RESUMEN
BACKGROUND: Brain-derived neurotrophic factor (BDNF) influences neuron differentiation during development as well as the synaptic plasticity and neuron survival in adulthood. BDNF has been implicated in the pathogenesis of schizophrenia and depression. Val66Met polymorphism and BDNF serum level are potential biomarkers in neuropsychiatric disorders. The aim of this study was to determine the effect of BDNF gene Val66Met functional polymorphism on serum BDNF concentration in patients with schizophrenia, during depression episode and in healthy control group. METHODS: 183 participants were recruited (61 patients with depressive episode, 56 females with schizophrenia, 66 healthy controls) from Polish population. Serum BDNF levels were measured using ELISA method. Val66Met polymorphism was genotyped using PCR- RFLP method. RESULTS: Serum BDNF levels were not associated with Val66Met polymorphism in either of the groups. A significant increase of BDNF level in schizophrenia (pâ¯=â¯0.0005) and depression (pâ¯=â¯0.026) comparing to the control group has been observed. CONCLUSIONS: Our results suggest that the functional Val66Met BDNF polymorphism is not associated with BDNF serum levels, which is in line with previous findings. Replication studies on larger groups are needed.
Asunto(s)
Factor Neurotrófico Derivado del Encéfalo/sangre , Factor Neurotrófico Derivado del Encéfalo/genética , Depresión/sangre , Depresión/genética , Polimorfismo de Nucleótido Simple , Esquizofrenia/sangre , Esquizofrenia/genética , Adulto , Afecto , Estudios de Casos y Controles , Depresión/diagnóstico , Depresión/psicología , Femenino , Estudios de Asociación Genética , Marcadores Genéticos , Predisposición Genética a la Enfermedad , Humanos , Masculino , Persona de Mediana Edad , Fenotipo , Polonia , Esquizofrenia/diagnóstico , Psicología del EsquizofrénicoRESUMEN
Small bacterial RNAs (sRNAs) regulate translation by pairing with complementary sequences in their target mRNAs, in a process which is often dependent on the Hfq protein. Here, the secondary structure of a 95-nt long fragment of Salmonella fadL mRNA containing RybB sRNA binding site in the coding region was analyzed. The data indicated local rearrangements in this mRNA structure after the annealing of RybB. The filter retention data had shown that Hfq bound both RybB and the fadL mRNA fragment with tight affinities. Moreover, Hfq increased the rate of RybB annealing to fadL mRNA. These data indicate that Hfq directly participates in RybB interactions with the fadL mRNA.
Asunto(s)
Proteínas Bacterianas/genética , ARN Bacteriano/metabolismo , ARN Mensajero/metabolismo , ARN Pequeño no Traducido/genética , Salmonella/genética , Proteínas Bacterianas/metabolismo , Secuencia de Bases , Sitios de Unión , Secuencias Invertidas Repetidas , Conformación de Ácido Nucleico , ARN Bacteriano/genética , ARN Mensajero/genética , ARN Pequeño no Traducido/metabolismo , Salmonella/metabolismoRESUMEN
Schizophrenia has been associated with a large range of autoimmune diseases, with a history of any autoimmune disease being associated with a 45% increase in risk for the illness. The inflammatory system may trigger or modulate the course of schizophrenia through complex mechanisms influencing neurodevelopment, neuroplasticity and neurotransmission. In particular, increases or imbalance in cytokine before birth or during the early stages of life may affect neurodevelopment and produce vulnerability to the disease. A total of 27 polymorphisms of IL1N gene: rs1800587, rs17561; IL1B gene: rs1143634, rs1143643, rs16944, rs4848306, rs1143623, rs1143633, rs1143627; IL1RN gene: rs419598, rs315952, rs9005, rs4251961; IL6 gene: rs1800795, rs1800797; IL6R gene: rs4537545, rs4845617, rs2228145, IL10 gene: rs1800896, rs1800871, rs1800872, rs1800890, rs6676671; IL10RA gene: rs2229113, rs3135932; TGF1B gene: rs1800469, rs1800470; each selected on the basis of molecular evidence for functionality, were investigated in this study. Analysis was performed on a group of 621 patients with diagnosis of schizophrenia and 531 healthy controls in Polish population. An association of rs4848306 in IL1B gene, rs4251961 in IL1RN gene, rs2228145 and rs4537545 in IL6R with schizophrenia have been observed. rs6676671 in IL10 was associated with early age of onset. Strong linkage disequilibrium was observed between analyzed polymorphisms in each gene, except of IL10RA. We observed that haplotypes composed of rs4537545 and rs2228145 in IL6R gene were associated with schizophrenia. Analyses with family history of schizophrenia, other psychiatric disorders and alcohol abuse/dependence did not show any positive findings. Further studies on larger groups along with correlation with circulating protein levels are needed.
Asunto(s)
Proteína Antagonista del Receptor de Interleucina 1/genética , Interleucinas/genética , Polimorfismo de Nucleótido Simple , Receptores de Interleucina/genética , Esquizofrenia/genética , Factor de Crecimiento Transformador beta1/genética , Adulto , Familia , Femenino , Estudios de Asociación Genética , Predisposición Genética a la Enfermedad , Haplotipos , Humanos , Desequilibrio de Ligamiento , Masculino , Polonia , Población Blanca/genéticaRESUMEN
AIM: MMP-9 is a candidate gene related to the neurodevelopment hypothesis of schizophrenia. The aim of this research was TDT analysis of polymorphism -1562C>T MMP-9 gene in schizophrenia. METHODS: Research was carried out on 147 trios (patient and his/hers both healthy parents). Genetic material was isolated from leukocytes using the salting out method. Polymorphism was studied with PCR-RFLP, statistic analysis was made using transmission disquilibrium test by Haploview 4.2. RESULTS: There was no significant association between analyzed polymorphism of MMP-9 (-1562 C>T) and schizophrenia. CONCLUSIONS: Insignificant association doesn't exclude the possible contribution of MMP-9 to pathogenesis of schizophrenia. Further research is needed to be carried out on bigger groups and other populations.