RESUMEN
Tissue morphogenesis requires dynamic intercellular contacts that are subsequently stabilized as tissues mature. The mechanisms governing these competing adhesive properties are not fully understood. Using gain- and loss-of-function approaches, we tested the role of p120-catenin (p120) and VE-cadherin (VE-cad) endocytosis in vascular development using mouse mutants that exhibit increased (VE-cadGGG/GGG) or decreased (VE-cadDEE/DEE) internalization. VE-cadGGG/GGG mutant mice exhibited reduced VE-cad-p120 binding, reduced VE-cad levels, microvascular hemorrhaging, and decreased survival. By contrast, VE-cadDEE/DEE mutants exhibited normal vascular permeability but displayed microvascular patterning defects. Interestingly, VE-cadDEE/DEE mutant mice did not require endothelial p120, demonstrating that p120 is dispensable in the context of a stabilized cadherin. In vitro, VE-cadDEE mutant cells displayed defects in polarization and cell migration that were rescued by uncoupling VE-cadDEE from actin. These results indicate that cadherin endocytosis coordinates cell polarity and migration cues through actin remodeling. Collectively, our results indicate that regulated cadherin endocytosis is essential for both dynamic cell movements and establishment of stable tissue architecture.
Asunto(s)
Antígenos CD/genética , Vasos Sanguíneos/crecimiento & desarrollo , Cadherinas/genética , Cateninas/genética , Desarrollo Embrionario/genética , Endotelio Vascular/crecimiento & desarrollo , Actinas/genética , Animales , Aorta/crecimiento & desarrollo , Aorta/metabolismo , Vasos Sanguíneos/metabolismo , Tipificación del Cuerpo/genética , Movimiento Celular/genética , Polaridad Celular/genética , Embrión de Mamíferos , Endocitosis/genética , Endotelio Vascular/metabolismo , Ratones , Unión Proteica/genética , Catenina deltaRESUMEN
RATIONALE: Endothelial barrier function depends on the proper localization and function of the adherens junction protein VE (vascular endothelial)-cadherin. Previous studies have suggested a functional relationship between integrin-mediated adhesion complexes and VE-cadherin yet the underlying molecular links are unclear. Binding of the cytoskeletal adaptor protein talin to the ß-integrin cytoplasmic domain is a key final step in regulating the affinity of integrins for extracellular ligands (activation) but the role of integrin activation in VE-cadherin mediated endothelial barrier function is unknown. OBJECTIVE: To test the requirement of talin-dependent activation of ß1 integrin in VE-cadherin organization and endothelial cell (EC) barrier function. METHODS AND RESULTS: EC-specific deletion of talin in adult mice resulted in impaired stability of intestinal microvascular blood vessels, hemorrhage, and death. Talin-deficient endothelium showed altered VE-cadherin organization at EC junctions in vivo. shRNA (short hairpin RNA)-mediated knockdown of talin1 expression in cultured ECs led to increased radial actin stress fibers, increased adherens junction width and increased endothelial monolayer permeability measured by electrical cell-substrate impedance sensing. Restoring ß1-integrin activation in talin-deficient cells with a ß1-integrin activating antibody normalized both VE-cadherin organization and EC barrier function. In addition, VE-cadherin organization was normalized by reexpression of talin or integrin activating talin head domain but not a talin head domain mutant that is selectively deficient in activating integrins. CONCLUSIONS: Talin-dependent activation of EC ß1-integrin stabilizes VE-cadherin at endothelial junctions and promotes endothelial barrier function.
Asunto(s)
Antígenos CD/fisiología , Cadherinas/fisiología , Células Endoteliales/fisiología , Integrina beta1/fisiología , Talina/fisiología , Animales , Antígenos CD/análisis , Cadherinas/análisis , Femenino , Células Endoteliales de la Vena Umbilical Humana/fisiología , Humanos , Uniones Intercelulares/metabolismo , Masculino , RatonesRESUMEN
Vascular endothelial (VE)-cadherin undergoes constitutive internalization driven by a unique endocytic motif that also serves as a p120-catenin (p120) binding site. p120 binding masks the motif, stabilizing the cadherin at cell junctions. This mechanism allows constitutive VE-cadherin endocytosis and recycling to contribute to adherens junction dynamics without resulting in junction disassembly. Here we identify an additional motif that drives VE-cadherin endocytosis and pathological junction disassembly associated with the endothelial-derived tumor Kaposi sarcoma. Human herpesvirus 8, which causes Kaposi sarcoma, expresses the MARCH family ubiquitin ligase K5. We report that K5 targets two membrane-proximal VE-cadherin lysine residues for ubiquitination, driving endocytosis and down-regulation of the cadherin. K5-induced VE-cadherin endocytosis does not require the constitutive endocytic motif. However, K5-induced VE-cadherin endocytosis is associated with displacement of p120 from the cadherin, and p120 protects VE-cadherin from K5. Thus multiple context-dependent signals drive VE-cadherin endocytosis, but p120 binding to the cadherin juxtamembrane domain acts as a master regulator guarding cadherin stability.
Asunto(s)
Cateninas/metabolismo , Proteínas Inmediatas-Precoces/metabolismo , Uniones Adherentes/metabolismo , Antígenos CD/metabolismo , Sitios de Unión , Cadherinas/metabolismo , Cateninas/genética , Cateninas/fisiología , Membrana Celular/metabolismo , Regulación hacia Abajo , Endocitosis , Células Endoteliales/metabolismo , Humanos , Proteínas Inmediatas-Precoces/fisiología , Ligasas , Fosfoproteínas/metabolismo , Cultivo Primario de Células , Unión Proteica , Proteolisis , Sarcoma de Kaposi , Ubiquitina/metabolismo , Ubiquitinación , Catenina deltaRESUMEN
Mammalian neuroepithelial stem cells divide using a polarized form of cytokinesis, which is not well understood. The cytokinetic furrow cleaves the cell by ingressing from basal to apical, forming the midbody at the apical membrane. The midbody mediates abscission by recruiting many factors, including the Kinesin-6 family member Kif20b. In developing embryos, Kif20b mRNA is most highly expressed in neural stem/progenitor cells. A loss-of-function mutant in Kif20b, magoo, was found in a forward genetic screen. magoo has a small cerebral cortex, with reduced production of progenitors and neurons, but preserved layering. In contrast to other microcephalic mouse mutants, mitosis and cleavage furrows of cortical stem cells appear normal in magoo. However, apical midbodies show changes in number, shape and positioning relative to the apical membrane. Interestingly, the disruption of abscission does not appear to result in binucleate cells, but in apoptosis. Thus, Kif20b is required for proper midbody organization and abscission in polarized cortical stem cells and has a crucial role in the regulation of cerebral cortex growth.
Asunto(s)
Corteza Cerebral/metabolismo , Citocinesis/fisiología , Cinesinas/metabolismo , Células-Madre Neurales/metabolismo , Animales , Polaridad Celular/genética , Expresión Génica , Cinesinas/genética , Ratones , Ratones Endogámicos C57BL , Microtúbulos/metabolismo , ARN Mensajero/biosíntesisRESUMEN
Proper development of axonal connections is essential for brain function. A forward genetic screen for mice with defects in thalamocortical development previously isolated a mutant called baffled. Here we describe the axonal defects of baffled in further detail and identify a point mutation in the Hspa5 gene, encoding the endoplasmic reticulum chaperone BiP/GRP78. This hypomorphic mutation of BiP disrupts proper development of the thalamocortical axon projection and other forebrain axon tracts, as well as cortical lamination. In baffled mutant brains, a reduced number of thalamic axons innervate the cortex by the time of birth. Thalamocortical and corticothalamic axons are delayed, overfasciculated, and disorganized along their pathway through the ventral telencephalon. Furthermore, dissociated mutant neurons show reduced axon extension in vitro. Together, these findings demonstrate a sensitive requirement for the endoplasmic reticulum chaperone BiP/GRP78 during axon outgrowth and pathfinding in the developing mammalian brain.
Asunto(s)
Axones/fisiología , Corteza Cerebral/anomalías , Proteínas de Choque Térmico/genética , Tálamo/anomalías , Animales , Células Cultivadas , Corteza Cerebral/citología , Corteza Cerebral/fisiología , Chaperón BiP del Retículo Endoplásmico , Femenino , Fibroblastos/citología , Pruebas Genéticas , Edad Gestacional , Masculino , Mamíferos , Ratones , Ratones Endogámicos C57BL , Ratones Mutantes Neurológicos , Vías Nerviosas/anomalías , Vías Nerviosas/citología , Vías Nerviosas/fisiología , Embarazo , Prosencéfalo/anomalías , Prosencéfalo/citología , Prosencéfalo/fisiología , Tálamo/citología , Tálamo/fisiologíaRESUMEN
Development of the mammalian inner ear requires coordination of cell proliferation, cell fate determination and morphogenetic movements. While significant progress has been made in identifying developmental signals required for inner ear formation, less is known about how distinct signals are coordinated by their downstream mediators. Members of the Rac family of small GTPases are known regulators of cytoskeletal remodeling and numerous other cellular processes. However, the function of Rac GTPases in otic development is largely unexplored. Here, we show that Rac1 and Rac3 redundantly regulate many aspects of inner ear morphogenesis. While no morphological defects were observed in Rac3(-/-) mice, Rac1(CKO); Rac3(-/-) double mutants displayed enhanced vestibular and cochlear malformations compared to Rac1(CKO) single mutants. Moreover, in Rac1(CKO); Rac3(-/-) mutants, we observed compromised E-cadherin-mediated cell adhesion, reduced cell proliferation and increased cell death in the early developing otocyst, leading to a decreased size and malformation of the membranous labyrinth. Finally, cochlear extension was severely disrupted in Rac1(CKO); Rac3(-/-) mutants, accompanied by a loss of epithelial cohesion and formation of ectopic sensory patches underneath the cochlear duct. The compartmentalized expression of otic patterning genes within the Rac1(CKO); Rac3(-/-) mutant otocyst was largely normal, however, indicating that Rac proteins regulate inner ear morphogenesis without affecting cell fate specification. Taken together, our results reveal an essential role for Rac GTPases in coordinating cell adhesion, cell proliferation, cell death and cell movements during otic development.
Asunto(s)
Oído Interno/embriología , Morfogénesis/genética , Neuropéptidos/metabolismo , Proteínas de Unión al GTP rac/metabolismo , Animales , Apoptosis/genética , Cadherinas/metabolismo , Adhesión Celular/genética , Proliferación Celular , Oído Interno/metabolismo , Oído Interno/patología , Galactósidos , Inmunohistoquímica , Hibridación in Situ , Indoles , Ratones , Ratones Noqueados , Microscopía Electrónica de Rastreo , Morfogénesis/fisiología , Neuropéptidos/genética , Proteínas de Unión al GTP rac/genética , Proteína de Unión al GTP rac1RESUMEN
Morphogenesis of sensory hair cells, in particular their mechanotransduction organelle, the stereociliary bundle, requires highly organized remodeling of the actin cytoskeleton. The roles of Rho family small GTPases during this process remain unknown. Here we show that deletion of Rac1 in the otic epithelium resulted in severe defects in cochlear epithelial morphogenesis. The mutant cochlea was severely shortened with a reduced number of auditory hair cells and cellular organization of the auditory sensory epithelium was abnormal. Rac1 mutant hair cells also displayed defects in planar cell polarity and morphogenesis of the stereociliary bundle, including bundle fragmentation or deformation, and mispositioning or absence of the kinocilium. We further demonstrate that a Rac-PAK (p21-activated kinase) signaling pathway mediates kinocilium-stereocilia interactions and is required for cohesion of the stereociliary bundle. Together, these results reveal a critical function of Rac1 in morphogenesis of the auditory sensory epithelium and stereociliary bundle.