RESUMEN
Arylamines and polycyclic aromatic hydrocarbons (PAHs) are hazardous anthropogenic pollutants in the environment. The toxicity of PAHs, which include benzo(α)pyrene (BP), is mediated by the activation of Ð 450 cytochromes of the 1Ð subfamily (CYP1A1 and CYP1A2). Previously, we have demonstrated that tocopherol, quercetin, and menadione inhibit the expression and activity of CYP1A in the liver of male Wistar rats after administration of a high BP dose to the rats for 3 days. Here, we confirmed the effects of tocopherol, quercetin, and menadione on the expression and activity of CYP1A and on rat liver morphology during prolonged administration (90 days) of a low BP dose. We revealed that subchronic oral administration of a low BP dose has no influence on CYP1A expression as compared to controls but can cause pathomorphological changes in rat liver tissue. These changes are abrogated by tocopherol, attenuated by quercetin, and enhanced by menadione.
Asunto(s)
Benzo(a)pireno , Citocromo P-450 CYP1A1 , Hígado , Quercetina , Tocoferoles , Vitamina K 3 , Animales , Benzo(a)pireno/toxicidad , Citocromo P-450 CYP1A1/genética , Hígado/efectos de los fármacos , Masculino , Hidrocarburos Policíclicos Aromáticos/toxicidad , Quercetina/farmacología , Ratas , Ratas Wistar , Tocoferoles/farmacología , Vitamina K 3/farmacologíaRESUMEN
Boron neutron capture therapy (BNCT) that is of the highest attractiveness due to its selective action directly on malignant tumor cells is a promising approach to treating cancers. Clinical interest in BNCT focuses in neuro-oncology on therapy for gliomas, glioblastoma in particular, and BNCT may be used in brain metastatic involvement. This needs an epithermal neutron source that complies with the requirements for BNCT, as well as a 10B-containing agent that will selectively accumulate in tumor tissue. The introduction of BNCT into clinical practice to treat patients with glial tumors will be able to enhance therapeutic efficiency.
Asunto(s)
Compuestos de Boro/uso terapéutico , Terapia por Captura de Neutrón de Boro , Neoplasias Encefálicas/radioterapia , Terapia por Captura de Neutrón de Boro/instrumentación , Terapia por Captura de Neutrón de Boro/métodos , Neoplasias Encefálicas/metabolismo , Humanos , Resultado del TratamientoRESUMEN
UNLABELLED: Currently one of the most promising approaches in development of cancer virotherapy is based on the ability of oncolytic viruses to selective infection and lysis of tumor cells. AIM: The goal of the study was to identify and evaluate perspective oncolytic viruses capable of selectively destroying human glioma cells. PATIENTS AND METHODS: Original GB2m, GA14m and GB22m glioma cell cultures derived from patients were used for evaluating in vitro oncolytic activity of some typical orthopoxviruses, adenoviruses and parvoviruses. RESULTS: The oncolytic activity in the human glioma cell models was confirmed for LIVP and WR strains of vaccinia virus, Adel2 and Ad2del strains with deletions within E1B/55K gene and derived from human adenoviruses type 2 and 5, respectively. CONCLUSIONS: We consider these oncolytic viruses as promising agents for the treatment of human malignant glioma.
Asunto(s)
Glioma , Viroterapia Oncolítica/métodos , Virus Oncolíticos/fisiología , Adenoviridae/fisiología , Técnicas de Cultivo de Célula , Glioma/terapia , Glioma/virología , Humanos , Orthopoxvirus/fisiología , Parvovirus/fisiología , Células Tumorales Cultivadas/virología , Fenómenos Fisiológicos de los VirusRESUMEN
Despite the advances of modern medicine, malignant glioblastoma cure remains an elusive goal. Both the invasive nature and location in vital areas of the brain make this type of tumors difficult for surgical treatment, while the current adjuvant therapy is not as successful as expected. Frequent recurrence and invasiveness of malignant gliomas is due to resistance of glioma stem cells to conventional radiation and chemotherapy. Technological advances in constructing recombinant viruses have allowed creating strains with high oncolytic activity toward glial tumors. Many of these strains have passed Phase I of clinical trials and demonstrated high safety. Despite the obvious potential of the approach, efficiency of the existing strains is still far from being sufficient for effectively curing the disease and require further improvement. The review summarizes results obtained with the most successful variants of oncolytic viruses that come down to the clinical trials and discusses the prospects for new approaches in virotherapy of malignant gliomas.
Asunto(s)
Neoplasias Encefálicas/terapia , Glioma/terapia , Viroterapia Oncolítica , Virus Oncolíticos , Animales , Neoplasias Encefálicas/genética , Neoplasias Encefálicas/metabolismo , Neoplasias Encefálicas/patología , Neoplasias Encefálicas/virología , Glioma/genética , Glioma/metabolismo , Glioma/patología , Glioma/virología , Humanos , Virus Oncolíticos/genética , Virus Oncolíticos/metabolismoRESUMEN
We studied in vitro effect of epithermal neutrons in various doses on viability of glioblastoma U87 tumor cells. Increasing the dose from 1.9 to 4.1 Sv promoted cell death. Cytofluorimetric analysis revealed no activation of apoptosis in the irradiated cells, which attested to necrotic death of the tumor cells exposed to epithermal neutron radiation.
Asunto(s)
Terapia por Captura de Neutrón de Boro , Supervivencia Celular/efectos de la radiación , Glioblastoma/radioterapia , Animales , Apoptosis/efectos de la radiación , Línea Celular Tumoral/efectos de la radiación , Forma de la Célula/efectos de la radiación , Fibroblastos/efectos de la radiación , Glioblastoma/patología , Humanos , Ratones , NeutronesRESUMEN
The function of the gene gilgamesh (89B9-12) encoding a casein kinase in Drosophila spermatogenesis was studied. The chimeric Gilgamesh-GFP protein in spermatocytes is cortically located. In the polar and apolar spermatocytes, it concentrates at the terminal ends of the fusome, the organelle that passes through the system of ring canals of the spermatocyte cyst. At the stage of spermatid elongation, the protein associates with the nucleus. A spot of the highest Gilgamesh-GFP concentration in the nucleus co-localizes with gamma-tubulin in the basal body. At later stages, Gilgamesh is localized to the individualization complex (IC), leaving the nuclei somewhat before the IC investment cones, as detected by actin binding. The sterile mutation due to the gilgamesh gene leads to the phenotype of scattered nuclei and altered structure of actin cones in the individualizing spermatid cyst. Ultrastructural evidence confirmed defective spermatid individualization due to the mutation. The phylogenetic origin of the protein, and the connection between vesicular trafficking and spermatid individualization, are discussed.
Asunto(s)
Quinasa de la Caseína I/metabolismo , Proteínas de Drosophila/metabolismo , Drosophila melanogaster/fisiología , Proteínas Fluorescentes Verdes/metabolismo , Proteínas Recombinantes de Fusión/metabolismo , Espermatogénesis/fisiología , Animales , Quinasa de la Caseína I/clasificación , Quinasa de la Caseína I/genética , Proteínas de Drosophila/clasificación , Proteínas de Drosophila/genética , Drosophila melanogaster/anatomía & histología , Células Germinativas/citología , Células Germinativas/metabolismo , Proteínas Fluorescentes Verdes/genética , Masculino , Filogenia , Proteínas Recombinantes de Fusión/genéticaRESUMEN
The cellular function of the gilgamesh mutation (89B9-12) of casein kinase gene in Drosophila spermatogenesis was studied. It was demonstrated that the sterility resulting from this mutation is connected with the abnormalities in spermatid individualization. A phylogenetic study of the protein sequences of casein kinases 1 from various organisms was conducted. The Gilgamesh protein was shown to be phylogenetically closer to the cytoplasmic casein kinase family, represented by the YCK3, YCK2, and YCK1 proteins of Saccharomyces cerevisiae and animal gamma-casein kinases. It is known that these yeast casein kinases are involved in vesicular trafficking, which, in turn, is related in its genetic control to the cell membrane remodeling during spermatid individualization. Thus, the data of phylogenetic analysis fit well the results obtained by studying the mutation phenotype.
Asunto(s)
Quinasa de la Caseína I/metabolismo , Proteínas de Drosophila/metabolismo , Filogenia , Espermátides/metabolismo , Espermatogénesis/fisiología , Animales , Quinasa de la Caseína I/genética , Proteínas de Drosophila/genética , Drosophila melanogaster , Infertilidad Masculina/genética , Infertilidad Masculina/metabolismo , Masculino , Mutación , Saccharomyces cerevisiae/enzimología , Saccharomyces cerevisiae/genética , Proteínas de Saccharomyces cerevisiae/genética , Proteínas de Saccharomyces cerevisiae/metabolismo , Espermátides/patologíaRESUMEN
In eukaryotic cells, mitotic events are controlled by evolutionarily conserved cyclin-dependent kinases (cdk): these kinases phosphorylate cell proteins, which causes structural reorganization of the entire cell. Our recent studies of Drosophila syncytial embryos have demonstrated that cdk1 activity is a key factor that controls nuclear pore complex assembly/disassembly and affects the structure of cytoplasmic pores in the annulate. In this paper, we report a comparative analysis of these cytoplasmic organelles throughout the cell-cycle and throughout the development of Drosophila syncytial embryos. Based on the results obtained, it was presupposed that distribution of annulate lamellae containing cytoplasmic pores could reflect the inactivation of the mitotic kinase cdk1 in Drosophila syncytial embryos.
Asunto(s)
Blastodermo/enzimología , Blastodermo/ultraestructura , Proteína Quinasa CDC2/metabolismo , Proteínas de Drosophila/metabolismo , Drosophila melanogaster/embriología , Mitosis , Animales , Citoplasma/enzimología , Citoplasma/ultraestructura , Drosophila melanogaster/enzimología , Drosophila melanogaster/ultraestructura , Retículo Endoplásmico/enzimología , Retículo Endoplásmico/ultraestructura , Microscopía Electrónica , Poro Nuclear/enzimología , Poro Nuclear/ultraestructuraRESUMEN
Higher-eukaryotic membrane compartmentalization of different processes provides the control of functional activity in cells. The nuclear envelope that consists of outer and inner nuclear membranes, lamina, and nuclear pore complexes is one of the most significant and complex cell compartments. It separates DNA replication and transcription in the nucleus from RNA translation in the cytoplasm and regulates the nuclear-cytoplasmic transport of different molecules. This review describes and discusses the structural organization and biochemical composition of different nuclear envelope components (except nuclear pore complexes, which were discussed in our previous review) as well as its dynamics during mitosis in vitro and in vivo. Special attention is given to the role of lamina in a group of human genetic diseases, collectively referred to as laminopathies.
Asunto(s)
Células Eucariotas/citología , Membrana Nuclear/fisiología , Membrana Nuclear/ultraestructura , Animales , Cordados/anatomía & histología , Drosophila/anatomía & histología , Células Eucariotas/metabolismo , Humanos , Lamina Tipo A/genética , Lipodistrofia Parcial Familiar/genética , Lipodistrofia Parcial Familiar/fisiopatología , Proteínas de la Membrana/genética , Mitosis , Distrofia Muscular de Emery-Dreifuss/genética , Distrofia Muscular de Emery-Dreifuss/fisiopatología , Mutación , Membrana Nuclear/química , Lámina Nuclear/genética , Lámina Nuclear/metabolismo , Proteínas Nucleares/genéticaRESUMEN
This review summarized data on the morphological and biochemical analysis of nuclear pore complexes, which are complex organelles providing the route of passive and active nuclear-cytoplasmic transport to different molecules in the eukaryotic cell. The morphology and functional role of nuclear pores in higher and lower eukaryotes, and molecular aspects of the import and export of molecules from the nucleus are described in addition to factors involved in the regulation of these process. Special attention has been paid to sequential steps of the nuclear pore assembly in vitro and in vivo.
Asunto(s)
Núcleo Celular/metabolismo , Células Eucariotas/fisiología , Poro Nuclear/fisiología , Poro Nuclear/ultraestructura , Animales , Transporte Biológico , Retículo Endoplásmico/metabolismo , Células Eucariotas/citología , Proteínas Fúngicas/metabolismo , Microscopía Electrónica , Mitosis , Poro Nuclear/metabolismo , Proteínas de Complejo Poro Nuclear/metabolismo , Levaduras/fisiologíaRESUMEN
This review is devoted to annulate lamellae, a specific compartment of endoplasmic reticulum that occurs, presumably, in actively growing and rapidly dividing cells (oocytes, embryonic and tumor cells). We summarized both earlier and recent data on the dustribution of annulate lamellae in various cell types, on their morphology, and the distribution of interaction with intracellular structures at various treatments. As the annulate lamellae contain cytoplasmic pore complexes, a special attention was paid to their relation with nuclear pores. Possible functions of the annulate lamellae in intracellular processes and, particularly, in nuclear envelope assembly, are discussed.