RESUMEN

OBJECTIVES: To engineer broad spectrum resistance in potato using different expression strategies. RESULTS: The previously identified Ribosome-Inactivating Protein from Phytolacca heterotepala was expressed in potato under a constitutive or a wound-inducible promoter. Leaves and tubers of the plants constitutively expressing the transgene were resistant to Botrytis cinerea and Rhizoctonia solani, respectively. The wound-inducible promoter was useful in driving the expression upon wounding and fungal damage, and conferred resistance to B. cinerea. The observed differences between the expression strategies are discussed considering the benefits and features offered by the two systems. CONCLUSIONS: Evidence is provided of the possible impact of promoter sequences to engineer BSR in plants, highlighting that the selection of a suitable expression strategy has to balance specific needs and target species.

Asunto(s)

Resistencia a la Enfermedad , Expresión Génica , Organismos Modificados Genéticamente/inmunología , Enfermedades de las Plantas/prevención & control , Proteínas Recombinantes/metabolismo , Proteínas Inactivadoras de Ribosomas/metabolismo , Solanum tuberosum/inmunología , Botrytis/inmunología , Botrytis/patogenicidad , Regulación de la Expresión Génica de las Plantas , Organismos Modificados Genéticamente/genética , Phytolacca/enzimología , Phytolacca/genética , Enfermedades de las Plantas/microbiología , Proteínas Recombinantes/genética , Rhizoctonia/inmunología , Rhizoctonia/patogenicidad , Proteínas Inactivadoras de Ribosomas/genética , Solanum tuberosum/genética