RESUMEN

The reporter gene xylE (encoding catechol 2,3-dioxygenase) has been modified for a more rational use in Streptomyces. Two reporter fragments, one containing xylE, and the other containing also the upstream gene xylT (which encodes a soluble ferredoxin), have been constructed to allow precise fusion of regulatory regions to the reporter genes. Identical fusions of these xylE and xylTE reporter fragments to the Streptomyces dagA and tipA promoters, in low and high copy number plasmids, show that the levels of xylE mRNA and catechol 2,3-dioxygenase activities are significantly higher when xylT is present.

Asunto(s)

Proteínas Bacterianas , Dioxigenasas , Ferredoxinas/genética , Genes Reporteros , Oxigenasas/genética , Streptomyces griseus/genética , Secuencia de Bases , Catecol 2,3-Dioxigenasa , ADN Bacteriano/química , Ferredoxinas/aislamiento & purificación , Ferredoxinas/fisiología , Regulación Bacteriana de la Expresión Génica , Vectores Genéticos , Datos de Secuencia Molecular , Oxigenasas/aislamiento & purificación , Regiones Promotoras Genéticas , Mapeo Restrictivo , Streptomyces/genética , Streptomyces/metabolismo , Streptomyces griseus/metabolismo