RESUMEN
BACKGROUND: Polycystic ovary syndrome (PCOS) is an endocrine disorder in women and this chronic low-grade inflammatory state is often associated with various metabolic disorders such as type 2 diabetes mellitus, cardiovascular disorders, and other complications. METHODS: The current study was aimed to assess inflammatory markers including high-sensitivity C-reactive protein (hs-CRP) and complement element 3 (C3) in PCOS subjects and to investigate the association of these inflammatory markers with insulin resistance. One hundred and fifty PCOS subjects and 150 healthy women as control subjects were recruited for the investigation. Serum was used for investigating the routine and specific parameters. RESULTS: The mean serum hs-CRP was significantly higher (p < 0.0001) in PCOS subjects (10,738 ± 7,658 ng/mL) as compared to the healthy control subjects (1,128 ± 695.92 ng/mL) and mean serum C3 was also highly significant (p < 0.0001) in PCOS subjects (206.86 ± 21.64 mg/dL) as compared to the healthy control subjects (91.84 ± 16.02 mg/dL). Fasting blood sugar, serum insulin, insulin resistance, and BMI were significantly higher (p < 0.0001) as compared to the control subjects. Serum hs-CRP was positively correlated with fasting blood sugar (r = 0.630, p < 0.0001), serum insulin (r = 0.700, p < 0.0001) and insulin resistance (r = 0.694, p < 0.0001) in PCOS. The serum C3 was also positively correlated with fasting blood sugar (r = 0.352, p < 0.0001), serum insulin (r = 0.379, p < 0.0001) and insulin resistance (r = 0.378, p < 0.0001) in PCOS. CONCLUSIONS: A strong association of serum C3 and hs-CRP with serum insulin and insulin resistance in PCOS subjects indicates that these inflammatory markers may serve as the most significant predictor of the risk of future diabetes mellitus and cardiovascular complications in PCOS.
Asunto(s)
Diabetes Mellitus Tipo 2 , Resistencia a la Insulina , Síndrome del Ovario Poliquístico , Índice de Masa Corporal , Proteína C-Reactiva/análisis , Femenino , Humanos , Insulina , Síndrome del Ovario Poliquístico/diagnósticoRESUMEN
BACKGROUND: A number of experimental studies have been put forth suggesting an important role of the hemostatic system in acute pancreatitis (AP) in the recent past. However, meaningful studies on clinical values of parameters of the hemostatic system in predicting pancreatitis associated complications are still scarce. In the current investigation, we evaluated the role of D-dimer to predict the severity of acute pancreatitis on day 1 of admission to the hospital. METHODS: A total of 160 subjects (75 mild AP + 35 severe AP + 50 healthy controls) were examined in the study. Biochemical and hemostatic parameters were compared between various groups of subjects on day 1 and day 3 of admission to the hospital. RESULTS: Levels of prothrombin time (PT), fibrinogen, D-dimer, and C-reactive protein (CRP) were significantly higher in the severe AP group than in the mild AP group. Antithrombin III (AT III) levels were significantly lower in the severe AP group than in the mild AP group. D-dimer levels were 5 times higher than the reference limit in the severe group and 1.7 times higher than the reference limit in the mild group. This difference was statistically highly significant (< 0.0001). A positive correlation between D-dimer and CRP, D-dimer and fibrinogen, and between D-dimer and PT was recorded. CONCLUSIONS: Estimation of the levels of D-dimer on admission day provides an accurate method for the identification of patients who will develop systemic complications in the further course of AP.
Asunto(s)
Productos de Degradación de Fibrina-Fibrinógeno/análisis , Pancreatitis/sangre , Enfermedad Aguda , Adulto , Anciano , Biomarcadores/sangre , Pruebas de Coagulación Sanguínea , Proteína C-Reactiva/análisis , Femenino , Fibrinógeno/análisis , Humanos , Masculino , Persona de Mediana Edad , Pancreatitis Alcohólica/sangre , Pronóstico , Índice de Severidad de la EnfermedadRESUMEN
BACKGROUND: Increasing evidence in both experimental and clinical studies suggests that free radical mediated oxidative stress plays a major role in the pathogenesis of both types of diabetes mellitus. Proteins and lipids are among the prime targets for oxidative stress. In this study we evaluated oxidative stress in Type 1 Diabetes Mellitus (insulin dependent diabetes mellitus, IDDM) patients by estimating lipid peroxidation and the effect of vitamin E on oxidative stress and metabolic parameters. METHODS: A total of 40 children (20 Type 1 Diabetes Mellitus patients + 20 healthy controls) were examined in the study. Oxidative stress parameters malondialdehyde (MDA), antioxidants, reduced glutathione (GSH), vitamin E and metabolic parameters were studied. All the type 1 Diabetes Mellitus patients were supplemented with 600 mg/daily vitamin E for three months. After three months of supplementation all the parameters mentioned above were studied again. RESULTS: Reduced glutathione and vitamin E levels were lower and malondialdehyde levels were higher in Type 1 Diabetes Mellitus patients compared to healthy controls (p < 0.05). After supplementation with vitamin E in diabetic patients a significant decrease (p < 0.05) in MDA levels and significant increase in GSH (p < 0.05) and vitamin E (p < 0.05) levels were found. A negative correlation between MDA and vitamin E, between MDA and GSH and a positive correlation between vitamin E and GSH was found. Significant changes were not observed in metabolic parameters in Type 1 Diabetes Mellitus patients after vitamin E supplementation (p > 0.05). CONCLUSIONS: Vitamin E ameliorates oxidative stress in Type 1 Diabetes Mellitus patients and improves antioxidant defense system. However, vitamin E does not have any advantage for metabolic parameters.