RESUMEN

The long-term prognosis after surgical resection of canine insulinoma is poor. Signs of hypoglycaemia often recur soon after surgery because tumour tissue has only been resected partially and/or functional (micro-)metastases were present. Using quantitative real-time PCR, the expression of 16 target genes was compared between primary canine insulinomas and their corresponding metastases. There was significantly higher expression of genes encoding for growth hormone (GH) and insulin-like growth factor-1 (IGF-1) in metastases compared to their primary tumours. Immunohistochemical examination of proteins of the GH-IGF-1 axis revealed expression of GH, IGF-1 and GH receptor (GHR) in both primary insulinomas and metastases. Immunohistochemical staining for IGF-1 was significantly higher in metastases compared to primary tumours.

Asunto(s)

Enfermedades de los Perros/metabolismo , Regulación Neoplásica de la Expresión Génica/fisiología , Factor I del Crecimiento Similar a la Insulina/metabolismo , Insulinoma/veterinaria , Animales , Perros , Insulina/genética , Insulina/metabolismo , Factor I del Crecimiento Similar a la Insulina/genética , Insulinoma/metabolismo , Insulinoma/patología , Factor Inhibidor de Leucemia/genética , Factor Inhibidor de Leucemia/metabolismo , Reacción en Cadena en Tiempo Real de la Polimerasa , Receptores OSM-LIF/genética , Receptores OSM-LIF/metabolismo , Factores de Transcripción/genética , Factores de Transcripción/metabolismo