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1.
Sensors (Basel) ; 24(14)2024 Jul 19.
Artículo en Inglés | MEDLINE | ID: mdl-39066082

RESUMEN

Providing safe, smooth, and efficient trajectories for autonomous vehicles has long been a question of great interest in the field of autopiloting. In dynamic and ever-changing urban environments, safe and efficient trajectory planning is fundamental to achieving autonomous driving. Nevertheless, the complexity of environments with multiple constraints poses challenges for trajectory planning. It is possible that behavior planners may not successfully obtain collision-free trajectories in complex urban environments. Herein, this paper introduces spatio-temporal joint optimization-based trajectory planning (SJOTP) with multi-constraints for complex urban environments. The behavior planner generates initial trajectory clusters based on the current state of the vehicle, and a topology-guided hybrid A* algorithm applied to an inflated map is utilized to address the risk of collisions between the initial trajectories and static obstacles. Taking into consideration obstacles, road surface adhesion coefficients, and vehicle dynamics constraints, multi-constraint multi-objective coordinated trajectory planning is conducted, using both differential-flatness vehicle models and point-mass vehicle models. Taking into consideration longitudinal and lateral coupling in trajectory optimization, a spatio-temporal joint optimization solver is used to obtain the optimal trajectory. The simulation verification was conducted on a multi-agent simulation platform. The results demonstrate that this methodology can obtain optimal trajectories safely and efficiently in complex urban environments.

2.
Biosci Rep ; 44(3)2024 Mar 29.
Artículo en Inglés | MEDLINE | ID: mdl-38415405

RESUMEN

Endometrial carcinoma (EC) is a common malignancy that originates from the endometrium and grows in the female reproductive system. Surgeries, as current treatments for cancer, however, cannot meet the fertility needs of young women patients. Thus, progesterone (P4) therapy is indispensable due to its effective temporary preservation of female fertility. Many cancer cells are often accompanied by changes in metabolic phenotypes, and abnormally dependent on the amino acid glutamine. However, whether P4 exerts an effect on EC via glutamine metabolism is unknown. In the present study, we found that P4 could inhibit glutamine metabolism in EC cells and down-regulate the expression of the glutamine transporter ASCT2. This regulation of ASCT2 affects the uptake of glutamine. Furthermore, the in vivo xenograft studies showed that P4 inhibited tumor growth and the expression of key enzymes involved in glutamine metabolism. Our study demonstrated that the direct regulation of glutamine metabolism by P4 and its anticancer effect was mediated through the inhibition of ASCT2. These results provide a mechanism underlying the effects of P4 therapy on EC from the perspective of glutamine metabolism.


Asunto(s)
Sistema de Transporte de Aminoácidos ASC , Neoplasias Endometriales , Glutamina , Progesterona , Femenino , Humanos , Línea Celular Tumoral , Proliferación Celular/genética , Neoplasias Endometriales/tratamiento farmacológico , Glutamina/antagonistas & inhibidores , Glutamina/metabolismo , Progesterona/farmacología , Progesterona/uso terapéutico , Sistema de Transporte de Aminoácidos ASC/efectos de los fármacos , Sistema de Transporte de Aminoácidos ASC/metabolismo , Antígenos de Histocompatibilidad Menor
3.
J Adv Res ; 37: 119-131, 2022 03.
Artículo en Inglés | MEDLINE | ID: mdl-35499042

RESUMEN

Introduction: O-linked ß-D-N-acetylglucosamine (O-GlcNAc) modification is a post-translational modification in which a single O-GlcNAc is added to serine or threonine residues in nuclear, cytoplasmic, and mitochondrial proteins, and is involved in a variety of physiological processes. Objectives: In the present study, the role of O-GlcNAcylation in embryo implantation was evaluated. Furthermore, whether O-GlcNAcylation is involved in orchestrating glucose metabolism to influence endometrial cell physiological functions was investigated. Methods: Different endometrial tissues were detected using immunohistochemistry. Pregnant mouse models were established to verify molecular expression. O-GlcNAc transferase and aquaporin 3 (AQP3) knockdown were used to detect embryo implantation efficiency in vitro and in vivo. Western blotting and immunofluorescence were used to detect protein expression and stability. Dual luciferase reporter assay and chromatin immunoprecipitation (ChIP) were used to verify the binding transcription factor. Glycolysis was detected using bioenergy analyzer, and metabolites were analyzed using isotope 13C-labeled LC-MS. Metabolic-related genes were determined using RNA sequencing. Results: Activation of endometrial hexosamine biosynthetic pathway (HBP) caused elevated O-GlcNAcylation during the window of implantation, affecting endometrial cell function and embryo implantation. Specifically, elevated O-GlcNAcylation increased glucose uptake via glucose transporter 1 (GLUT1) leading to glucose metabolic flow into the pentose phosphate pathways and HBP, which regulate the metabolic reprogramming of endometrial cells. Furthermore, O-GlcNAcylation mediated the intracellular transport of glycerol to support and compensate for glycolysis through regulation of AQP3. Unexpectedly, elevated AQP3 also increased glucose uptake via GLUT1. These processes maintained higher metabolic requirements for endometrial physiology. Furthermore, the transcription factor SP1 specifically bound to the AQP3 promoter region, and O-GlcNAcylation of SP1 increased its stability and transcriptional regulation of AQP3 which is associated with O-GlcNAcylation of SP1. Conclusion: Overall, O-GlcNAcylation regulated glucose metabolism in endometrial cells, and AQP3-mediated compensation provides new insights into the communication between glycolysis and O-GlcNAcylation.


Asunto(s)
Acuaporina 3 , Glucólisis , Animales , Acuaporina 3/metabolismo , Implantación del Embrión , Glucosa/metabolismo , Transportador de Glucosa de Tipo 1/metabolismo , Hexosaminas , Ratones
4.
J Am Chem Soc ; 144(8): 3535-3542, 2022 Mar 02.
Artículo en Inglés | MEDLINE | ID: mdl-35107999

RESUMEN

Identification of catalytic active sites is pivotal in the design of highly effective heterogeneous metal catalysts, especially for structure-sensitive reactions. Downsizing the dimension of the metal species on the catalyst increases the dispersion, which is maximized when the metal exists as single atoms, namely, single-atom catalysts (SACs). SACs have been reported to be efficient for various catalytic reactions. We show here that the Pt SACs, although with the highest metal atom utilization efficiency, are totally inactive in the cyclohexane (C6H12) dehydrogenation reaction, an important reaction that could enable efficient hydrogen transportation. Instead, catalysts enriched with fully exposed few-atom Pt ensembles, with a Pt-Pt coordination number of around 2, achieve the optimal catalytic performance. The superior performance of a fully exposed few-atom ensemble catalyst is attributed to its high d-band center, multiple neighboring metal sites, and weak binding of the product.

5.
Chemistry ; 28(19): e202104623, 2022 Apr 01.
Artículo en Inglés | MEDLINE | ID: mdl-35157331

RESUMEN

Photo-thermal catalysis has been an attractive alternative strategy to promote chemical reactions for years, however, how light cooperates with thermal energy is still unclear. We meet this demand by exploring reaction mechanism via pressure dependency studies as well as H/D exchange experiments with HCOOH decomposition as a probe over a palladium nanoparticle (Pdn ) and isolated Pd (Pd1 ) decorated LaCrO3 /C3 N4 composite catalyst, in which the H2 formation rate shows a first-order dependence on HCOOH and inverse first-order dependence on CO partial pressures no matter the reaction was driven by thermal or photo-thermal energy. Additionally, negligible kinetic isotopic effects (KIEs: kH /kD ) were determined under both dark and light conditions at 1.04 and 1.18 when the HCOOH was replaced by HCOOD. Besides, when the reactant HCOOH was further replaced by DCOOD, the KIE values of 1.55 (dark) and 1.92 (light) were obtained, which indicates that the HCOOH decomposition follows kinetically relevant (KR) of C-H bond rupture within HCOOH molecule under both thermal and photo-thermal reaction conditions and the catalytic surface was found to be densely covered by CO based on the pressure dependency studies as well as the in situ Fourier transform infrared spectroscopy (FTIR) analysis. Clearly, the HCOOH decomposition driven by thermal and photo-thermal energy follows the same reaction mechanism. Nevertheless, light induced hot electrons and the derived thermal effect do cause the enhancement of the reaction activity in some circumstances compared with bare thermal catalysis, which clarifies the confusion on cooperation mechanism of photo and thermal energies from the kinetic perspective. Hot electrons induced by photo-illumination was confirmed by in situ FTIR CO chemisorption with ∼10 cm-1 redshift identified of the CO feature once light was introduced. Meanwhile, the photo thermal reaction system suffers from severe electron-hole re-combination at high reaction temperatures and make the thermal effect of photo irradiation dominant with respect to the effect at low reaction temperatures. This research provides insight to the mechanism on how photo-thermal reaction works and draws attention to the photo-thermal reaction process in boosting catalytic activity.

6.
Cell Biol Int ; 45(9): 1957-1965, 2021 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-34003541

RESUMEN

The successful implantation of embryos is crucial for pregnancy in mammals. This complex process is inevitably dependent on the development of the endometrium. The paired-like homeodomain transcription factor 2 (PITX2) is involved in a variety of biological processes, but whether it is involved in embryo implantation has not been reported. In this study, we aimed to investigate uterine expression and regulation of PITX2 during implantation. We found that PITX2 was elevated in the human endometrium in the secretory phase. The results of the pregnant mouse models showed that PITX2 expression was spatiotemporal in mouse endometrial tissue throughout peri-implantation period, and it was significantly upregulated at the time of implantation. Interestingly, PITX2 was mainly localized to the glandular epithelium cells on D2.5-3.5 of pregnancy, while D5.5-6.5 was largely expressed in stromal cells. In vitro, PITX2 regulated endometrial cells proliferation, migration, invasion, and other functions through the Wnt/ß-catenin signaling pathway. In addition, a significant decrease in the rate of embryo implantation was observed after injecting PITX2 small interfering RNA into the uterine horn. These results demonstrate the effects of PITX2 on the physiological function of endometrial cells and embryo implantation, suggesting a role in the endometrial regulatory mechanism during implantation.


Asunto(s)
Implantación del Embrión , Endometrio/metabolismo , Proteínas de Homeodominio/fisiología , Factores de Transcripción/fisiología , Vía de Señalización Wnt , Adulto , Animales , Línea Celular , Femenino , Humanos , Ratones , Ratones Endogámicos C57BL , Persona de Mediana Edad , Embarazo , Proteína del Homeodomínio PITX2
7.
Int J Mol Sci ; 17(2)2016 Jan 26.
Artículo en Inglés | MEDLINE | ID: mdl-26821021

RESUMEN

We previously reported that arsenic (As) impaired learning and memory by down-regulating calmodulin-dependent protein kinase IV (CaMK IV) in mouse cerebellum. It has been documented that the thyroid hormone receptor (TR)/retinoid X receptor (RXR) heterodimer and thyroid hormone (TH) may be involved in the regulation of CaMK IV. To investigate whether As affects the TR/RXR heterodimer and TH, we determined As concentration in serum and cerebellum, 3,5,3'-triiodothyronine (T3) and thyroxin (T4) levels in serum, and expression of CaMK IV, TR and RXR in cerebellum of mice exposed to As. Cognition function was examined by the step-down passive avoidance task and Morris water maze (MWM) tests. Morphology of the cerebellum was observed by Hematoxylin-Eosin staining under light microscope. Our results showed that the concentrations of As in the serum and cerebellum of mice both increased with increasing As-exposure level. A significant positive correlation was found between the two processes. Adeficit in learning and memory was found in the exposed mice. Abnormal morphologic changes of Purkinje cells were observed in cerebellum of the exposed mice. Moreover, the cerebellar expressions of CaMK IV protein and the TRß gene, and TRß1 protein were significantly lower in As-exposed mice than those in controls. Subchronic exposure to As appears to increase its level in serum and cerebella of mice, impairing learning and memory and down-regulating expression of TRß1 as well as down-stream CaMK IV. It is also suggested that the increased As may be responsible for down-regulation of TRß1 and CaMK IV in cerebellum and that the down-regulated TRß1 may be involved in As-induced impairment of learning and memory via inhibiting CaMK IV and its down-stream pathway.


Asunto(s)
Arsénico/toxicidad , Cerebelo/efectos de los fármacos , Discapacidades para el Aprendizaje/inducido químicamente , Trastornos de la Memoria/inducido químicamente , Transducción de Señal/efectos de los fármacos , Animales , Proteína Quinasa Tipo 4 Dependiente de Calcio Calmodulina/metabolismo , Cerebelo/metabolismo , Cerebelo/patología , Regulación de la Expresión Génica/efectos de los fármacos , Discapacidades para el Aprendizaje/patología , Discapacidades para el Aprendizaje/fisiopatología , Aprendizaje por Laberinto/efectos de los fármacos , Trastornos de la Memoria/patología , Trastornos de la Memoria/fisiopatología , Ratones , Receptores X Retinoide/metabolismo , Receptores beta de Hormona Tiroidea/metabolismo , Hormonas Tiroideas/metabolismo , Pruebas de Toxicidad Subcrónica
8.
J Med Syst ; 29(4): 413-23, 2005 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-16178338

RESUMEN

With the evolving and diverse electronic medical record (EMR) systems, there appears to be an ever greater need to link EMR systems and patient accounting systems with a standardized data exchange format. To this end, the CLinical Accounting InforMation (CLAIM) data exchange standard was developed. CLAIM is subordinate to the Medical Markup Language (MML) standard, which allows the exchange of medical data among different medical institutions. CLAIM uses eXtensible Markup Language (XML) as a meta-language. The current version, 2.1, inherited the basic structure of MML 2.x and contains two modules including information related to registration, appointment, procedure and charging. CLAIM 2.1 was implemented successfully in Japan in 2001. Consequently, it was confirmed that CLAIM could be used as an effective data exchange format between EMR systems and patient accounting systems.


Asunto(s)
Contabilidad de Pagos y Cobros , Sistemas de Registros Médicos Computarizados , Programas Informáticos , Integración de Sistemas , Formulario de Reclamación de Seguro , Japón , Lenguajes de Programación
9.
J Med Syst ; 29(5): 463-71, 2005 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-16180482

RESUMEN

CLinical Accounting InforMation (CLAIM) is a standard for the exchange of data between patient accounting systems and electronic medical record (EMR) systems. It uses eXtensible Markup Language (XML) as a meta-language and was developed in Japan. CLAIM is subordinate to the Medical Markup Language (MML) standard, which allows the exchange of medical data between different medical institutions. It has inherited the basic structure of MML 2.x and the current version, version 2.1, contains two modules and nine data definition tables. In China, no data exchange standard yet exists that links EMR systems to accounting systems. Taking advantage of CLAIM's flexibility, we created a localized Chinese version based on CLAIM 2.1. Since Chinese receipt systems differ from those of Japan, some information such as prescription formats, etc. are also different from those in Japan. Two CLAIM modules were re-engineered and six data definition tables were either added or redefined. The Chinese version of CLAIM takes local needs into account, and consequently it is now possible to transfer data between the patient accounting systems and EMR systems of Chinese medical institutions effectively.


Asunto(s)
Contabilidad , Sistemas de Información/instrumentación , Lenguaje , Diseño de Software , Contabilidad/métodos , Contabilidad/organización & administración , China , Humanos , Sistemas de Información/organización & administración , Sistemas de Registros Médicos Computarizados/instrumentación , Sistemas de Registros Médicos Computarizados/organización & administración
10.
J Med Syst ; 29(5): 555-67, 2005 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-16180490

RESUMEN

Medical Markup Language (MML) is a standard for the exchange of medical data among different medical institutions. It was developed in Japan in 1995. Since version 2.21, MML has used eXtensible Markup Language (XML) as a meta-language. The latest version, 3.0, conforms to HL7 Clinical Document Architecture (CDA) and contains 14 modules and 36 data definition tables. In China, a standard which structures entire medical records in XML does not yet exist. Taking advantage of MML's flexibility, we created a localized Chinese version based on MML 3.0. Parts of the original specifications have been enhanced; these include a newly developed health insurance information module and 12 additional or redefined data definition tables. The Chinese version takes local needs into account and now makes it possible to exchange medical data among Chinese medical institutions.


Asunto(s)
Lenguaje , Registro Médico Coordinado/instrumentación , Diseño de Software , China , Humanos , Registro Médico Coordinado/normas , Programas Nacionales de Salud
11.
J Med Syst ; 28(6): 523-33, 2004 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-15615281

RESUMEN

Medical Markup Language (MML), as a set of standards, has been developed over the last 8 years to allow the exchange of medical data between different medical information providers. MML Version 2.21 used XML as a metalanguage and was announced in 1999. In 2001, MML was updated to Version 2.3, which contained 12 modules. The latest version--Version 3.0--is based on the HL7 Clinical Document Architecture (CDA). During the development of this new version, the structure of MML Version 2.3 was analyzed, subdivided into several categories, and redefined so the information defined in MML could be described in HL7 CDA Level One. As a result of this development, it has become possible to exchange MML Version 3.0 medical documents via HL7 messages.


Asunto(s)
Aplicaciones de la Informática Médica , Computación en Informática Médica , Sistemas de Registros Médicos Computarizados/normas , Lenguajes de Programación , Registro Médico Coordinado/normas , Diseño de Software
12.
J Med Syst ; 28(6): 689-710, 2004 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-15615296

RESUMEN

Since a little before 2000, hospital cost accounting has been increasingly performed at Japanese national university hospitals. At Kumamoto University Hospital, for instance, departmental costs have been analyzed since 2000. And, since 2003, the cost balance has been obtained according to certain diseases for the preparation of Diagnosis-Related Groups and Prospective Payment System. On the basis of these experiences, we have constructed a simulation model of hospital management. This program has worked correctly at repeated trials and with satisfactory speed. Although there has been room for improvement of detailed accounts and cost accounting engine, the basic model has proved satisfactory. We have constructed a hospital management model based on the financial data of an existing hospital. We will later improve this program from the viewpoint of construction and using more various data of hospital management. A prospective outlook may be obtained for the practical application of this hospital management model.


Asunto(s)
Contabilidad/métodos , Asignación de Costos/métodos , Grupos Diagnósticos Relacionados/economía , Administración Financiera de Hospitales/métodos , Costos de Hospital/clasificación , Hospitales Universitarios/economía , Modelos Econométricos , Simulación por Computador , Eficiencia Organizacional , Departamentos de Hospitales/economía , Humanos , Japón , Innovación Organizacional , Diseño de Software
13.
J Med Syst ; 28(5): 437-45, 2004 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-15527031

RESUMEN

Cost accounting according to diagnoses covering approximately 600 inpatients with 64 diseases in 20 departments of Kumamoto University was carried out. The reports of these results were automatically generated and used for individual departmental meetings with participating delegates. The administration of each department as well as the management of diseases was discussed at the meetings, and all departments were requested to provide a report of their discussions. We are planning to increase the number of patients in the sample group and to perform more comprehensive and accurate hospital cost accounting.


Asunto(s)
Costos y Análisis de Costo/métodos , Enfermedad/clasificación , Hospitales Universitarios/economía , Grupos Diagnósticos Relacionados , Humanos , Japón
14.
J Med Syst ; 27(4): 357-66, 2003 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-12846467

RESUMEN

As a set of standards, Medical Markup Language (MML) has been developed over the last 8 years to allow the exchange of medical data between different medical information providers MML version 2.21 was characterized by XML as metalanguage and was announced in 1999, at which time full-scale implementation tests were carried out; subsequently, various information and functional inadequacies were discovered in this version. MML was therefore updated to version 2.3 in 2001. At present, MML contains 12 MML modules including the new referral, test result, and report modules. In version 2.3, the group ID element was added; the access right definition and health insurance module were amended.


Asunto(s)
Sistemas de Registros Médicos Computarizados/normas , Lenguajes de Programación , Programas Informáticos/normas , Humanos , Japón , Registro Médico Coordinado/normas
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