RESUMEN
The safety of experimental chemical cholera monovalent vaccine in tablets, produced by the institute "Microbe" (Saratov, USSR), has been studied. The study has shown that the vaccine, administered to adult rabbits and germ-free suckling rabbits by the enteral route, retains residual toxicity, mainly due to the presence of O-antigen. One or two administrations of 1-2 human doses of this preparation to adult rabbits induce minimal structural changes admissible from the viewpoint of safety. After immunization made in two administrations immunobiological transformation develops more rapidly and is more pronounced than after immunization in a single administration.
Asunto(s)
Vacunas contra el Cólera/toxicidad , Administración Oral , Animales , Animales Lactantes , Antígenos Bacterianos/administración & dosificación , Antígenos Bacterianos/toxicidad , Vacunas contra el Cólera/administración & dosificación , Relación Dosis-Respuesta Inmunológica , Vida Libre de Gérmenes , Intestinos/efectos de los fármacos , Intestinos/patología , Antígenos O , Conejos , Comprimidos , Factores de Tiempo , Vacunas Sintéticas/administración & dosificación , Vacunas Sintéticas/toxicidad , Vibrio cholerae/inmunologíaRESUMEN
Immunization with live plague vaccine has been shown to give no protection to thymectomized mice from subcutaneous challenge with Y. pestis virulent strain. Under the action of the vaccine or individual Y. pestis antigens (fraction I) the functional and morphological activation of thymocytes and macrophages is observed, more pronounced in C57BL/6 mice and less pronounced in CBA mice. Y. pestis antigenic preparations (fractions I and II, pesticin) act as T-cell mitogens and are thus capable of inducing the in vitro proliferation of thymocytes. At the same time the in vivo action of fraction II induces a decrease in the level of lymphocytes in the peripheral blood of mice and the destruction of lymphocytes in their thymus and spleen.
Asunto(s)
Antígenos Bacterianos/inmunología , Peste/inmunología , Yersinia pestis/inmunología , Animales , Antígenos Bacterianos/toxicidad , Vacunas Bacterianas/inmunología , Vacunas Bacterianas/toxicidad , Haplotipos/inmunología , Inmunidad Celular/inmunología , Inmunización , Ratones , Ratones Endogámicos , Fagocitos/inmunología , Linfocitos T/inmunología , Timo/inmunologíaRESUMEN
Germ-free suckling rabbits and minipigs can be recommended as models suitable for the study of different aspects of the pathogenesis of cholera intoxication. In minipigs, individual representatives of intestinal autochthonous microflora produce different effect on the sensitivity of the animals to the toxigenic and choleragenic action of Vibrio cholerae antigen introduced by oral administration, that should also be taken into consideration in the determination of residual toxicity during the trial of new vaccine preparations against cholera.
Asunto(s)
Cólera/etiología , Vida Libre de Gérmenes , Animales , Animales Lactantes , Antígenos Bacterianos/administración & dosificación , Cólera/microbiología , Cólera/patología , Toxina del Cólera/administración & dosificación , Modelos Animales de Enfermedad , Relación Dosis-Respuesta a Droga , Intestinos/microbiología , Antígenos O , Conejos , Porcinos , Porcinos Enanos , Vibrio cholerae/inmunologíaRESUMEN
The data obtained during the study of the morphological changes in the internal organs and blood of 6 mouse strains at the early stages of anti-plague immunity are presented. It has been established that the introduction of 5.10(3) and 1.10(5) m. b. of the EV vaccine strain resulted in the development of morphological changes typical of residual virulence. The degree of these changes depended on the haplotype of the animals and the dose used. The most resistant to injury induced by EV strain were CBA mice (haplotype H-2k) and the most sensitive were C57BL/6 mice (haplotype H-2b).
Asunto(s)
Vacuna contra la Peste/efectos adversos , Yersinia pestis/inmunología , Glándulas Suprarrenales/patología , Animales , Haplotipos , Hígado/patología , Ratones , Ratones Endogámicos C57BL , Ratones Endogámicos CBA , Ratones Endogámicos , Vacuna contra la Peste/administración & dosificación , Bazo/patología , Yersinia pestis/aislamiento & purificación , Yersinia pestis/patogenicidadRESUMEN
Germ-free minipigs, previously treated with bacteroids, develop cholinergic reaction after the intragastric administration of Vibrio cholerae exotoxin. The intensity of this reaction, disturbances in homeostasis, and the character of morphological changes depend on the dose of choleragen, the bacteroid strain, and the presence of the concomitant (Escherichia coli) and residual microflora in the intestine.
Asunto(s)
Cólera/etiología , Intestinos/microbiología , Animales , Bacteroides , Cólera/microbiología , Cólera/patología , Toxina del Cólera/administración & dosificación , Escherichia coli , Vida Libre de Gérmenes , Histocitoquímica , Intestinos/enzimología , Intestinos/patología , Porcinos , Porcinos EnanosRESUMEN
As a result of the enteral and combined subcutaneous-enteral immunization of adult rabbits with cholerogen toxoid and the specific fraction of chemical cholera vaccine in tablets immunological transformation occurred in the organism and a "protective" barrier consisting of antibody-containing cells was formed in the intestine on days 3-5. The combined method of immunization with cholera vaccine proved to be essentially as effective as enteral immunization in 2 administrations.
Asunto(s)
Vacunas contra el Cólera/administración & dosificación , Cólera/prevención & control , Vacunación , Fosfatasa Ácida/metabolismo , Adenosina Trifosfatasas/metabolismo , Administración Oral , Animales , Anticuerpos Antibacterianos/biosíntesis , Células Productoras de Anticuerpos , Apéndice/inmunología , Cólera/inmunología , Glucosafosfato Deshidrogenasa/metabolismo , Inyecciones Subcutáneas , Ganglios Linfáticos/inmunología , Ganglios Linfáticos Agregados/inmunología , Células Plasmáticas/inmunología , Conejos , Bazo/inmunología , ComprimidosRESUMEN
Three fractions isolated from the culture fluid of the 569B cholera vibrio strain were studied. The fractions differed by the extent of purification, and the content of the toxid and the O-antigen. In intraintestinal application to rabbits all of them caused formation of antitoxins and vibriocidal antibodies in the blood serum. The immunizing dose of the preparations in intraintestinal administration exceeded the dose required for subcutaneous application. Fraction I should be used for Producing tablet form of cholera vaccine because it was least toxic and provided the greatest yield.