RESUMEN
Mesenchymal stromal cells (MSC) are considered to be promising tool of regenerative medicine. Migration of MSC toward damaged inflammatory site is essential for physiological tissue reparation. Therefore we studied modifications of migratory features of adipose tissue derived MSC (AT-MSC) after co-cultivation with activated monocytes derived from THP-1 cell line. As a result, we have observed an increased migration rate of AT-MSC in vitro in the absence of chemoattractant gradient as well as toward the gradient of PDGF BB (platelet-derived growth factor BB), which is well known chemoattractant for the cells of mesenchymal origin. Furthermore, the rate of directional AT-MSC migration through fibronectin was also increased. We have established that signaling from PDGFRß which is activated through binding of integrin receptors with extracellular matrix may be possible way to stimulate cellular migration under simulated inflammatory conditions.
Asunto(s)
Quimiotaxis/efectos de los fármacos , Matriz Extracelular/genética , Células Madre Mesenquimatosas/efectos de los fármacos , Monocitos/efectos de los fármacos , Proteínas Proto-Oncogénicas c-sis/farmacología , Receptor beta de Factor de Crecimiento Derivado de Plaquetas/genética , Tejido Adiposo/citología , Tejido Adiposo/metabolismo , Adulto , Becaplermina , Diferenciación Celular , Línea Celular , Células Cultivadas , Técnicas de Cocultivo , Matriz Extracelular/metabolismo , Expresión Génica , Humanos , Cadenas alfa de Integrinas/genética , Cadenas alfa de Integrinas/metabolismo , Cadenas beta de Integrinas/genética , Cadenas beta de Integrinas/metabolismo , Células Madre Mesenquimatosas/citología , Células Madre Mesenquimatosas/metabolismo , Persona de Mediana Edad , Monocitos/citología , Monocitos/metabolismo , Unión Proteica , Receptor beta de Factor de Crecimiento Derivado de Plaquetas/agonistas , Receptor beta de Factor de Crecimiento Derivado de Plaquetas/metabolismo , Transducción de Señal , Acetato de Tetradecanoilforbol/farmacologíaRESUMEN
Retrospective comparative analysis of therapy of the rheumatoid arthritis was undertaken. The following variants of antiphlogistic prescription: inactive tactics (long-lasting administration of one medicine), tactics of protracted treatment (separate courses of disease-modifying antirheumatic drugs with long breaks between them), tactics of first aid (at the beginning "passive treatments", after the appearance of complications begun active therapy with cytostatics); tactics of pyramid (the order of the prescription of medicines corresponds to classical strategy of baseline therapy with short breaks during the treatment), tactics of active therapy (the early beginning of baseline therapy with consistent change of medicine); effectiveness and safety of long-lasting administration of antiphlogistic in complex therapy of the rheumatoid arthritis.
Asunto(s)
Antirreumáticos/administración & dosificación , Artritis Reumatoide/tratamiento farmacológico , Citostáticos/administración & dosificación , Adulto , Artritis Reumatoide/diagnóstico , Femenino , Humanos , Masculino , Persona de Mediana EdadRESUMEN
By the example of rheumatoid arthritis was considered the methods of economic assessment of advisability of modern biological medication usage in military medicine. The usage of these medications leads to quick clinical response, decrease of the additional medication intake, decrease of the average duration of treatment, "move aside" time of permanent disablement. Modern active therapy can increase the effectiveness of treatment; improve the quality of life, decrease the average duration of hospital stay, following treatment expense and welfare payment for this category of ill.