RESUMEN
The presence of Toxoplasma gondii DNA was detected in sera of acutely infected mice by polymerase chain reaction. Adult mice were inoculated intraperitoneally with 5 x 10(3) T. gondii RH strain tachyzoites. Five mice were killed every 3 hr from 3 to 21 hr post infection (PI) and every day from 1 to 7 days PI. Toxoplasma gondii DNA was first detected in 60% of the infected mice 18 hr PI and in 100% of the animals 21 hr PI and from 1 to 7 days PI. No mice survived longer than 7 days.
Asunto(s)
ADN Protozoario/sangre , Parasitemia/diagnóstico , Reacción en Cadena de la Polimerasa , Toxoplasma/aislamiento & purificación , Toxoplasmosis Animal/diagnóstico , Enfermedad Aguda , Animales , Electroforesis en Gel de Agar , Femenino , Ratones , Parasitemia/parasitología , Sensibilidad y Especificidad , Toxoplasma/genética , Toxoplasmosis Animal/parasitologíaRESUMEN
The beta-lactamases of six strains of Acinetobacter baumannii were investigated using analytical isoelectric focusing, and the MIC values for 16 beta-lactam antibiotics determined against the strains. Three strains produced a chromosomal cephalosporinase (pI > 8.5), one strain a CARB-5 beta-lactamase (pI = 6.35), one strain a TEM-1 penicillinase (pI = 5.4) as well as a cephalosporinase (pI > 8.5), and one carbapenem-resistant strain a beta-lactamase with a pI > 8.5. Ceftazidime, cefepime, cefpirome, imipenem and meropenem were found to be the most effective beta-lactams against five strains. Sulbactam was active against four of the strains at 1-4 mg/L and showed enhanced killing effects with cefpirome.
Asunto(s)
Acinetobacter/efectos de los fármacos , Antibacterianos/farmacología , Cefalosporinas/farmacología , Sulbactam/farmacología , Farmacorresistencia Microbiana , Pruebas de Sensibilidad Microbiana , beta-Lactamasas/metabolismoRESUMEN
The serum kinetics of vancomycin was studied in two patients aged 3 and 15 years during antibiotic therapy for catheter related sepsis associated with Staphylococcus epidermidis. Vancomycin was administered, simultaneously, by parenteral conventional doses (30 mg/kg/day div q 8 h) and using the antibiotic-lock technique in the infected catheter at a high concentration (150 mg/ml) during one hour, 3 hours after each infusion. Pharmacokinetics data did not show any significant change in the serum kinetics of the antibiotic. The results suggest that delivering a high concentration of vancomycin in the infected catheter using the lock technique may be useful to sterilize infected catheter without toxic effect.