RESUMEN

Background: Breast cancer shows significant clinical, morphologic, and molecular variation. Telomeres are nucleoprotein complexes composed of hexanucleotide repeat DNA sequence, TTAGGG, and numerous telomere-associated proteins. The maintenance of telomere length is carried out by a ribonucleoprotein called telomerase, which consists of two main components: a catalytic subunit called hTERT (human telomerase reverse transcriptase) and an RNA template called hTR (human telomerase RNA). The importance of evaluating hTERT expression lies in its potential therapeutic application, being an attractive target due to its almost non-existent expression in normal somatic cells. It is also expected that the anti-neoplastic effect would appear earlier in neoplastic cells with shorter telomeres. Additionally, a significant relationship has been observed between Her2-Neu overexpression and Her2-Neu positivity, which could suggest new combined therapies.The aim of this study was to detect the expression of hTERT, estrogen receptor (ER), progesterone receptor (PR), and HER2-Neu in neoplastic breast tissue embedded in paraffin before treatment and to investigate the relationship between them and with baseline and post-treatment telomere length, as well as with various clinicopathological parameters. Materials and methods: A cross-sectional-correlational, 21 women diagnosed with breast cancer at the Oncology Service of the High Specialty Medical Unit No. 1 of Bajio of the Mexican Institute of Social Security. The study complies with the Helsinki Declaration and was approved by the Institutional Ethical Committee of the Mexican Institute of Social Security (R-2019-1001-127). A peripheral blood sample was obtained before oncological treatment and at the end of oncological treatment for the measurement of telomere length by extracting DNA from leukocytes, was performed by the quantitative polymerase chain reaction (PCR) method described by Cawthon. Tumor samples were collected from each patient at the oncology department for immunohistochemical determination of biomarker expression (ER, PR, Her2/neu) and hTERT. Results: Of the 21 cases included in the study, the median age was 57.57 years. Eighteen cases were classified as invasive ductal carcinoma NOS (85.71%), 10 were histologic grade 2 (47.61%), 16 cases were hormone receptor positive (76.19%), 7 were Her2Neu positive (33.33%), and only 2 cases were triple negative (9.52%). Positive hTERT expression was detected in 11 cases (52.38%) and was negative in the remaining cases. A significant association was identified between hTERT-positive cases and Her2-Neu positive cases (p = 0.04). Baseline and post-treatment telomere lengths showed a significant difference using the non-parametric Wilcoxon t-test (p = 0.002). In hTERT-positive cases, there was significant telomere shortening at the end of oncological treatment (6.14 ± 1.54 vs. 4.75 ± 1.96 Kb, p = 0.007). Conclusion: Positive hTERT immunostaining cases were associated with poor prognostic factors, such as Her2-Neu overexpression and post-treatment telomere shortening. In the future, hTERT immunostaining could be used to select patients for therapies with antagonistic effects on hTERT, as well as in the selection of more appropriate chemotherapy regimens for patients who express it.

RESUMEN

The female reproductive tract microbiota is a complex community of microorganisms that might be crucial in maintaining a healthy reproductive environment. Imbalances in the bacterial community (dysbiosis) and the reduction of beneficial organisms and pathogen proliferation are associated with disease. Endometritis is a common cause of fertility problems in mares, and it is still challenging to diagnose and treat based on routine culture results of certain microorganisms. Although high-throughput sequencing studies provide helpful information regarding the composition of the reproductive tract microbiota in mares, there are still challenges in defining a "normal" microbiota. The primary objective of this literature review is to summarize the current knowledge regarding the microbiota present in the reproductive tract of mares, including the vagina, cervix, and uterus. The second objective is to describe the relevant factors that can impact the reproductive microbiota of mares, including the estrous cycle stage, the type of species (genera) investigated, season, and geographic location. The rationality of identifying the normal microbiota in the reproductive tract of a mare will likely aid in understanding the impact of the microbiota on the host's reproductive health and contribute to the treatment and prevention of equine sub and infertility issues.

RESUMEN

This descriptive cross-sectional study compared the microbiota of the uterus, vagina, clitoral fossa (CF), and perineal skin in healthy maiden mares during estrus. Twelve synchronized, healthy maiden mares (3-4 years old) from one single recipient mare herd were included. Microbial communities were characterized by amplifying the V3-V4 region of the 16S rRNA gene using the Illumina MiSeq platform. The uterine and vaginal microbiota had significantly lower richness (Chao-1) than the skin (p < 0.05). The uterine and vagina bacterial composition was similar in presence and abundance and could be differentiated from that of the CF and perineal skin. The microbial composition (Jaccard and Bray-Curtis distances) significantly differed across body-site locations (p < 0.05), which explained approximately 14% and 19% of the variation in microbial composition for Jaccard and Bray-Curtis distances, respectively. Firmicutes, Actinobacteria, Proteobacteria, and Bacteroidetes were the dominant taxa in the uterus and vagina, with higher proportions of Proteobacteria in the vaginal samples compared to the uterine samples. Streptococcaceae and Staphylococcaceae were present in high abundance in the uterine and vaginal samples, while Lactobacillaceae were not (<10%). We demonstrate that the uterine and vaginal microbiota of healthy maiden mares during estrus is similar but both distinct from that of the CF and perineal skin.

RESUMEN

We propose a distributed rewiring model which starts with a planar graph embedded into the Euclidean space and then behaves as a distributed system, where each node is provided with a set of dynamic links. The proposed rewiring evolves through cycles, where nodes explore the network to identify possible shortcuts and rewire their dynamic links. The rewiring decisions are subject to Euclidean and geodesic distance constrains. The emerging networks were assessed through topological and robustness analyses. We found that the networks display a variety of characteristics observed in complex networks encompassing phenomena such as preferential attachment, the distinctive traits of small-world networks, the presence of community structures, and robustness against degradation process. We consider that our proposal can be applied in the design of those self-managed systems in which there is a limitation on communication resources that can be represented by the Euclidean distance and, however, the components themselves can deploy strategies to optimize the transport of information and develop tolerance before contingencies.

RESUMEN

Despite the increasing interest in studying the gut mycobiota of dogs, the association between fungal colonization and the development of digestive disorders in this species remains largely understudied. On the other hand, the high prevalence of antifungal-resistant yeasts detected in previous studies in samples from animals represents a major threat to public health. We analyzed the presence of culturable yeasts in 112 rectal swab samples obtained from dogs with digestive disorders attended in a veterinary teaching hospital. Our results revealed that Malassezia pachydermatis was frequently isolated from the studied dog population (33.9% of samples), and that the isolation of this yeast was significantly associated to the age of animals, but not to their sex, disease group, or the presence of vomits and/or diarrhea. In contrast, other yeast species were less prevalent (17.9% of samples in total), and their isolation was not significantly associated to any variable included in the analysis. Additionally, we observed that 97.5% of the studied M. pachydermatis isolates (n = 158, 1-6 per positive episode) displayed a minimum inhibitory concentration (MIC) value >4 µg/ml to nystatin, 31.6% had a MIC ≥32 µg/ml to fluconazole, and 27.2% had a MIC >4 µg/ml to amphotericin B. The antifungal susceptibility profiles of non-Malassezia (n = 43, 1-7 per episode) were more variable and included elevated MIC values for some antifungal-species combinations. These results confirm that the intestine of dogs is a reservoir of opportunistic pathogenic yeasts and suggest that the prevalence of M. pachydermatis colonization depends more on the age of animals than on any specific digestive disorder.

Asunto(s)

RESUMEN

Objective: To improve susceptibility profiles of nosocomial bacteria, identifying the difference between infectious complications in patients undergoing endoscopic flexible ureterolithotomy (fURS) with negative urine culture (UC) that received extended antibiotic prophylaxis (EP) compared with standard antibiotic prophylaxis (SP). Methodology: This is a retrospective, observational, analytical cohort study, comparing infectious complications between patients undergoing fURS with negative UC who received EP versus SP. We include patients with susccessfull fURS, <20-mm stones and complete information. Results: Overall, 10.3% of patients had complications, 7.2% of patients had postoperative urinary infection, 1.8% had upper urinary tract infection (UTI) and 1.4% had urinary sepsis. Lower UTI were significantly more likely in the extended prophylaxis group with 6.8% versus 2.7% (RR = 2.8; 95% CI: 1.10-7.37, p = 0.030). The risk of upper UTI and sepsis did not show significant differences. A total of 69% patients with postoperative infection had isolated multidrug-resistant bacteria (MDRB) in the UC, with a higher risk in patients with extended prophylaxis (RR = 3.1; 95% CI: 1.33-7.59, p = 0.009). Conclusions: Patients with negative UC who underwent fURS using extended prophylaxis have two times higher risk of low UTI than patients with standard prophylaxis, without differences in the incidence of upper UTI or urinary sepsis. The risk of MDRB isolation in the postoperative UC is higher in the extended prophylaxis group, therefore we recommend the standard 60-min preoperative prophylaxis.

RESUMEN

The placenta is a temporary organ that is essential for the survival of the fetus, with a lifelong effect on the health of both the offspring and the dam. The functions of the placenta are controlled by its dynamic gene expression during gestation. In this study, we aimed to investigate the equine placental DNA methylome as one of the fundamental mechanisms that controls the gene expression dynamic. Chorioallantois samples from four (4M), six (6M), and ten (10M) months of gestation were used to map the methylation pattern of the placenta. Globally, methylation levels increased toward the end of gestation. We identified 921 differentially methylated regions (DMRs) between 4M and 6M, 1225 DMRs between 4M and 10M, and 1026 DMRs between 6M and 10M. A total of 817 genes carried DMRs comparing 4M and 6M, 978 comparing 4M and 10M, and 804 comparing 6M and 10M. We compared the transcriptomes between the samples and found 1381 differentially expressed genes (DEGs) when comparing 4M and 6M, 1428 DEGs between 4M and 10M, and 741 DEGs between 6M and 10M. Finally, we overlapped the DEGs and genes carrying DMRs (DMRs-DEGs). Genes exhibiting (a) higher expression, low methylation and (b) low expression, high methylation at different time points were identified. The majority of these DMRs-DEGs were located in introns (48.4%), promoters (25.8%), and exons (17.7%) and were involved in changes in the extracellular matrix; regulation of epithelial cell migration; vascularization; and regulation of minerals, glucose, and metabolites, among other factors. Overall, this is the first report highlighting the dynamics in the equine placenta methylome during normal pregnancy. The findings presented serve as a foundation for future studies on the impact of abnormal methylation on the outcomes of equine pregnancies.

Asunto(s)

RESUMEN

INTRODUCTION: Vaccines prevent disease and disability; save lives and represent a good assessment of health interventions. Several systematic reviews on the efficacy and effectiveness of COVID-19 vaccines have been published, but the immunogenicity and safety of these vaccines should also be addressed. AREAS COVERED: This systemic investigation sought to explain the efficacy, immunogenicity, and safety of new vaccination technologies against SARS-CoV-2 in people over 18 years old. Original research studying the effectiveness on mRNA, protein subunit vaccines, and viral vector vaccines against SARS-CoV-2 in people over 18 years old was analyzed. Several databases (Web of Science, Scopus, MEDLINE and EMBASE) were searched between 2012 and November 2022 for English-language papers using text and MeSH terms related to SARS-CoV-2, mechanism, protein subunit vaccine, viral vector, and mRNA. The protocol was registered on PROSPERO, CRD42022341952. Study quality was assessed using the NICE methodology. We looked at a total of six original articles. All studies gathered and presented quantitative data. EXPERT OPINION: Our results suggest that new vaccinations could have more than 90% efficacy against SARS-CoV-2, regardless of the technology used. Furthermore, adverse reactions go from mild to moderate, and good immunogenicity can be observed for all vaccine types.

Asunto(s)

RESUMEN

Transvaginal aspiration of oocytes (TVA) is a commonly used clinical procedure to obtain oocytes for in vitro production of embryos in horses. The goal of this study was to evaluate the effect of the TVA procedure on blood and peritoneal parameters, and to investigate the association of these findings with variables such as use of antibiotics, number of ovarian punctures, and length of the procedure. Physical examination was performed and blood and peritoneal fluid were obtained from 14 mares before they underwent TVA and the same parameters were assessed 24 hours after the procedure. On examination, 13/14 mares remained clinically healthy after the procedure. One mare developed low-grade fever, transient anorexia and mild colic that resolved within 12 hours post-TVA. The use of antibiotics, length of procedure and number of ovarian punctures did not have an effect on the measured parameters. All the mares presented significant changes in the leukogram, but these mostly remained within normal reference range values. The peritoneal parameters were also consistently affected after TVA. A significant post-TVA increase in lactate, total protein, and peritoneal neutrophil count was observed in peritoneal fluid. Peritoneal lactate level was elevated above baseline physiological levels in more than 50% of the mares. Results from this study indicate that there is an expected degree of inflammation after TVA procedures and peritoneal fluid parameters could be successfully used to monitor inflammation in the early stages.

Asunto(s)

RESUMEN

Wormlike micelles are long semiflexible cylindrical polymer structures formed by amphiphiles. In solution, these linear micelles percolate in multiconected entangled networks, where cross-links can break and recombine dynamically. Technological applications of wormlike micellar fluids include tunable encapsulation/delivery of molecules or colloids in biomedicine, oil industry, and/or cleaning processes. In this work, we propose that the experimental activation energy, the spatial confinement, and the mean first passage and escape times of a spherical tracer immersed in wormlike micellar network, in which caging effects are observed, can be estimated from economic Brownian dynamics simulations of a single particle interacting with an effective one-dimensional cosine-like potential of amplitudeU0and periodicityL. The proposed one-fitting parameter method has been used to characterize the long-time dynamics of wormlike micellar solutions formed by the self-assembly of a mixture of zwitterionic and anionic surfactants at several temperatures and different concentrations of surfactant and brine. The amplitudeU0has displayed a good agreement regarding the corresponding experimental activation energy at different temperatures. The periodicityLhas shown to be an upper bound of the mesh sizeξand of the same order of magnitude regarding the entanglement lengthle, obtained from rheology and microrheology experiments. The escape time of the tracer in the effective potentialτescapeand the timet*, at which a change of curvature in the mean square displacement occurs, are upper and lower limits, respectively, of the experimental relaxation time. Our method is simple and fast, and we foresee that it should be applicable to model the long-time behaviour of tracers in other polymer systems, in which caging effects are present.

RESUMEN

Nontuberculous mycobacteria (NTM) are ubiquitous microorganisms naturally resistant to antibiotics and disinfectants that can colonize drinking water supply systems. Information regarding the spread of NTM in specifically South America and Colombia is limited. We aimed to identify and characterize NTM present in tap water samples from Cali, Colombia. Drinking water samples and faucet biofilm swabs were collected in 18 places, including the city's three main water treatment plants (WTPs). Filter-trapped material and eluates (0.45 µm) from swab washes were plated in 7H11 agar plates. Suspected colonies were evaluated microscopically, and NTM species were identified based on the rpoB gene. Antibiotic susceptibility testing was also performed. Fifty percent (9/18) of sampling points were positive for NTM (including two WTPs), from which 16 different isolates were identified: Mycobacterium mucogenicum (8/16), M. phocaicum (3/16), M. chelonae (2/16), M. mageritense (2/16), and M. fortuitum (1/16), all rapidly growing mycobacteria. A susceptibility profile was obtained from 68.75% (11/16) of the isolates. M. chelonae was the most resistant species. All NTM isolated are potentially responsible for human diseases; our findings might provide a baseline for exploring NTM transmission dynamics and clinical characterization, as well as potential associations between NTM species found in drinking water and isolates from patients.

Asunto(s)

RESUMEN

The Enterobacter cloacae complex is an emerging opportunistic pathogen whose increased resistance to carbapenems is considered a public health problem. This is due to the loss of efficacy of beta-lactam antibiotics, which are used as the first treatment option in the management of infections caused by Gram-negative bacteria. The objective of this study was to perform the molecular characterization of 28 isolates of the E. cloacae complex resistant to cephalosporins and carbapenems isolated between 2011 and 2018 from five hospitals located in the municipality of Santiago de Cali, Colombia. Molecular detection of blaKPC, blaVIM, blaNDM and blaOXA-48-like genes was performed on these isolates and the genetic relationship between the isolates was assessed using multilocus sequence typing (MLST). Forty-three percent of the isolates carried the blaKPC-2 gene variant. MLST showed high genetic diversity among isolates, the most frequent being the sequence type ST510 with a frequency of 50%. The identification of the genes involved in carbapenem resistance and dispersing genotypes is an important step toward the development of effective prevention and epidemiological surveillance strategies in Colombian hospitals.

RESUMEN

Resumen Este artículo pretende identificar y analizar en la literatura la producción relacionada con la fatiga por compasión y el agotamiento psicológico presente en el personal sanitario que brinda atención y cuidado a personas durante los procesos de muerte y duelo de pacientes y sus familiares, respectivamente. Esta es una revisión integradora en las bases de datos JSTOR, ProQuest, Medline (vía PubMed), LILACS y Google Scholar en el periodo 2005-2018. La revisión permitió la identificación de tres cuerpos importantes de producción académica: trabajos de corte cualitativo que describen el fenómeno; los que desarrollan escalas y herramientas de medición del problema; y los que describen estrategias desarrolladas frente al mismo. En los contextos hospitalarios al personal de salud que acompaña a persona durante el proceso de muerte se le impide demostrar sentimientos y vivir el duelo por la pérdida de sus pacientes. Los trámites administrativos, la despersonalización sanitaria, el número de pacientes a cargo y la baja conciencia en relación con la muerte regulan de cierta forma lo acontecido, impidiendo que exista por parte del personal de salud descarga de sentimientos, llevándolos a presentar agotamiento psicológico y fatiga por compasión.

Abstract To identify and analyze in the literature the production related to comparison fatigue and psychological exhaustion present in healthcare personnel who provide care and attention to people during the death and grief processes of patients and their families, respectively. Integrative review in the JSTOR, ProQuest, Medline (vía PubMed), LILACS y Google Scholar databases in the period 2005-2018. The review identified the identification of three important bodies of academic production: on the one hand, qualitative studies that describe the phenomenon, secondly, those that scale and measurement tools of the problem and finally those that analyze strategies developed against the same. In hospital settings, health personnel accompanying a person during the death process are prevented from showing feelings and mourning the loss of their patients. The administrative procedures, the depersonalization of health, the number of patients in charge and the low conscience in relation to death regulated in a certain way, what happened, preventing health personnel from unloading feelings, leading them to present psychological exhaustion and fatigue. out of compassion.

Asunto(s)

RESUMEN

Placentitis has been reported as the most important cause of equine abortions, stillbirths, and perinatal deaths in horses. Most cases are caused by bacteria and less commonly by fungal elements. The aim of this report is to describe the clinical presentation of a fungal placentitis caused by Aspergillus terrerus. A 5-year-old thoroughbred maiden mare at the 217th day of gestation presented with some classic signs of placentitis (premature udder development and milk dripping). All ultrasonographic findings were consistent with a live fetus and a severe placentitis. On vaginal examination, purulent discharge was found coming from the external cervical os. Samples sent for culture yielded very small numbers of mixed growth including Enterococcus faecalis (by matrix-assisted laser desorption/ionization time-of-flight mass spectrometer), Streptococcus viridans, and Aspergillus terreus, and polymerase chain reaction was positive for Aspergillus terreus and Pseudomonas. The mare was placed on broad-spectrum antimicrobials, nonsteroidal anti-inflammatories, and hormonal and antifungal treatment. The fetus kept on developing and growing despite the placentitis for 14 days until the demise of the fetus in utero occurred. Aspergillus terreus was isolated from the chorionic surface but not from the fetus. Fungal placentitis is not very commonly found in mares. The extent of the placental lesions and the severity of the placentitis contributed to the death of the fetus. This is one of the few case reports available describing fungal placentitis. Aspergillus terreus has not been previously reported as a cause of placentitis.

Asunto(s)

RESUMEN

ABSTRACT Introduction: Patients with Down syndrome (DS) have an increased risk of developing autoimmune diseases. This is a rare case of a pediatric patient with DS with an initial clinical profile of diabetic ketoacidosis. Case presentation: 6-year-old male patient with symptoms suggestive of diabetes mellitus type 1 (DM1) of 15 days of evolution (polyuria, polydipsia, polyphagia and loss of 2 kilos of weight), who was admitted to the emergency department of the Hospital de San José, in Bogotá, Colombia, with uncontrollable vomiting and dehydration. The tests performed confirmed moderate ketoacidosis: glycometry: 592 mg/dL, pH: 7.19, HCO3: 10 mmol/L, PCO2: 45, PO2: 95 and lactic acid: 1.4 mmol/L. Management with isotonic fluids and intravenous insulin therapy was initiated and the patient was transferred to the pediatric intensive care unit, where ketoacidosis was controlled in approximately 10 hours. Subcutaneous insulin schedule was initiated without complications. Discussion: This case highlights the importance of monitoring possible autoimmune complications in patients with DS, since the risk of developing them is 4.2 times higher than in the general population. Conclusion: This case calls on to contemplate autoimmune complications in patients with DS during clinical practice. Although they are not part of the most frequent reasons for consultation, they cannot be underestimated and should be suspected and treated in a timely manner.

RESUMEN Introducción. Los pacientes con síndrome de Down (SD) tienen mayor riesgo de enfermedades autoinmunes. A continuación, se presenta un caso inusual de un paciente pediátrico con SD quien debuta con un cuadro de cetoacidosis diabética. Presentación del caso. Paciente masculino de 6 años con síntomas sugestivos de diabetes mellitus (DM) tipo 1 de 15 días de evolución (poliuria, polidipsia, polifagia y pérdida de 2 kilos de peso), quien ingresa al servicio de urgencias del Hospital de San José, en Bogotá, Colombia, con vómito incoercible y deshidratación. Se realizan exámenes que confirman cuadro de cetoacidosis moderada, glucometría: 592 mg/ dL, pH: 7.19, HCO3: 10 mmol/L, PCO2: 45, PO2: 95 y ácido láctico: 1.4 mmol/L. Se inicia manejo con líquidos isotónicos e insulinoterapia endovenosa y se traslada a la unidad de cuidado intensivo pediátrico, donde se controla la cetoacidosis en un aproximado de 1 0 horas. Se da inicio de esquema de insulina subcutáneo sin complicaciones. Discusión. Este caso resalta la importancia del seguimiento de posibles complicaciones autoinmunes en pacientes con SD, ya que el riesgo de estas es 4.2 veces mayor en población con SD. Conclusiones. El presente caso invita a contemplar las complicaciones autoinmunes en pacientes con SD durante la práctica clínica. Si bien no hacen parte de los motivos de consulta más frecuentes, no se pueden subestimar, sino que deben sospecharse y tratarse oportunamente.

RESUMEN

In this work, we propose that packets travelling across a wireless sensor network (WSN) can be seen as the active agents that make up a complex system, just like a bird flock or a fish school, for instance. From this perspective, the tools and models that have been developed to study this kind of systems have been applied. This is in order to create a distributed congestion control based on a set of simple rules programmed at the nodes of the WSN. Our results show that it is possible to adapt the carried traffic to the network capacity, even under stressing conditions. Also, the network performance shows a smooth degradation when the traffic goes beyond a threshold which is settled by the proposed self-organized control. In contrast, without any control, the network collapses before this threshold. The use of the proposed solution provides an effective strategy to address some of the common problems found in WSN deployment by providing a fair packet delivery. In addition, the network congestion is mitigated using adaptive traffic mechanisms based on a satisfaction parameter assessed by each packet which has impact on the global satisfaction of the traffic carried by the WSN.

Asunto(s)

RESUMEN

Con el objetivo de investigar el efecto del medio de cultivo en la productividad y tiempo de desarrollo huevo-adulto de una cepa silvestre y tres cepas mutantes (CyLv, vg, w) de Drosophila melanogaster, se examinaron dos tipos de medios: banano y naranja. Para esto se empleó un diseño con dos factores, medio de cultivo y tipo de cepa, para un total de ocho tratamientos con cinco repeticiones cada uno. Se obtuvo que la productividad y el tiempo de desarrollo dependen del medio de cultivo y el tipo de cepa, encontrándose mayor productividad en el medio de naranja. La cepa silvestre presentó la mayor productividad y el menor tiempo de desarrollo en los dos medios (α=0,05). El análisis genético evidenció una heredabilidad baja y una variación fenotípica debida en su mayor parte al componente de interacción genotipo-ambiente, lo que explica la diferencia en el patrón de productividad y tiempo de desarrollo entre medios de cultivo.

With the objective of investigating the effect of the culture media in the productivity and development time egg-adult of Drosophila melanogaster in +/+ and three mutants (CyLv, vg, w), two culture media: banana and orange, were evaluated. An experimental design with two factors: culture media and kind of flies-stock, were tested, for a total of eight treatments with five replicas each one. The productivity and development time depend on culture media and kind of flies-stock, and the biggest productivity was in the orange culture media. The +/+ presented the biggest productivity and lowest development time in both culture media (α=0.05). The genetic analysis showed a low heritability and the phenotypic variation was due, in a mayor part, to the component of the interaction genotype-environment that explains the difference in the patron of productivity and development time between culture media.