RESUMEN
Despite the significant amount of time spent in the domestic environment, culture-independent size distribution data of bioaerosols are largely missing. This study investigated the temporal changes in size-resolved bacterial aerosols in urban and semi-urban residential settings. Overall, airborne bacterial taxa identified in both sites were dispersed across particles of various sizes. qPCR analysis showed that outdoors bacteria dominated particles > 8 µm, whilst indoor bacterial loadings were greater with 1-2 µm (winter) and 2-4 µm (summer) ranges. Indoor and outdoor aerosols harboured distinct bacterial communities due to the dominance of human-associated taxa (Staphylococcus, Micrococcus, Corynebacterium) in indoor air. The aerosol microbiome exhibited significant temporal variation, with Actinobacteria, Gammaproteobacteria and Bacilli predominant indoors, whereas Actinobacteria, Alphaproteobacteria and Gammaproteobacteria were the most abundant taxa outdoors. The variation between the two residences was mostly driven by particles < 2 µm, whereas differences between indoors and outdoors were mostly influenced by particles > 2 µm. Source-tracking analysis estimated that household surfaces accounted for the greatest source proportion of bacteria, surpassing that of outdoor air, which varied due to natural ventilation throughout the year. Our findings provide new insights into the factors governing the aerosol microbiome in residential environments which are crucial for exposure assessment.
Asunto(s)
Aerosoles , Microbiología del Aire , Bacterias , Aerosoles/análisis , Bacterias/clasificación , Bacterias/genética , Bacterias/aislamiento & purificación , Humanos , Microbiota , Contaminación del Aire Interior/análisis , Monitoreo del Ambiente/métodos , Tamaño de la Partícula , Ciudades , Estaciones del Año , ViviendaRESUMEN
To date, few studies have examined the aerosol microbial content in Metro transportation systems. Here we characterised the aerosol microbial abundance, diversity and composition in the Athens underground railway system. PM10 filter samples were collected from the naturally ventilated Athens Metro Line 3 station "Nomismatokopio". Quantitative PCR of the 16S rRNA gene and high throughput amplicon sequencing of the 16S rRNA gene and internal transcribed spacer (ITS) region was performed on DNA extracted from PM10 samples. Results showed that, despite the bacterial abundance (mean = 2.82 × 105 16S rRNA genes/m3 of air) being, on average, higher during day-time and weekdays, compared to night-time and weekends, respectively, the differences were not statistically significant. The average PM10 mass concentration on the platform was 107 µg/m3. However, there was no significant correlation between 16S rRNA gene abundance and overall PM10 levels. The Athens Metro air microbiome was mostly dominated by bacterial and fungal taxa of environmental origin (e.g. Paracoccus, Sphingomonas, Cladosporium, Mycosphaerella, Antrodia) with a lower contribution of human commensal bacteria (e.g. Corynebacterium, Staphylococcus). This study highlights the importance of both outdoor air and commuters as sources in shaping aerosol microbial communities. To our knowledge, this is the first study to characterise the mycobiome diversity in the air of a Metro environment based on amplicon sequencing of the ITS region. In conclusion, this study presents the first microbial characterisation of PM10 in the Athens Metro, contributing to the growing body of microbiome exploration within urban transit networks. Moreover, this study shows the vulnerability of public transport to airborne disease transmission.