RESUMEN
We screened the La Plata drinking water distribution network for fecal and total coliform bacterial indicator by purification procedures, cultivating 66 membrane-filtered samples from the two networks on m-T7 agar. Subterranean and river-derived water yielded 13 and 18 confirmed gram-negative bacillus isolates, with 54% and 72% representing total coliforms, respectively. Those from the former source were Klebsiella oxytoca, Enterobacter agglomerans, and Enterobacter aerogenes and from the latter Klebsiella oxytoca, Enterobacter agglomerans, and Enterobacter cloacae, genomic group 3. Since 58% of the samples were positive using m-T7 medium it is suggested that the inclusion in standard quality control protocols should be implemented.
Asunto(s)
Enterobacteriaceae/aislamiento & purificación , Microbiología del Agua , Contaminación del Agua/análisis , Abastecimiento de Agua/análisis , Algoritmos , Argentina , Técnicas de Tipificación Bacteriana , Técnicas Bacteriológicas , Medios de Cultivo/farmacología , Enterobacter/clasificación , Enterobacter/aislamiento & purificación , Enterobacteriaceae/efectos de los fármacos , Enterobacteriaceae/crecimiento & desarrollo , Klebsiella/aislamiento & purificación , Salud Urbana , Purificación del Agua/métodosRESUMEN
We screened the La Plata drinking water distribution network for fecal and total coliform bacterial indicator by purification procedures, cultivating 66 membrane-filtered samples from the two networks on m-T7 agar. Subterranean and river-derived water yielded 13 and 18 confirmed gram-negative bacillus isolates, with 54 and 72 representing total coliforms, respectively. Those from the former source were Klebsiella oxytoca, Enterobacter agglomerans, and Enterobacter aerogenes and from the latter Klebsiella oxytoca, Enterobacter agglomerans, and Enterobacter cloacae, genomic group 3. Since 58 of the samples were positive using m-T7 medium it is suggested that the inclusion in standard quality control protocols should be implemented.(AU)
Asunto(s)
Estudio Comparativo , Enterobacteriaceae/aislamiento & purificación , Microbiología del Agua , Contaminación del Agua/análisis , Abastecimiento de Agua/análisis , Algoritmos , Argentina , Técnicas de Tipificación Bacteriana , Técnicas Bacteriológicas , Medios de Cultivo/farmacología , Enterobacter/clasificación , Enterobacter/aislamiento & purificación , Enterobacteriaceae/efectos de los fármacos , Enterobacteriaceae/crecimiento & desarrollo , Klebsiella/aislamiento & purificación , Salud Urbana , Purificación del Agua/métodosRESUMEN
We screened the La Plata drinking water distribution network for fecal and total coliform bacterial indicator by purification procedures, cultivating 66 membrane-filtered samples from the two networks on m-T7 agar. Subterranean and river-derived water yielded 13 and 18 confirmed gram-negative bacillus isolates, with 54 and 72 representing total coliforms, respectively. Those from the former source were Klebsiella oxytoca, Enterobacter agglomerans, and Enterobacter aerogenes and from the latter Klebsiella oxytoca, Enterobacter agglomerans, and Enterobacter cloacae, genomic group 3. Since 58 of the samples were positive using m-T7 medium it is suggested that the inclusion in standard quality control protocols should be implemented.
Asunto(s)
Abastecimiento de Agua/análisis , Enterobacteriaceae , Contaminación del Agua/análisis , Microbiología del Agua , Algoritmos , Argentina , Técnicas de Tipificación Bacteriana , Técnicas Bacteriológicas , Medios de Cultivo , Enterobacter , Enterobacteriaceae , Klebsiella , Purificación del Agua/métodos , Salud UrbanaRESUMEN
We screened the La Plata drinking water distribution network for fecal and total coliform bacterial indicator by purification procedures, cultivating 66 membrane-filtered samples from the two networks on m-T7 agar. Subterranean and river-derived water yielded 13 and 18 confirmed gram-negative bacillus isolates, with 54
and 72
representing total coliforms, respectively. Those from the former source were Klebsiella oxytoca, Enterobacter agglomerans, and Enterobacter aerogenes and from the latter Klebsiella oxytoca, Enterobacter agglomerans, and Enterobacter cloacae, genomic group 3. Since 58
of the samples were positive using m-T7 medium it is suggested that the inclusion in standard quality control protocols should be implemented.
RESUMEN
La taxonomia bacteriana, basada tradicionalmente en descripciones de las caracteristicas de las celulas, ha comenzado a tener en cuenta los test de acidos nucleicos para establecer las relaciones entre distintas especies. Mas recientemente, se ha enfatizado el estudio del ARN de los ribosomas. Se describen los metodos utilizados para tales estudios
Asunto(s)
Bacterias , Microbiología , Genética , BacteriologíaRESUMEN
A total of 173 Vibrio cholerae O1 isolates from the recent cholera epidemic in Colombia was analysed by the polymerase chain reaction (PCR) for the genes encoding the A subunit of cholera toxin (ctxA) and the zonula occludens toxin (zot), and by ribotyping. All isolates were positive for ctx A and zot, which was confirmed by hybridisation. Ribotyping with restriction endonuclease Bg/I digestion of total DNA revealed three ribotypes: B5a comprising 165 (96.4%) isolates, and two new designated ribotypes B20 and B21a in six (3.5%) isolates and two (1.1%) isolates, respectively. These findings have significant public health implications.
Asunto(s)
Toxina del Cólera/genética , Cólera/epidemiología , ARN Ribosómico/genética , Vibrio cholerae/genética , Técnicas de Tipificación Bacteriana , Cólera/microbiología , Colombia/epidemiología , Brotes de Enfermedades , Endotoxinas , Genes Bacterianos , Humanos , Epidemiología Molecular , Reacción en Cadena de la Polimerasa , Polimorfismo de Longitud del Fragmento de Restricción , ARN Bacteriano/genética , Vibrio cholerae/clasificación , Vibrio cholerae/aislamiento & purificación , Operón de ARNrRESUMEN
The rRNA gene restriction patterns of 92 isolates of Haemophilus influenzae biogroup aegyptius, associated with conjunctivitis or Brazilian purpuric fever in the State of São Paulo, Brazil, were studied with 16 + 23S rRNA from Escherichia coli as a probe. All strains were classified into 15 patterns. Isolates from Brazilian purpuric fever cases were seen only in patterns 3 (most frequently) and 4 (rarely), whereas isolates from conjunctivitis were found in all 15 patterns. The study demonstrated that rRNA from E. coli can serve as a probe for molecular epidemiology.