RESUMEN

AIM: To evaluate gastrointestinal sequelae and growth impairment at school age in children who suffered from necrotising enterocolitis (NEC). METHODS: This historic cohort study compared all surviving children born in Denmark between 1 January 2002 and 31 December 2011 with NEC in the newborn period, to surviving children without NEC, but same gestational age, birthweight and year of birth. Outcomes were investigated through a parental questionnaire, including gastrointestinal and growth-related outcomes. We performed exploratory ad hoc analysis, by adjusting for possible confounding and by dividing NEC children into surgical and medical. RESULTS: In total, 163 children with NEC (50%) and 237 (36%) without NEC completed the parental questionnaire. Episodes of diarrhoea were more often reported in the NEC group (p = 0.0002). The increased risk seemed to be limited to those who underwent surgery for NEC. The absence from school (1.67 versus 1.31 days), rate of low height for age (17.9 versus 12.1%) and weight (29.9 versus 31.6 kg) did not differ significantly between children with NEC and children without NEC. CONCLUSION: Our findings suggest that long-term gastrointestinal complications following NEC appeared to be of little clinical importance at the population level and therefore do not encourage specific routine follow-up.

Asunto(s)

Desarrollo Infantil , Enterocolitis Necrotizante/epidemiología , Adolescente , Niño , Dinamarca/epidemiología , Diarrea/epidemiología , Femenino , Estudios de Seguimiento , Humanos , Recién Nacido , Masculino

RESUMEN

OBJECTIVE: The aim of the study is to compare data on the examined population of informal caregivers of people suffering from dementia with previous studies, as well as to assess the correlation between (i) depression determined on the basis of the Center for Epidemiologic Studies Depression Scale and (ii) caregiver burden measured by means of the Zarit Caregiver Burden Scale and some chosen parameters, such as total time devoted to caregiving, time of caregiving in hours per week and level of dementia severity measured by Global Deterioration Scale. PATIENTS AND METHODS: 41 informal caregivers of people suffering from dementia from different backgrounds were evaluated using the Zarit Caregiver Burden Scale and the Center for Epidemiologic Studies Depression Scale. Demographic data about the time devoted to caregiving and the number of hours spend on caregiving weekly were gathered. The type of dementia and its stage were registered using the Global Deterioration Scale (GDS). With the aid of the Statistica StatSoft program, mutual correlations between the parameters were measured. The study was conducted within the framework of AAL UnderstAID--a platform that supports and helps to understand and assist caregivers in the care of a relative with dementia. The international project is co-founded by the Joint Programme Ambient Assisted Living (Grant code: ESR-aal 2012 5 107). RESULTS: No significant correlations between the level of depression severity evaluated in caregivers and the total time of taking care of a demented person or time of caregiving in hours per week were observed. Similarly, no significant correlation between depression severity level and dementia severity level measured on the GDS scale were noted. There was also no significant correlation between Zarit Caregiver Burden Scale scores and the above-mentioned parameters. CONCLUSIONS: The level of depression among caregivers do not depend on socio-demographic factors.

Asunto(s)

Cuidadores/psicología , Demencia/terapia , Depresión , Adulto , Anciano , Anciano de 80 o más Años , Demografía , Depresión/etiología , Femenino , Humanos , Masculino , Persona de Mediana Edad , Autocuidado/psicología , Encuestas y Cuestionarios , Factores de Tiempo

RESUMEN

In the present investigation 20,000 broiler parents were vaccinated during rearing with Nobilis Escherichia coli vaccine and were placed in two out of four identical houses, with the remaining two houses on the same farm accommodating 20,000 unvaccinated control birds. During the production period a total of 335 dead birds (including 171 vaccinated and 164 control birds) randomly selected from the four houses were subjected to post-mortem examination. Although the overall mortality between the vaccinated and control flocks did not differ, mortality due to E. coli infections made up only 8.2% in vaccinated birds compared with 24.6% in unvaccinated birds. All E. coli isolates recovered from internal organs were assigned to the same phylogenetic group (B2), but a major genetic diversity was outlined by multilocus sequence typing. Only a single isolate was demonstrated to harbour a gene encoding the P-fimbriae variant F11, a key component of the Nobilis vaccine. Significant differences in average first week mortality, calculated average weight at 38 days and food conversion rate among broiler flocks originating from vaccinated and control birds, respectively, were not found. Further investigations are needed to explain the protection observed and the impact on the genetic diversity of E. coli.

Asunto(s)

Pollos , Infecciones por Escherichia coli/veterinaria , Vacunas contra Escherichia coli/inmunología , Escherichia coli/genética , Variación Genética , Enfermedades de las Aves de Corral/microbiología , Enfermedades de las Aves de Corral/mortalidad , Enfermedades de las Aves de Corral/prevención & control , Animales , Peso Corporal , Análisis por Conglomerados , Cartilla de ADN/genética , Escherichia coli/inmunología , Infecciones por Escherichia coli/mortalidad , Infecciones por Escherichia coli/prevención & control , Filogenia , Análisis de Secuencia de ADN

RESUMEN

AIMS: The aim of the present investigation was to identify and characterize Pasteurella-like isolates obtained from clinically affected psittacine birds. METHODS AND RESULTS: A total of 37 isolates from psittacine birds tentatively classified with the family Pasteurellaceae were characterized phenotypically. The genetic relationship was investigated by sequencing of partial rpoB and 16S rRNA genes for selected isolates. The results obtained were compared with the data from 16 reference strains. Nine isolates were identified as Gallibacterium spp., 16 as Volucribacter spp. or Volucribacter-like, while 11 isolates were classified as taxon 44 of Bisgaard. A single isolate was identified as Pasteurella multocida. CONCLUSIONS: Characterization of Pasteurellaceae by traditional methods is often inconclusive because of inconsistent reactions and phenotypic diversity. For the same reason, genotyping is essential to allow proper classification as demonstrated in the present study. SIGNIFICANCE AND IMPACT OF THE STUDY: Limited information exists on the isolation and significance of Pasteurellaceae associated with clinically affected psittacine birds showing signs of digestive and/or respiratory disorders. The present investigations demonstrated that these organisms are widely distributed among clinically affected birds, but isolation of these taxa cannot be unambiguously correlated with the symptoms observed.

Asunto(s)

Enfermedades de las Aves/microbiología , Infecciones por Pasteurella/veterinaria , Pasteurellaceae/clasificación , Pasteurellaceae/aislamiento & purificación , Psittaciformes/microbiología , Animales , Proteínas Bacterianas/genética , Datos de Secuencia Molecular , Infecciones por Pasteurella/microbiología , Pasteurellaceae/genética , Fenotipo , Filogenia , ARN Ribosómico 16S/genética

RESUMEN

Although oligodendrocytes are vulnerable to focal cerebral ischemia, remyelination of denuded or regenerating axons in the peri-infarct area has been observed in the central nervous system. We studied the expression of myelin basic protein (MBP), a major component of central nervous system myelin, in peri-infarct areas in adult rat brain after transient middle cerebral artery occlusion (MCAO) and correlated it to the expression of the growth-associated protein-43 (GAP-43), a marker for axonal regeneration and sprouting, using non-radioactive in situ hybridization techniques. Within the infarct, MBP messenger RNA (mRNA) had disappeared by 24 h, whereas myelin protein, identified by MBP and myelin oligodendrocyte glycoprotein (MOG) immunohistochemistry, appeared structurally intact until day 3. Peri-infarct oligodendrocytes increased their expression of MBP mRNA from 24 h to maximal levels at day 7, corresponding to the appearance of process-bearing MBP and occasional MOG-immunoreactive oligodendrocytes in parallel sections. Quantitative analysis revealed significant increases in the density of oligodendrocytes (up to 7.6-fold) and in the level of MBP mRNA expressed by individual cells. Parallel sections showed that increased expression of GAP-43 mRNA in neurons was concomitant to MBP mRNA upregulation in oligodendrocytes. While the mechanisms regulating oligodendrocyte survival and myelination signals are not clear at this point, axonal sprouting could putatively serve as a stimulus for the upregulation of oligodendrocyte cell numbers, differentiation state, and/or active myelination in the peri-infarct areas.

Asunto(s)

Isquemia Encefálica/metabolismo , Encéfalo/metabolismo , Infarto Cerebral/metabolismo , Proteína GAP-43/metabolismo , Proteína Básica de Mielina/metabolismo , ARN Mensajero/metabolismo , Regulación hacia Arriba/genética , Animales , Encéfalo/patología , Encéfalo/fisiopatología , Isquemia Encefálica/patología , Isquemia Encefálica/fisiopatología , Infarto Cerebral/patología , Infarto Cerebral/fisiopatología , Proteína GAP-43/genética , Regulación de la Expresión Génica/fisiología , Inmunohistoquímica , Hibridación in Situ , Masculino , Proteína Básica de Mielina/genética , Vaina de Mielina/metabolismo , Vaina de Mielina/patología , Degeneración Nerviosa/genética , Degeneración Nerviosa/metabolismo , Degeneración Nerviosa/patología , Ratas , Ratas Endogámicas SHR , Transcripción Genética/fisiología

RESUMEN

We present here sensitive, simple and robust methods for detection of tumor necrosis factor (TNF) mRNA and TNF in histological sections and homogenates of brain tissue from mice subjected to focal cerebral ischemia or hippocampal axonal lesioning. Both types of lesions are characterized by induction of TNF synthesis in resident microglial cells, which in the ischemic lesions are supplemented by TNF synthesizing, blood-borne macrophages. In situ hybridization for TNF mRNA is performed using alkaline phosphatase-labelled oligodeoxynucleotide probes. These probes show excellent rendition of individual cells, and can successfully be combined with immunohistochemical procedures. We also describe a sensitive immunohistochemical method for detection of TNF, which can be combined with visualization of an additional antigen. The specificity of the histological procedures are confirmed by RT-PCR and Western blot analysis on homogenates prepared from microdissected brain regions. Advantages and disadvantages of the methods are discussed with emphasis on the specificity and sensitivity of the histological procedures. Our strategy for detection of TNF mRNA and protein provides a solid basis for clarifying the cellular synthesis, regulation and function of TNF in the normal, injured or diseased CNS. Furthermore, the methodology can readily be applied in studies of other cytokines and growth factors in the CNS.

Asunto(s)

Corteza Cerebral/química , Inmunohistoquímica/métodos , Hibridación in Situ/métodos , Factor de Necrosis Tumoral alfa/análisis , Factor de Necrosis Tumoral alfa/genética , Animales , Western Blotting , Corteza Cerebral/patología , Corteza Cerebral/fisiopatología , Cartilla de ADN , Femenino , Proteína Ácida Fibrilar de la Glía/análisis , Infarto de la Arteria Cerebral Media/patología , Infarto de la Arteria Cerebral Media/fisiopatología , Antígeno de Macrófago-1/análisis , Masculino , Ratones , Ratones Endogámicos , Microglía/química , Vía Perforante/patología , Vía Perforante/fisiopatología , Vía Perforante/cirugía , ARN Mensajero/análisis , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Sensibilidad y Especificidad

RESUMEN

The proinflammatory cytokine tumor necrosis factor (TNF) is known to be expressed in brain ischemia; however, its cellular and temporal appearance is not fully settled. In this study, nonradioactive in situ hybridization for murine TNF mRNA was performed on brain sections from adult C57x129 mice at 6 hours, 12 hours, 24 hours, 2 days, 5 days, or 10 days (six to eight mice per group) after induction of permanent focal cerebral ischemia. Cortical infarct volumes were estimated, and TNF mRNA-expressing cells were counted within the infarct and infarct border using Cast-Grid analysis. At 12 hours, a peak of 19.2 +/- 5.1 TNF mRNA-expressing cells/mm2 was counted, contrasting two to three times lower values at 6 and 24 hours (6.4 +/- 4.6 and 9.2 +/- 3.4 cells/mm2, respectively) and <2 cells/mm2 at 48 hours and later stages. The TNF mRNA-expressing cells were distributed along the entire rostrocaudal axis of the cortical infarcts and occasionally within the caudate putamen. At all time points, TNF mRNA colocalized with Mac-1-positive microglia/macrophages but not with Ly-6G (Gr-1)-positive polymorphonuclear leukocytes. Similarly, combined in situ hybridization for TNF mRNA and immunohistochemistry for glial fibrillary acidic protein at 12 and 24 hours revealed no TNF mRNA-expressing astrocytes at these time points. Translation of TNF mRNA into bioactive protein was demonstrated in the neocortex of C57B1/6 mice subjected to permanent middle cerebral artery occlusion. In summary, this study points to a time-restricted microglial/macrophage production of TNF in focal cerebral ischemia in mice.

Asunto(s)

Arteriopatías Oclusivas/metabolismo , Arterias Cerebrales , Macrófagos/metabolismo , Microglía/metabolismo , Factor de Necrosis Tumoral alfa/metabolismo , Animales , Arteriopatías Oclusivas/complicaciones , Isquemia Encefálica/complicaciones , Infarto Cerebral/etiología , Infarto Cerebral/metabolismo , Infarto Cerebral/patología , Antígeno de Macrófago-1/metabolismo , Masculino , Ratones , Ratones Endogámicos C57BL , Neutrófilos/patología , ARN Mensajero/metabolismo , Factores de Tiempo , Distribución Tisular , Factor de Necrosis Tumoral alfa/genética

RESUMEN

A flow cytometric phenotype for isolated adult central nervous system (CNS) ramified microglia was previously defined (CD45low CD11b/c+) in the Lewis strain rat, that clearly distinguished these cells from all blood-derived leucocytes, the latter being CD45high. Consistent with the reported lack of major histocompatibility complex (MHC) expression in the CNS, isolated microglia were mostly MHC class II-. Employing these phenotypic criteria, we now show that a proportion of microglia in Brown Norway (BN) strain rats are constitutively MHC class II+. In spinal cord, up to 25% of microglia are distinctly positive and most have some level of expression. In situ staining of MHC class II+ microglial cells in BN rats indicates that positive cells are typical of ramified microglia on the grounds of both morphological appearance and anatomical location. In Lewis (LEW) rats, the few MHC class II-expressing cells isolated from the normal CNS are CD45high blood-derived cells and not resident microglia. After infection of both LEW and BN rats with a neurotropic murine hepatitis virus (MHV-JHM), MHC class II was rapidly upregulated on microglia in the BN but not in the LEW strain. In the latter, inflammatory cells were the predominant MHC class II-expressing population. Nevertheless, most microglia in the LEW strain could, after some delay, be induced to express MHC class II after transfer of an experimental autoimmune encephalomyelitis (EAE)-inducing encephalitogenic T cell line. Paradoxically, strains resistant to EAE (exemplified by the BN) contained more constitutive MHC class II-expressing microglia than susceptible ones, when a variety of strains were examined. The results clearly establish that the normal CNS may contain MHC class II-expressing cells that are a resident rather than a transient blood-derived population. It is significant that this expression is strain related, but there is no evidence that microglial cell constitutive MHC class II expression predisposes to EAE susceptibility.

Asunto(s)

Sistema Nervioso Central/citología , Antígenos de Histocompatibilidad Clase II/inmunología , Macrófagos/inmunología , Neuroglía/inmunología , Animales , Sistema Nervioso Central/inmunología , Quimera , Encefalomielitis/inmunología , Femenino , Inmunidad Innata , Ratones , Fenotipo , Ratas , Ratas Endogámicas Lew , Regulación hacia Arriba

Asunto(s)

Procedimientos Quirúrgicos Cardíacos , Fatiga/enfermería , Complicaciones Posoperatorias/enfermería , Anciano , Procedimientos Quirúrgicos Cardíacos/rehabilitación , Humanos , Locomoción , Masculino , Evaluación en Enfermería

RESUMEN

We studied the feasibility of obtaining accurate coagulation studies from indwelling, heparinized radial artery catheters in 28 patients after cardiac surgery. PTT assay was chosen because of its frequent clinical use. Thrombin time assay was chosen because it is a component of the coagulation screening panel and because it is useful in assessing heparin contamination of specimens. We conclude that PTT results are reliable when the dwell volume (0.6 ml for the catheter and extension tubing in our study) and an additional 4.5 ml have been discarded (5.1 ml total discard volume). We recommend the collection of samples for thrombin time assay from a separate venous site because results on samples from heparinized arterial catheters are unpredictable.

Asunto(s)

Pruebas de Coagulación Sanguínea/normas , Catéteres de Permanencia , Heparina , Adulto , Arteria Braquial , Procedimientos Quirúrgicos Cardíacos , Cuidados Críticos , Humanos , Tiempo de Tromboplastina Parcial , Manejo de Especímenes/métodos , Tiempo de Trombina