RESUMEN
Per- and polyfluoroalkyl substances (PFAS) are resistant to breakdown and are now considered ubiquitous and concerning contaminants. Although scientific and legislative interest in these compounds has greatly increased in recent decades, our knowledge about their environmental fate and their effects on organisms is still incomplete, especially those of the new generation PFAS. In this study, we analysed the level of PFAS contamination in the fish fauna of the Po River, the most important waterway in Italy, to evaluate the influence of different factors (such as fish ecological traits and parasitism) on the accumulation of 17 PFAS. After solvent extraction and purification, hepatic or intestinal tissues from forty specimens of bleak, channel catfish, and barbel were analysed by liquid chromatography coupled with mass spectrometry (LOQ = 2.5 ng/g w.w.). The prevalent PFAS were perfluorooctane sulfonate (PFOS), present in all samples at the highest concentration (reaching a maximum of 126.4 ng/g and 114.4 ng/g in bleak and channel catfish, respectively), and long-chain perfluoroalkyl carboxylic acids (PFDA and PFUnDA). Perfluorooctanoic acid and new generation PFAS (Gen X and C6O4) were not detected. Comparison of the hepatic contamination between the benthic channel catfish and the pelagic bleak showed similar concentrations of PFOS (p > 0.05) but significantly higher concentrations of other individual PFAS and of the sum of all measured PFAS (p < 0.05) in bleak. No correlation was found between the hepatic level of PFAS and fish size in channel catfish. For the first time, PFAS partitioning in a parasite-fish system was studied: intestinal acanthocephalans accumulated PFOS at lower levels than the intestinal tissue of their host (barbel), in contrast to what has been reported for other pollutants (e.g., metals). The infection state did not significantly alter the level of PFAS accumulation in fish, and acanthocephalans do not appear to be a good bioindicator of PFAS pollution.
Asunto(s)
Acantocéfalos , Ácidos Alcanesulfónicos , Fluorocarburos , Ictaluridae , Contaminantes Químicos del Agua , Animales , Ríos/química , Monitoreo del Ambiente/métodos , Contaminantes Químicos del Agua/análisis , Fluorocarburos/análisis , Ictaluridae/metabolismo , Ácidos Alcanesulfónicos/análisis , ItaliaRESUMEN
Despite the microbeads from cosmetic products do not contribute with high percentages to the microplastic pollution, they can pose a threat to the environment, being discharged into the water bodies and just partially blocked by the wastewater treatment plants. As environmental associations have been fighting for the abolition of microplastics in PCCPs, in many countries measures have been taken and bans are already operational or in process. Some cosmetic companies have voluntarily renounced the use of microbeads and some voluntary certifications of PCCPs prohibit their use. PCCPs recently analysed can contain levels of microbeads reaching 50,391 per g and can contribute with every single use to the introduction of 229,000 microbeads into the domestic sewage. Given the spread and danger of these pollutants, the problem is global and unthinkable to be solved by banning microplastic-containing products only in some countries, thus a general ban would be necessary.
Asunto(s)
Cosméticos/análisis , Contaminantes Ambientales/análisis , Plásticos/análisis , Monitoreo del Ambiente , InternacionalidadRESUMEN
This study investigated chromosomal aneuploidies and DNA damage in spermatozoa from male patients contaminated by perfluorinated compounds (PFCs) in whole blood and seminal plasma. Sperm aneuploidy and diploidy rate for chromosomes 18, X and Y were evaluated by FISH; sperm DNA fragmentation was assessed by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labelling technique coupled to flow cytometry. Our results indicated that PFC contamination was present in 58% of subjects included in the study. A significant increase in alterations of sperm parameters was observed in PFC-positive subjects compared to PFC-negative subjects. As regards the sperm aneuploidy, both disomy and diploidy rates resulted significantly increased in subjects positive for PFC contamination compared to PFC-negative samples. In addition, sperm DNA fragmentation index resulted significantly increased in PFC-contaminated subjects compared to PFC-non-contaminated subjects, with a significant increased level of dimmer DNA fragmentation index. Our results clearly indicate that PFC contamination may detrimentally affect spermatogenesis, disturbing both meiotic segregation and DNA integrity. We could therefore suggest cautions to reduce or eliminate any contact with these compounds because the long-term effects of PFC accumulation in the body are not predictable.
Asunto(s)
Ácidos Alcanesulfónicos/sangre , Aneuploidia , Astenozoospermia/metabolismo , Caprilatos/sangre , Aberraciones Cromosómicas/estadística & datos numéricos , Fragmentación del ADN , Fluorocarburos/sangre , Oligospermia/metabolismo , Espermatozoides , Adulto , Ácidos Alcanesulfónicos/metabolismo , Astenozoospermia/epidemiología , Astenozoospermia/genética , Caprilatos/metabolismo , Estudios de Casos y Controles , Cromatografía Líquida de Alta Presión , Cromosomas Humanos Par 18/genética , Cromosomas Humanos X/genética , Cromosomas Humanos Y/genética , Diploidia , Exposición a Riesgos Ambientales/estadística & datos numéricos , Citometría de Flujo , Fluorocarburos/metabolismo , Humanos , Hibridación Fluorescente in Situ , Etiquetado Corte-Fin in Situ , Italia/epidemiología , Masculino , Persona de Mediana Edad , Oligospermia/epidemiología , Oligospermia/genética , Semen/química , Recuento de Espermatozoides , Motilidad Espermática/genética , Espermatogénesis/genéticaRESUMEN
In 2003-2006, the distribution of macronutrients and pollutants of environmental interest was investigated in surficial sediments collected from 10 southern Italy harbors selected in four different regions. About 167 stations were sampled to determine levels of total organic carbon, total nitrogen, total phosphorous, trace elements (Al, Cd, Pb, Ni, Cr, Cu, Zn, Hg, As), short- and long-chain aliphatic hydrocarbons (Hy C > 12 and Hy C < 12), and concentrations of persistent organic pollutants (polychlorinated biphenyls, polycyclic aromatic hydrocarbons [PAHs], p-p-Dichlorodiphenyldichloroethylene (4,4'-DDE), and Hexachlorobenzene (HCB). General relationships between studied variables and harbors systems were explored by multivariate statistical approaches. Results show that wide fluctuations are reported for all variables both among harbors and inside each studied system. Principal components analysis suggests that major significance in explaining total average variability is due to lead, copper, zinc, silts, sands, and PAHs. No significance has been observed when testing nonmetric multidimensional scaling distributions relating with the factor "region," while performing analyses on factor "main human activity," a higher significance is observed. These results suggest a strong relationship between the main human use of marine systems and observed pollution levels in sediments.