RESUMEN
Microinjection of either type 1 human adenovirus, type SA7 monkey adenovirus virions or circular adenovirus DNA, obtained by the treatment of DNA-terminal protein complexes with glutaraldehyde, into nuclei of permissive cells results in the complete cycle of virus reproduction. Microinjection of neither linear native, condensed adenovirus DNA nor the DNA-terminal protein complexes under the same conditions initiates the adenovirus reproduction thought the synthesis of early and some late viral antigens is observed in the injected cells. Integration of injected adenovirus DNA into the cellular DNA occurs as far as 30 min after injection. Microinjection of either adenovirus DNA or its oncogene containing fragments into nuclei of semipermissive cells induces the transformation of these cells. In this case the time of the first appearance of transformation foci is decreased.
Asunto(s)
Adenoviridae/genética , ADN Recombinante/genética , ADN Viral/genética , Transfección , Virión/genética , Animales , Autorradiografía , Transformación Celular Neoplásica/genética , Transformación Celular Viral/genética , Células Cultivadas , ADN Viral/ultraestructura , Electroforesis en Gel de Agar , Técnica del Anticuerpo Fluorescente , Humanos , Microinyecciones , Hibridación de Ácido Nucleico , Plásmidos , Timidina Quinasa/genética , Proteínas Virales/metabolismoRESUMEN
Physico-chemical characteristics of PBV-5 virus and its nucleic acid were studied. Morphologically, the virus is a rectilinear polyhedron 270 A in diameter, without a process. The sedimentation constant of a virus particle is 117S, the buoyant density of the virus in cesium chloride density is 1.437 g/cm3. As shown by qualitative reactions with orcinol and diphenylamine, the virus contains DNA. The viral DNA preparation in CsCl gradient solution showed the buoyant density 1.719 g/cm3. Electron microscope examination of a PBV-5 DNA preparation showed the nucleic acid molecules to be single-stranded rings.