RESUMEN

A ribozyme gene directed at a specific cleavage of mRNA coding for PB1 protein, a component of RNA-dependent RNA-polymerase of influenza A virus, was constructed. The avian adenovirus CELO virus-associated RNA (VA RNA CELO) promoter and human cytomegalovirus (CMV) promoter were used for the permanent expression of the ribozyme in cell lines. The cells were infected with influenza A virus strains A/Singapore/1/57 and A/WSN/33, and the suppression of the virus reproduction and virus-specific protein synthesis was measured. The maximal level of the inhibition of virus reproduction as compared to the reproduction in non-transformed cells was 93.5%. Defective recombinant adenoviruses were constructed carrying the genes of functional and non-functional ribozymes under the control of human cytomegalovirus (CMV) promoter. The reproduction of A/WSN/33 virus in CV-1 cells preinfected with recombinant adenoviruses was shown to be suppressed.

Asunto(s)

Virus de la Influenza A/fisiología , ARN Catalítico/genética , ARN Catalítico/metabolismo , ARN Mensajero/metabolismo , ARN Polimerasa Dependiente del ARN/genética , Proteínas Virales/genética , Adenoviridae/genética , Animales , Secuencia de Bases , Línea Celular , Citomegalovirus/genética , Humanos , Virus de la Influenza A/genética , Datos de Secuencia Molecular , Plásmidos , Regiones Promotoras Genéticas , ARN Mensajero/genética , ARN Viral/genética , ARN Viral/metabolismo , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Transfección , Proteínas Virales/biosíntesis , Replicación Viral/fisiología