Asunto(s)
ADN Bacteriano/genética , Mycobacterium tuberculosis/genética , Técnicas de Amplificación de Ácido Nucleico/métodos , Tuberculosis/diagnóstico , Humanos , Mycobacterium tuberculosis/aislamiento & purificación , Reproducibilidad de los Resultados , Sensibilidad y Especificidad , Tuberculosis/microbiologíaRESUMEN
The aim of this study was to examine the antinociceptive potential of leaves of Ixora coccinea (family: Rubeaceae). One of four doses (500, 750, 1000 or 1500 mg/kg, n = 8/dose) of aqueous leaf extract (ALE) or 1 ml of distilled water was orally administered to male rats and antinociceptive activity was ascertained using three models ofnociception (tail flick, hot plate and formalin tests). The results showed that ALE possesses considerable antinociceptive activity (when evaluated in hot plate and formalin test but not in tail flick test). The antinociceptive activity of the ALE had a rapid onset (within 1h) and a fairly long duration of action (up to 5 h) with a peak effect at 3 h. Further, the antinociceptive activity was dose-dependent and was not associated with harmful side-effects or toxicity even following subchronic administration. The antinociceptive action was mediated centrally at the supraspinal level mainly via dopaminergic mechanism. In addition, it is likely that antioxidant activity of the ALE could have played an auxiliary role in inducing antinociception. Dopaminergic and antioxidative activities of ALE could arise, respectively, from its quaternary base alkaloid and flavonoid constituents.
Asunto(s)
Analgésicos/farmacología , Extractos Vegetales , Hojas de la Planta/metabolismo , Alanina Transaminasa/sangre , Animales , Antioxidantes/metabolismo , Aspartato Aminotransferasas/sangre , Membrana Celular/metabolismo , Creatinina/sangre , Dopamina/metabolismo , Femenino , Fertilidad/efectos de los fármacos , Formaldehído/metabolismo , Calor , Masculino , Músculos/efectos de los fármacos , Músculos/metabolismo , Dolor , Prostaglandinas/biosíntesis , Prostaglandinas/metabolismo , Distribución Aleatoria , Ratas , Receptores Dopaminérgicos/metabolismo , Receptores Opioides/metabolismo , Factores de Tiempo , Urea/metabolismo , Agua/metabolismoRESUMEN
Barringtonia racemosa Linn. (Family Lecythidaceae) possess several bioactivities and is used in traditional medicine of Sri Lanka, but its analgesic potential has not been investigated so far. The aim of this study was therefore to examine the antinociceptive potential of an aqueous bark extract (500, 750, 1000 or 1500 mg/kg) of B. racemosa in male rats using three models of nociception (tail flick, hot plate and formalin tests). The results showed that the extract has antinociceptive activity (when evaluated in hot plate and formalin test but not in tail flick test) without producing unwanted side effects or toxicity. Further, the extract did not alter fertility, gestational length, peri- and neonatal development and appears to be non-teratogenic. The antinociceptive effect was mediated mainly via opioid mechanisms. Such inhibition of pain could arise from phenolic and steroidal constituents as was shown to be present in the extract.