RESUMEN
Radioimmunoassay analyses of serum are widely performed to monitor the incidence of hepatitis B virus infection. As an alternative to serum analyses, the authors have explored the feasibility of using gingival crevicular fluid for the detection and quantitation of hepatitis B viral immunologic markers. The results of the study demonstrate that the hepatitis B surface antigen can be easily quantified in gingival fluid. Confirmation of the presence of the hepatitis B e antigen can also be made, but this is more difficult because of the small amounts of gingival fluid currently being assayed. Antibody to the hepatitis B surface antigen can be quantitated in high-titered persons.
Asunto(s)
Anticuerpos Antivirales/análisis , Antígenos Virales/análisis , Líquido del Surco Gingival/inmunología , Gingivitis/inmunología , Virus de la Hepatitis B/inmunología , Sangre , Portador Sano , Hepatitis B/inmunología , Humanos , RadioinmunoensayoRESUMEN
The ability of conjugates of peptides and 5-fluorocytosine or 5-fluoroorotic acid to enter Candida albicans was investigated. A number of conjugates of 5-fluoroorotic acid and peptides were synthesized using 1-(ethoxy-carbonyl)-2-ethoxy-1,2-dihydroquinoline as the coupling agent. Orotyl-L-leucyl-L-leucine, 5-fluoro-4-(N-succinamoyl-L-alanyl-L-leucine)-2(1H)-pyrimidinone [a 5-fluorocytosine derivative], and 5-fluoroorotyl-L-leucyl-L-leucine all inhibited the uptake of trimethionine into C. albicans WD 18-4. Inhibition by 5-fluoroorotyl-L-leucyl-L-leucine was competitive as judged using double-reciprocal plots. Evaluation of minimum inhibitory concentrations of peptide-5-fluorocytosine conjugates suggest that these conjugates enter C. albicans in the intact form. These results provide the first experimental evidence that peptides can carry pyrimidines into a eukaryote.