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PURPOSE: The purpose of this study was to evaluate the anterior tibial translation (ATT) in the prone position after total knee arthroplasty (TKA). METHODS: Fifty subjects (50 knees) undergoing bi-cruciate substituting (BCS)-TKA (Journey II: Smith and Nephew) and age-gender matching 50 subjects (50 knees) undergoing posterior stabilizing (PS)-TKA, were included in this study. Approximately, six months after surgery, and when the subjects had recovered their range of knee motion, following the Mae's method, accurate lateral radiographic imaging of the knee was performed with full knee extension in both supine and prone positions. The maximal protrusion length of the femoral posterior component, posterior to the extension line parallel to the tibial shaft from the edge of the posterior tibial plateau, was measured on lateral radiographs. The difference in length between the prone and supine positions was regarded as the prone-ATT. The posterior protrusion length of the femoral component, and the prone-ATT were compared between BCS and PS-TKA. RESULTS: The posterior protrusion length of the femoral component in the supine position was BCS-TKA 4.3 ± 1.9 mm, and PS-TKA 8.7 ± 2.3 mm. The length in the prone position was BCS-TKA 4.8 ± 2.3 mm, and PS-TKA 10.7 ± 2.2 m. Posterior protrusion length of the femoral component was significantly larger in both positions in PS-TKA when compared with BCS-TKA. In PS-TKA, posterior protrusion length of the femoral condyle was significantly larger in the prone position when compared to the supine position. No significant difference was observed in BCS-TKA. Prone-ATT was significantly larger in PS-TKA (2 ± 1.9 mm) when compared to BCS-TKA (0.7 ± 2 mm). CONCLUSION: Even in a position corresponding to daily movement such as the prone position, ATT was significantly larger in PS-TKA, when compared to BCS-TKA.

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Purpose: The purpose of this study was to evaluate the reproducibility of the native anterior cruciate ligament (ACL) mid-substance cross sectional area in anatomic single-bundle ACL reconstruction. Methods: Fifty-eight subjects who were performed anatomic single-bundle ACL reconstruction were included. Cross section size of the ACL graft was calculated from the graft diameter during surgery. Computed tomography (CT) of the knee was performed pre-operatively. Following Iriuchishima's report, native ACL size was estimated from the axial CT image of intercondylar notch area of femur at the most distal level of Blumensaat's line (In the report, native ACL size was equal to 14% of the intercondylar notch area of femur). The measured ACL graft cross-sectional size and estimated native ACL size were compared and correlation was evaluated. Results: Measured ACL graft cross-sectional size was 49 ± 14 mm2. Measured intercondylar notch area of femur at the most distal level of Blumensaat's line was 372 ± 91.6 mm2, and estimated native ACL size was 53 ± 12.5 mm2. Measured ACL graft cross-section and estimated native ACL showed no significant size difference. Measured ACL graft cross-section and estimated native ACL had no significant size correlation. Conclusion: Native ACL cross-sectional size was reproduced in anatomic single-bundle ACL reconstruction. However, as measured ACL graft and estimated native ACL showed no size correlation, it is possible that size of native ACL might not be reproduced. Such cases would be susceptible to the risk of graft impingement or knee instability.

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The purpose of this study was to reveal the correlation and proportion between the anterior cruciate ligament (ACL) cross-sectional area and the femoral intercondylar notch area. Sixty-three subjects (33 female and 30 male) less than 50 years old were included in this study. All subjects complained of knee pain, although magnetic resonance imaging (MRI) showed no structural damage of the knee. Using the T2 axial slice of the MRI perpendicular to the bone shaft, the ACL cross-sectional area and the femoral intercondylar notch area were measured. Measurements were made at the most proximal (S1), ⅓ (S2), ⅔ (S3), and the most distal (S4) Blumensaat's line levels. The correlation and the proportion between the ACL cross-sectional area and the notch area were calculated. The ACL cross-sectional area was: S1: 35.9 ± 10mm2, S2: 59.9 ± 14mm2, S3: 67.2 ± 19.5mm2, and S4: 70.7 ± 20.3mm2. The notch area was: S1: 215.5 ± 43mm2, S2: 311.8 ± 65mm2, S3: 453.8 ± 86mm2, and S4: 503.7 ± 99.8mm2. The ACL cross-sectional area and the notch area were found to be significantly correlated at the S3 (Pearson's coefficient correlation: 0.510, p = 0.000) and S4 (Pearson's coefficient correlation: 0.529, p = 0.000) levels. The proportion of the ACL cross-sectional area to the notch area was 15% in S3 and 14% in S4. The ACL cross-sectional area was found to be significantly correlated with the femoral intercondylar notch area at the distal level of the Blumensaat's line. The ACL cross-sectional area was found to be approximately 15% of the notch area. The ACL cross-sectional area can be predicted by measuring the femoral intercondylar notch area. This finding can be useful for achieving greater accuracy in anatomical ACL reconstruction. LEVEL OF EVIDENCE: III.

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Purpose: The purpose of this study was to measure anterior cruciate ligament (ACL) volume in a newly reported multi-truncated pyramid shape simulation using axial magnetic resonance imaging (MRI) for the detailed knowledge of the ACL anatomy. Methods: Fifty subjects (27 female and 23 male, average age: 23 ± 7.8) visiting our clinic with knee pain and in whom MRI showed no structural injury were included in this study. Using the axial image of the MRI, four deferent levels of the cross-sectional area of the ACL were measured. ACL height was measured as the distance between the most proximal and distal slices of the MRI. ACL volume was calculated using a multi-truncated pyramid shape simulation. Femoral intercondylar notch height, area, and trans-epicondylar length (TEL) were also measured using MRI. Results: The measured top, proximal 1/3, distal 1/3, and bottom of the ACL cross-sectional area were, 36.8 ± 10.7, 59.9 ± 15.4, 66.4 ± 20.8, and 107.3 ± 21.1mm2, respectively. ACL height was 26.3 ± 3.9 mm. Using these data, the calculated ACL volume was 1755 ± 874mm3. Significant correlations were observed between ACL volume and notch height, area, and TEL. Conclusion: Similar ACL volume with previous reports was obtained in this simple and easy multi-truncated pyramid shape simulation from axial MRI evaluation. Significant correlation was observed between ACL volume and knee bony morphology. The ability of surgeons to measure ACL volume simply and effectively can be useful for the detailed ACL anatomical knowledge, and also for prediction and prevention of ACL injury.Level of evidence: IV, Case series.

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Purpose: The purpose of this systematic review was to review the anatomical reports concerning the anterior cruciate ligament (ACL) focusing on the mid-substance insertion and fan-like extension fibers, or direct and indirect insertions. Methods: Following the PRISMA, data collection was performed. PubMed, Web of Science, and the Cochran library were searched with the terms "anterior cruciate ligament reconstruction", "anatomy", and "cadaver". Studies were included when anatomical dissection of the ACL with cadavers was performed. Biomechanical studies without a detailed description of the anatomical dissection, reviews, and studies not including pictures of the anatomical specimens were excluded from this study. In the full article review, documentation of the mid-substance insertion and fan-like extension fibers, or direct and indirect insertions in the ACL morphology was evaluated in detail. Results: Fifty-seven studies were included for detailed evaluation. In 2006, Mochizuki et al. reported a macroscopic differentiation between the mid-substance insertion and fan-like extension fibers in the ACL footprint. In 2010, Iwahashi et al. detected the existence of direct and indirect insertions within the femoral ACL footprint, microscopically. Following Mochizuki's report, anatomical evaluation of the mid-substance insertion and fan-like extension fibers, or direct and indirect insertions was reported in 16 of 51 ACL anatomical studies. In studies focusing on the morphology of the ACL, 16 of 28 studies addressed this subject. In these studies, the mid-substance insertion and fan-like extension fibers were differentiated macroscopically, and the direct and indirect insertions were differentiated microscopically within the ACL footprint. Fan-like extension fibers or indirect insertion was reported to surround the mid-substance insertion or direct insertion within the femoral ACL footprint. Conclusions: The results of this systematic review showed that, the existence of the mid-substance insertion and fan-like extension fibers, or direct and indirect insertions in ACL morphology is being recognized more widely. These structures should be taken into consideration when surgeons perform ACL surgery. Level of Evidence: III. Systematic review of Level-III studies. Supplementary Information: The online version contains supplementary material available at 10.1007/s43465-022-00695-4.

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PURPOSE: The purpose of this study was to investigate the correlation between femoral intercondylar notch volume and the characteristics of femoral tunnels in anatomical single bundle anterior cruciate ligament (ACL) reconstruction. METHODS: Fifty-one subjects (24 male and 27 female: median age 27: range 15-49), were included in this study. Anatomical single bundle ACL reconstruction was performed in all subjects using a trans-portal technique. Femoral tunnel length was measured intra-operatively. Three-dimensional computed tomography (3D-CT) was taken at pre and post-surgery. The intercondylar notch volume was calculated with a truncated-pyramid shape simulation using the pre-operative 3D-CT image. In the post-operative 3D-CT, the modified quadrant method was used to measure femoral ACL tunnel placement. RESULTS: Femoral tunnel placement was 47.6 ± 10.5% in the high-low (proximal-distal) direction, and 22.6 ± 5.4% in the shallow-deep (anterior-posterior) direction. Femoral tunnel length was 35.3 ± 4.4 cm. Femoral intercondylar notch volume was 8.6 ± 2.1cm3. A significant correlation was found between femoral intercondylar notch volume and high-low (proximal-distal) femoral tunnel placement (Pearson's coefficient correlation: 0.469, p = 0.003). CONCLUSION: Femoral ACL tunnel placement at a significantly lower level was found in knees with large femoral intercondylar notch volume in the trans-portal technique. For the clinical relevance, although the sample size of this study was limited, surgeons can create femoral ACL tunnel low (distal) in the notch where close to the anatomical ACL footprint in the knees with large femoral intercondylar notch volume. LEVEL OF EVIDENCE: III.

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The purpose of this study was to assess the influence of tibial spine location on tibial tunnel placement in anatomical single-bundle anterior cruciate ligament (ACL) reconstruction using three-dimensional computed tomography (3D-CT). A total of 39 patients undergoing anatomical single-bundle ACL reconstruction were included in this study (30 females and 9 males; average age: 29 ± 15.2 years). In anatomical single-bundle ACL reconstruction, the tibial and femoral tunnels were created close to the anteromedial bundle insertion site using a transportal technique. Using postoperative 3D-CT, accurate axial views of the tibia plateau were evaluated. By assuming the medial and anterior borders of the tibia plateau as 0% and the lateral and posterior borders as 100%, the location of the medial and lateral tibial spine, and the center of the tibial tunnel were calculated. Statistical analysis was performed to assess the correlation between tibial spine location and tibial tunnel placement. The medial tibial spine was located at 54.7 ± 4.5% from the anterior border and 41.3 ± 3% from the medial border. The lateral tibial spine was located at 58.7 ± 5.1% from the anterior border and 55.3 ± 2.8% from the medial border. The ACL tibial tunnel was located at 34.8 ± 7.7% from the anterior border and 48.2 ± 3.4% from the medial border. Mediolateral tunnel placement was significantly correlated with medial and lateral tibial spine location. However, for anteroposterior tunnel placement, no significant correlation was found. A significant correlation was observed between mediolateral ACL tibial tunnel placement and medial and lateral tibial spine location. For clinical relevance, tibial ACL tunnel placement might be unintentionally influenced by tibial spine location. Confirmation of the ACL footprint is required to create accurate anatomical tunnels during surgery. This is a Level III; case-control study.

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PURPOSE: The purpose of this study was to reveal the possible influence of the tibial spine area on the occurrence of ACL injury. METHODS: Thirty-nine subjects undergoing anatomical ACL reconstruction (30 female, 9 male: average age 29 ± 15.2) and 37 subjects with intact ACL (21 female, 16 male: average age 29 ± 12.5) were included in this study. In the anterior-posterior (A-P) and lateral knee radiograph, the tibial spine area was measured using a PACS system. In axial knee MRI exhibiting the longest femoral epicondylar length, the intercondylar notch area was measured. Tibial spine area, tibial spine area/body height, and tibial spine area/notch area were compared between the ACL tear and intact groups. RESULTS: The A-P tibial spine area of the ACL tear and intact groups was 178 ± 34 and 220.7 ± 58mm2, respectively. The lateral tibial spine area of the ACL tear and intact groups was 145.7 ± 36.9 and 178.9 ± 41.7mm2, respectively. The tibial spine area was significantly larger in the ACL intact group when compared with the ACL tear group (A-P: p = 0.02, lateral: p = 0.03). This trend was unchanged even when the tibial spine area was normalized by body height (A-P: p = 0.01, lateral: p = 0.02). The tibial spine area/notch area of the ACL tear and intact groups showed no significant difference. CONCLUSION: The A-P and lateral tibial spine area was significantly smaller in the ACL tear group when compared with the ACL intact group. Although the sample size was limited, a small tibial spine might be a cause of knee instability, which may result in ACL injury. LEVEL OF EVIDENCE: Level III.

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PURPOSE: Smaller femoral intercondylar notch volume has been identified as a risk factor for anterior cruciate ligament injury. The present study aims to investigate differences in the intercondylar notch volume based on differences in the morphology of Blumensaat's line. METHODS: Eighty-eight (88) subjects (42 male and 46 female: median age 27: range 15-49), were included in this study. Using 3-dimensional computed tomography (3D-CT), the volume of the intercondylar notch was calculated using a truncated-pyramid shape simulation with the formula: [Formula: see text]. Femoral condyle height (h) was measured in the sagittal plane of the knee in 3D-CT. The area of the intercondylar notch was measured in the axial slice containing the most proximal level (S1) and most distal level (S2) of Blumensaat's line. In the sagittal view of the knee, Blumensaat's line morphology was classified into either straight or hill type. Statistical analysis was performed to compare h, S1, S2, and notch volume between the straight and hill type groups. RESULTS: Thirty-six subjects were classified as having straight type morphology and 52 subjects were classified as having hill type morphology. The measured h, S1, and S2, of the straight and hill types were 29 ± 4 and 31 ± 4 mm, 213 ± 72 and 205 ± 51 mm2, 375 ± 114 and 430 ± 94 mm2, respectively. The calculated femoral intercondylar notch volume of the straight and hill types was 8.1 ± 2 and 9.5 ± 2 cm3, respectively. Straight type knees showed significantly smaller S2 (p = 0.04), and notch volume (p = 0.01) when compared with hill type knees. CONCLUSION: Intercondylar notch volume was significantly smaller in knees with straight type Blumensaat's line morphology. Considering that Blumensaat's line represents the roof of the femoral notch, morphological variations in Blumensaat's line are likely to reflect variation in notch volume. For clinical relevance, as a smaller notch volume is a risk factor for ACL injury, straight type Blumensaat's line may also be considered a potential risk factor for ACL injury. LEVEL OF EVIDENCE: Level III.

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The purpose of this systematic review was to reveal the trend in surgical technique and tunnel targets points and placement in anatomical single-bundle anterior cruciate ligament (ACL) reconstruction. Following the Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA) statement, data collection was performed. PubMed, EMBASE, and Cochran Review were searched using the terms "anterior cruciate ligament reconstruction," "anatomic or anatomical," and "single bundle." Studies were included when they reported clinical results, surgical technique, and/or tunnel placement evaluation. Laboratory studies, technical reports, case reports, and reviews were excluded from this study. From these full article reviews, graft selection, method of creating the femoral tunnel, and femoral and tibial tunnel target points and placement were evaluated. In the 79 studies included for data evaluation, the selected grafts were: bone patella tendon bone autograft (12%), and hamstring autograft (83%). The reported methods of creating the femoral tunnel were: transportal technique (54%), outside-in technique (15%), and transtibial technique (19%). In the 60 studies reporting tunnel target points, the target point was the center of the femoral footprint (60%), and the center of the anteromedial bundle footprint (22%). In the 23 studies evaluating tunnel placement, the femoral tunnel was placed in a shallow-deep direction (32.3%) and in a high-low direction (30.2%), and the tibial tunnel was placed from the anterior margin of the tibia (38.1%). The results of this systematic review revealed a trend in anatomical single-bundle ACL reconstruction favoring a hamstring tendon with a transportal technique, and a tunnel target point mainly at the center of the ACL footprint. The level of evidence stated is Systematic review of level-III studies.

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PURPOSE: The purpose of this study was to measure the femoral intercondylar notch volume using a truncated-pyramid shape simulation and compare this volume between anterior cruciate ligament (ACL) injured and intact subjects. METHODS: Forty-seven subjects diagnosed with ACL tear by MRI (22 male and 25 female: median age 26: range 15-49), and 41 subjects in which knee MRI was performed and no ACL injury detected (20 males and 21 females: median age 27: range 16-49), were included in this study. Using three-dimensional computed tomography (3D-CT), the axial femoral intercondylar notch area was measured in the slice containing the most proximal (S1) and most distal (S2) level of Blumensaat's line. Femoral condyle height (h) was measured using a sagittal view of knees in 3D-CT. The truncated-pyramid shape simulation was calculated as: Volume = [Formula: see text]. Statistical analysis was performed to compare S1, S2, notch height, and notch volume between the ACL-injured and intact groups. RESULTS: The measured S1, S2, and the notch height of the ACL-injured and intact groups were 201 ± 64 and 214 ± 50mm2, 370 ± 91 and 461 ± 94mm2, and 31 ± 3 and 30 ± 4mm, respectively. The calculated femoral intercondylar notch volume of the ACL-injured and intact groups was 8.6 ± 2.2 and 9.9 ± 2.6cm3, respectively. The ACL intact group showed significantly larger S2 and notch volume when compared with the ACL-injured group. CONCLUSION: For clinical relevance, notch volume and most distal axial notch area parameters were significantly larger in ACL intact subjects. The truncated-pyramid shape simulation is an easy and cost-effective method to evaluate intercondylar notch volume. In knees with small femoral intercondylar notch volume, attention is needed to prevent ACL injury. LEVEL OF EVIDENCE: Level III.

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PURPOSE: The purpose of this study was to reveal the influence of the variance in width between the tibial spine and the femoral intercondylar notch on the occurrence of ACL injury. METHODS: Thirty-nine subjects undergoing anatomical ACL reconstruction (30 female, 9 male; average age 29 ± 15.2) and 37 subjects with intact ACL (21 female, 16 male; average age 29 ± 12.5) were included in this study. In the anterior-posterior knee radiograph, tibial spine height, and the length between the top of the medial and lateral tibial spine (tibial spine width) were measured. In axial knee MRI exhibiting the longest femoral epicondylar length, intercondylar notch outlet length was measured and notch width index was calculated. Tibial spine width/notch outlet length, and tibial spine width/notch width index were compared between the ACL tear and intact groups. RESULTS: Tibial spine width/notch outlet length of the ACL tear and intact groups was 0.6 ± 0.1 and 0.7 ± 0.1, respectively. Tibial spine width/notch width index of the ACL tear and intact groups was 0.4 ± 0.1, and 0.6 ± 0.1, respectively. Both parameters were significantly larger in the ACL intact group. CONCLUSION: Both tibial spine width/notch outlet length and tibial spine width/notch width index were significantly smaller in the ACL tear group when compared with the ACL intact group. The occurrence of ACL injury influenced by the variance in width between the tibial spine and the femoral intercondylar notch. LEVEL OF EVIDENCE: III.

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PURPOSE: The purpose of this study was to reveal the influence of the morphological variations of the Blumensaat's line on femoral tunnel position in anatomical anterior cruciate ligament (ACL) reconstruction. METHODS: Thirty-eight subjects undergoing anatomical single-bundle ACL reconstruction were included in this study (22 female, 16 male: median age 45: 15-63). Using a trans-portal technique, the femoral tunnel was targeted to reproduce the center of antero-medial bundle. Following Iriuchishima's classification, the morphology of the Blumensaat's line was classified into straight and hill types (small and large hill types). Femoral ACL tunnel position was evaluated using the quadrant method. When the quadrant method grid was applied, the baseline of the grid was matched to the anterior part of the Blumensaat's line, without considering the existence of a hill. Using pre-operative 3D-CT data, the axial and sagittal morphology of the knee was also compared, establlishing straight and hill types. RESULTS: There were 12 straight type knees and 26 hill type knees (7 small hill type knees and 19 large hill type knees). The femoral tunnel position in straight type knees was 23.6 ± 3.7% in the shallow-deep direction, and 41.3 ± 8.2% in the high-low direction. In hill type knees, the tunnel position was 27 ± 4.7% in the shallow-deep direction, and 51 ± 10.1% in the high-low direction. The femoral tunnel was placed significantly more shallow and lower in hill type knees when compared with straight type knees. CONCLUSION: Femoral ACL tunnel placement was significantly influenced by the morphological variations of the Blumensaat's line. As detecting morphological variation in arthroscopic surgery is difficult, surgeons should confirm such variations pre-operatively using radiograph or CT so as to avoid making extremely shallow and low tunnels in hill type knees. LEVEL OF EVIDENCE: Case-controlled study, III.

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PURPOSE: The purpose of this study was to reveal the correlation between femoral tunnel length in anatomical single-bundle anterior cruciate ligament (ACL) reconstruction and body size and/or knee morphology. METHODS: Thirty-one subjects undergoing anatomical single-bundle ACL reconstruction were included in this study (20 female, 11 male; median age 46, 15-63). Pre-operative height, body weight, and body mass index (BMI) were measured. In pre-operative magnetic resonance imaging, the thickness of the quadriceps tendon and the whole anterior-posterior (AP) length of the knee were measured using the sagittal slice. Using post-operative three-dimensional computed tomography, accurate axial and lateral views of the femoral condyle were evaluated. The correlation of femoral tunnel length, which was measured intra-operatively, with the height, weight, BMI, quadriceps tendon thickness, AP length of the knee, trans-epicondylar length, the notch area (axial), length of Blumensaat's line, and the height and area of the lateral wall of the femoral intercondylar notch were statistically analyzed. Tunnel placement was also evaluated using a Quadrant method. RESULTS: The average femoral tunnel length was 35.6 ± 4.4 mm. The average height, body weight, and BMI were 162.7 ± 7.2 cm, 61.9 ± 10 kg, and 23.4 ± 3.5, respectively. Femoral tunnel length was significantly correlated with height, body weight and the height and area of lateral wall of the femoral intercondylar notch, and the length of the Blumensaat's line. CONCLUSION: For clinical relevance, the risk of creating a femoral tunnel of insufficient length in anatomical single-bundle ACL reconstruction exists in subjects with small body size. Surgeons should pay careful attention to prevent this from occurring. LEVEL OF EVIDENCE: Case-controlled study, Level III.

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PURPOSE: To evaluate the effect of knee flexion angle for hamstring graft fixation, full extension (FE), or 30°, on acceleration of the knee motion during pivot-shift testing after either anatomic or nonanatomic anterior cruciate ligament (ACL) reconstruction using triaxial accelerometry. METHODS: Two types of ACL reconstructions (anatomic and nonanatomic) using 2 different angles of knee flexion during graft fixation (FE and 30°) were performed on 12 fresh-frozen human knees making 4 groups: anatomic-FE, anatomic-30°, nonanatomic-FE, and nonanatomic-30°. Manual pivot-shift testing was performed at ACL-intact, ACL-deficient, and ACL-reconstructed conditions. Three-dimensional acceleration of knee motion was recorded using a triaxial accelerometer. RESULTS: The anatomic-30° group showed the smallest overall magnitude of acceleration among the ACL-reconstructed groups (P = .0039). There were no significant differences among the anatomic-FE group, the nonanatomic-FE group, and the nonantomic-30° group (anatomic-FE vs nonanatomic-FE, P = .1093; anatomic-FE vs nonanatomic-30°, P = .8728; and nonanatomic-FE vs nonanatomic-30°, P = .1093). After ACL transection, acceleration was reduced by ACL reconstruction with the exception of the nonanatomic-FE group that did not show a significant difference when compared with the ACL-deficient (P = .4537). CONCLUSIONS: The anatomic ACL reconstruction with the graft fixed at 30° of knee flexion better restored rotational knee stability compared with FE. An ACL graft fixed with the knee at FE in anatomic position did not show a significant difference compared with the nonanatomic ACL reconstructions. CLINICAL RELEVANCE: Knee flexion angle at the time of graft fixation for ACL reconstruction can be considered to maximize the rotational knee stability.

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OBJECTIVE: The purpose of this study was to investigate the effectiveness of photodynamic therapy (PDT) with Na-pheophorbide A in anticancer treatment, using osteosarcoma cells in vitro. BACKGROUND DATA: It has been reported that PDT with chlorophyll derivatives inhibits the proliferation of various cancer cells. However, there have been no reports that have evaluated the effectiveness of PDT in suppressing osteosarcoma cells. MATERIALS AND METHODS: Uptake of Na-pheophorbide A into Hu09 cells (osteosarcoma cells) was assayed using fluorescence microscopy following incubation of the cells with 28 µmol/L of Na-pheophorbide A. The viability of Hu09 cells after PDT treatment was assessed using trypan blue dye staining and MTS assays. PDT-induced apoptosis was determined by evaluation of the activity of selected members of the caspase family and by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining of cells. RESULTS: Na-pheophorbide A uptake by cells was rapid, being observed after 60 min of treatment, and Na-pheophorbide A persisted in cells for >24 h. PDT treatment decreased cell viability compared with the control group, indicating high cytocidal activity of PDT. This cytocidal effect was dependent upon drug concentration, light dose, and the number of irradiation times. An increase in the number of cells positive for TUNEL staining and increases in the activity of caspases-3, -8 and -9 were observed in the first 2 h after PDT treatment. CONCLUSIONS: A cytotoxic effect of PDT with Na-pheophorbide A on an osteosarcoma cell line in vitro was shown. Caspase activity assays suggested that PDT with Na-pheophorbide A induced an apoptotic change in HuO9 cells, mainly via activation of mitochondrial caspase -9 and -3 pathways.

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PURPOSE: Recently, single-bundle (SB) anterior cruciate ligament (ACL) reconstruction has been advanced by the anatomic concept, but the biomechanical outcome of the anatomic method has not been fully investigated, especially for rotational instability. Anatomic SB and the single over-the-top procedures are the treatment of choice for primary cases and revision or skeletally immature cases, respectively. The purpose of this study was to investigate the dynamic rotational instability of anatomic SB and over-the-top reconstruction during a pivot shift test using triaxial accelerometry. METHODS: Eight fresh frozen human cadaveric knees were used in this study. Rotational instability measurement was conducted during a pivot shift test by the use of a triaxial accelerometer attached to the tibia. The tests were performed in the ACL-intact, ACL-deficient and ACL-reconstructed knees with two different procedures (anatomic SB and over-the-top). The acceleration in three directions and the magnitude of acceleration were measured to evaluate rotational instability and compare between four different knee states. RESULTS: The overall magnitude of acceleration was significantly different (P < 0.01) between the ACL-intact knees and the ACL-deficient knees. Both anatomic SB and over-the-top ACL reconstruction significantly reduced the overall magnitude of acceleration compared to the ACL-deficient knees, but still had larger accelerations compared to the ACL-intact knees. There was no significant difference for the overall magnitude of acceleration between anatomic SB and over-the-top reconstruction procedure. CONCLUSION: Over-the-top reconstruction provides comparable result to anatomic SB reconstruction in terms of controlling the dynamic rotational stability. Over-the-top reconstruction might be one of the options for revision cases and in skeletally immature patients.

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PURPOSE: Conventional transtibial technique fails to restore the rotational knee stability in spite of successful anterior laxity, while anatomic anterior cruciate ligament reconstruction using the anteromedial portal technique has been developed expecting better rotational kinematics because of closer reproduction of the native anterior cruciate ligament anatomy. However, the rotational instability after those two procedures has not been fully examined especially in terms of dynamic component of the rotational stability. The purpose was to assess the effect of anatomic versus non-anatomic tunnel placement on rotational knee stability after anterior cruciate ligament reconstruction using triaxial accelerometry. METHODS: Sixteen porcine knees underwent a manual pivot-shift test at four different conditions: (1) anterior cruciate ligament intact, (2) anterior cruciate ligament deficient, (3) non-anatomic transtibial reconstruction, and (4) anatomic anteromedial portal reconstruction. The three-dimensional acceleration of knee motion during the pivot-shift test was recorded using a triaxial accelerometer. RESULTS: Both anterior cruciate ligament reconstructions decreased significantly the acceleration of the pivot-shift test from the increased level in the anterior cruciate ligament-deficient condition. However, the transtibial technique fails to reach the intact level of acceleration, while the anteromedial portal technique reduced the acceleration to even less than the intact level. CONCLUSION: The transtibial anterior cruciate ligament reconstruction could not restore the dynamic rotational stability of the intact knee, whereas the anteromedial portal technique restored the dynamic rotational stability closer to the intact level. LEVEL OF EVIDENCE: III.

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PURPOSE: To measure the acceleration in multiple directions of the rotational instability in ACL deficient models using porcine knees. METHODS: Ten porcine knees were tested with ACL intact and tear models. The pivot shift test was performed manually, and the acceleration of the pivot shift phenomenon was recorded by the use of a triaxial accelerometer. Tests were repeated in four different ACL states: (1) intact; (2) partial AM deficient; (3) complete AM deficient, and (4) complete ACL (AM and PL) deficient. The acceleration in three directions and the magnitude of acceleration were measured to evaluate rotational instability and compare between different ACL conditions. RESULTS: Significantly increased accelerations were observed in the complete deficient ACL model, while the partial ACL tear models demonstrated a slight increase without statistical significance. The accelerometer detected stepwise increases in the acceleration with the extent of ACL tear. Additionally, the PL bundle exhibited the largest contribution for rotational instability (80.4%) when compared with the AM (19.5%) bundles. CONCLUSION: Triaxial accelerometer could serve as a quantitative evaluation of rotational instability. The present study demonstrated that PL bundle has the most important contribution for rotational instability (80.4%) when compared to IM bundle (0.01%) and AM bundle (19.5%) in porcine knee model.

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OBJECTIVE: In this study, we examined the therapeutic effect of photodynamic therapy (PDT) using the photosensitizer Na-Pheophorbide a (Na-Phde a) on osteomyelitis models in rats. BACKGROUND: Osteomyelitis is one of the most serious infectious problems in the orthopedic field. Recently, as a new clinical approach against septic arthritis, an experimental in vivo and in vitro model for the inactivation of methicillin-resistant-Staphylococcus aureus by PDT using Na-Phde a has been developed. METHODS: Methicillin-sensitive Staphylococcus aureus (MSSA) was injected into the tibia of the rats to create osteomyelitis models (n = 10, 10 legs). A total of 560 µmol/l of Na-Phde a solution was injected into five of these tibial osteomyelitis models (five legs) 48 h after the initial MSSA infection. Sixty minutes after the Na-Phde a injection, a semiconductor laser (125 mW, 670 nm) was used to irradiate the models for 10 min with a total energy of 93.8 J/mm(2). As a control group, five rats (five legs) were treated with a phosphate buffered saline injection at 48 h after MSSA infection. Weight and leg perimeter changes were plotted. Bacterial growth, histological examination and radiological examination were evaluated at 14 days after initial treatment. RESULTS: PDT with Na-Phde a significantly prevented leg swelling. In the PDT group, bone destruction owing to osteomyelitis was inhibited not only histologically but also radiographically. CONCLUSIONS: The results in these experiments show that PDT using Na-Phde a improved osteomyelitis in rats. This suggests that PDT using Na- Phde a can be a useful treatment for osteomyelitis.

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