RESUMEN
As the skin is the main protective organ of the body, it is exposed to wounds or injuries which carry out a healing process during a period of approximately 15 days depending on the severity of the injury. In the present research, the development of chitosan-based hydrogels loaded with silver nanoparticles and calendula extract (Ch-AgNPs-Ce) was proposed. This can be used to fulfill the hemostatic, anti-infective, antibacterial, healing and anti-inflammatory functions through controlled release of the nanoparticles and calendula extract in substitution of commonly used drugs. The physical properties of the silver nanoparticles were analyzed by UV-visible spectroscopy, scanning and transmission electron microscopy, showing a size between 50 and 100 nm. The antibacterial properties were evaluated by the agar well diffusion method. Antimicrobial testing of the hydrogels showed that the inclusion of silver nanoparticles provides concentration-dependent antibacterial behavior against E. coli and S. aureus. The healing properties of the system were tested in two diabetic patients to whom said hydrogels were placed, obtaining a positive curative result after a few weeks. Therefore, it can be concluded that Ch-AgNPs-Ce hydrogels can achieve healing in chronic or exposed wounds after a period of time which can be used in alternative treatments in patients with poor healing capacity.
Asunto(s)
Quitosano , Nanopartículas del Metal , Antibacterianos/farmacología , Antibacterianos/uso terapéutico , Calendula , Quitosano/farmacología , Preparaciones de Acción Retardada , Escherichia coli , Humanos , Hidrogeles/farmacología , Extractos Vegetales , Plata/farmacología , Staphylococcus aureus , Cicatrización de HeridasRESUMEN
Selenium (Se) is an essential micronutrient for diverse organisms such as mammals, bacteria, some insects and nematodes, archaea, and algae, as it is involved in a large number of physiological and metabolic processes and is part of approximately 25 selenoproteins in mammals. In plants, Se has no essential metabolic role, high concentrations of inorganic Se can lead to the formation of Se-amino acids, and its incorporation into selenoproteins can generate toxicity. Conversely, low doses of Se can trigger a variety of beneficial effects as an antioxidant, antimicrobial, or stress-modulating agent without being an essential element. Therefore, Se can generate toxicity depending on the dose and the chemical form in which it is supplied. Selenium nanoparticles (SeNPs) have emerged as an approach to reduce this negative effect and improve its biological properties. In turn, SeNPs have a wide range of potential advantages, making them an alternative for areas such as agriculture and food technology. This review focuses on the use of SeNPs and their different applications as antimicrobial agents, growth promoters, crop biofortification, and nutraceuticals in agriculture. In addition, the utilization of SeNPs in the generation of packaging with antioxidant and antimicrobial traits and Se enrichment of animal source foods for human consumption as part of food technology is addressed. Additionally, possible action mechanisms and potential adverse effects are discussed. The concentration, size, and synthesis method of SeNPs are determining factors of their biological properties.