RESUMEN

To reduce the assay time for detecting virus-specific antibodies in serum, we developed microarray-based active immunoassay techniques for detecting West Nile virus (WNV)-specific IgM molecules in chicken blood. The assay uses electrophoretic concentration of IgM molecules onto WNV antigens arrayed on a dialysis membrane followed by detection of bound IgM molecules with functionalized magnetic beads as active labels. This assay takes only 15 minutes and has the same sensitivity as a commercially available human WNV IgM antibody-capture enzyme-linked immunosorbent assay (commonly called a MAC-ELISA) modified for use with chicken sera.

Asunto(s)

Pollos , Ensayo de Inmunoadsorción Enzimática/veterinaria , Enfermedades de las Aves de Corral/diagnóstico , Fiebre del Nilo Occidental/veterinaria , Virus del Nilo Occidental/inmunología , Animales , Anticuerpos Antivirales/análisis , Inmunoglobulina M/inmunología , Masculino , Enfermedades de las Aves de Corral/sangre , Enfermedades de las Aves de Corral/prevención & control , Valor Predictivo de las Pruebas , Juego de Reactivos para Diagnóstico/veterinaria , Sensibilidad y Especificidad , Fiebre del Nilo Occidental/diagnóstico