea/patología , Líquido Cefalorraquídeo/citología , Niño , Preescolar , Diagnóstico Diferencial , Femenino , Humanos , Técnicas para Inmunoenzimas , Leucemia-Linfoma de Células T del Adulto/líquido cefalorraquídeo , Leucemia-Linfoma de Células T del Adulto/patología , Ganglios Linfáticos/patología , Linfoma no Hodgkin/líquido cefalorraquídeo , Linfoma no Hodgkin/patología , Masculino , Derrame Pleural/patologíaAsunto(s)
Infecciones Bacterianas/sangre , Bronquitis/sangre , Neumonía/sangre , Transferrina/análisis , Enfermedad Aguda , Preescolar , Humanos , LactanteAsunto(s)
Quimiotaxis de Leucocito , Leucemia Linfoide/sangre , Niño , Preescolar , Granulocitos/fisiología , Humanos , LactanteAsunto(s)
Anemia Hipocrómica/epidemiología , Hierro/sangre , Factores de Edad , Humanos , Lactante , PoloniaRESUMEN
A method was elaborated to assess the transplacental passage of inhalatory anaesthetic agents--diethyl ether and halothane during Caesarean section. The method is based on measurements of blood concentrations of these agents in material venous blood and in arterial and venous blood taken from the umbilical cord during the operation. Halothane and diethyl ether concentrations were determined by means of gas chromatography. The studies were carried out on 2 groups of 25 cases each. The obtained results indicate that diethyl ether passes in a higher degree across the placenta than halothane. The mean ratio of halothane concentrations in foetal venous blood to maternal venous blood determined in 25 cases was 0.664 and diethyl ether ratio was 0.988.