RESUMEN

The fluorescent dye fura-2 AM was employed to record activation of Ca2+ entry in response to a decrease in Ca2+ concentration in the endoplasmic reticulum. Using whole-cell voltage clamp technique, we revealed Ca2+ currents with an amplitude of 0.46±0.13 pA/pF that passed through selective channels with current-voltage characteristics similar to those of classical store-operated CRAC channels. These currents were sensitive to 2-APB (50 µM), an inhibitor of store-operated channels. The data suggest that store-operated calcium entry is a characteristic feature of mature ventricular cardiomyocytes. Pathological alterations in store-operated Ca2+ entry can be implicated in the development of heart diseases.

Asunto(s)

Canales de Calcio Activados por la Liberación de Calcio/fisiología , Señalización del Calcio/fisiología , Calcio/metabolismo , Retículo Endoplásmico/metabolismo , Transporte Iónico/fisiología , Miocitos Cardíacos/fisiología , Animales , Células Cultivadas , Fura-2/análogos & derivados , Fura-2/farmacología , Ventrículos Cardíacos/metabolismo , Ratones , Miocitos Cardíacos/metabolismo , Técnicas de Placa-Clamp , Función Ventricular