RESUMEN
1. The effects of continuous gamma radiation on the viability of Trichomonas vaginalis (ATCC 30001) were assessed by a colony count technique. 2. A triphasic survival curve showed an initial shoulder (Dq) of 3 Gy followed by three linear curves with D0 values of 34, 300, and 90 Gy. 3. Sterilization of 10(6) cells/ml occurred from 1600 to 1800 Gy of radiation. 4. Population growth, subsequent to radiation exposure of 17-100 Gy, showed an increased lag time followed by a faster rate of growth, compared with unirradiated cells. 5. Trichomonas vaginalis is more sensitive to ionizing radiation than free-living protozoa and appears as radiosensitive as those parasitic protozoa examined in radioattenuation experiments.
Asunto(s)
Trichomonas vaginalis/efectos de la radiación , Animales , Rayos gamma , Trichomonas vaginalis/crecimiento & desarrolloRESUMEN
Histone and non-histone chromosomal proteins from human diploid fibroblasts of different in vitro ages were extracted and subjected to SDS--polycrylamide gel electrophoresis. The proteins were taken from cells maintained in three distinct culture states: preconfluent (log phase of growth), confluent (stationary phase of growth), and arrested (presumptive G0 phase). Age associated alterations in the incorporation of radioactive amino acids were detected in the fractionated proteins in every culture state but were less pronounced during the arrested state. Age related difference detected under growth conditions may reflect variations in the proliferative nature of the populations. Differences seen during the arrested state may be indicative of basic changes in the chromosomal protein complement of different age populations.
Asunto(s)
Proteínas Cromosómicas no Histona/metabolismo , Histonas/metabolismo , Aminoácidos/metabolismo , Células Cultivadas , Proteínas Cromosómicas no Histona/análisis , Fibroblastos/crecimiento & desarrollo , Fibroblastos/metabolismo , Histonas/análisis , Humanos , Factores de TiempoRESUMEN
Human fibroblasts derived from newborn foreskin and designated CF-3 were assayed for heat labile glucose-6-phosphate dehydrogenase (G6PD) when they had grown to confluency, as well as when they were arrested in an essentially nonmitotic state. Under both culture conditions there was an increase in heat labile G6PD (up to 25% of the total activity) as cells progressed through their in vitro lifespan; however, arrested cells exhibited less heat labile G6PD than did comparable growth controls (5-10% decrease). Acrylamide gel electrophoresis of crude G6PD preparations revealed three distinct bands of enzymatic activity. One of the bands was tentatively identified as the dimeric form of the enzyme and another as the tetrameric form. The tetrameric form was more heat sensitive, and the percent of the total activity of this form increased as the cells became senescent. The percent of total activity of the tetrameric form was comparable to the percent heat labile enzyme assayed at a given population doubling level. These results indicated that the observed increase in heat lability of G6PD with age may be due to a shift in equilibrium to the tetrameric form of the enzyme rather than the synthesis of aberrant enzyme molecules.
Asunto(s)
Supervivencia Celular , Glucosafosfato Deshidrogenasa/metabolismo , División Celular , Línea Celular , Electroforesis en Gel de Poliacrilamida , Glucosafosfato Deshidrogenasa/análisis , Calor , Conformación MolecularRESUMEN
Protein synthesis and turnover were measured in human diploid fibroblasts which were arrested in an essentially nonmitotic state by reducing the serum concentration in the incubation medium to 0.5%. Through the first 4 days of the arrested period both early and late passage cells lost about 20% of their cellular protein. There was a reduction in the rate of protein synthesis at both passage levels during this period, but there was no significant age-related difference in the synthetic rate or the rate of protein turnover. After day 4 both early and late passage cells maintained a constant protein content, but late passage cells did this while processing more protein through faster rates of both synthesis and turnover than did early passage cells. These results support those theories of cellular senescence which predict altered protein metabolism as a major consequence of the aging process.