RESUMEN

Neospora caninum is a major cause of reproductive loss in cattle worldwide as it leads to abortion and animal repositioning. Although Toxoplasma gondii does not cause a reproductive problem in cattle, consuming raw or uncooked beef poses the risk of transmission. This study aimed to evaluate the occurrence of anti-N. caninum and anti-T. gondii antibodies in dairy cattle in the West and Northwest regions of São Paulo State, Brazil. A total of 653 serum samples from dairy cows were analyzed using an indirect immunofluorescence assay (IFA). Epidemiological data from the farms were associated with the serological results of the animals by logistic regression based on the presence of antibodies. The frequencies of the antibodies against N. caninum and T. gondii were 41.6% (272/653) and 11.5% (75/653), respectively. A statistically significant association was observed between: the serum anti-N. caninum antibodies and breed, history of food supplementation for calves, introduction of outside animals that later presented reproductive problems, and history of reproductive problems by the trimester of gestation. The present study highlights the importance of neosporosis in dairy cattle in the study regions and that the inclusion of this parasite in the investigation of animals with reproductive disorders is important.

Asunto(s)

Anticuerpos Antiprotozoarios , Enfermedades de los Bovinos , Coccidiosis , Neospora , Toxoplasma , Toxoplasmosis Animal , Animales , Bovinos , Neospora/inmunología , Brasil/epidemiología , Coccidiosis/veterinaria , Coccidiosis/epidemiología , Enfermedades de los Bovinos/epidemiología , Enfermedades de los Bovinos/parasitología , Toxoplasmosis Animal/epidemiología , Toxoplasmosis Animal/diagnóstico , Factores de Riesgo , Estudios Seroepidemiológicos , Toxoplasma/inmunología , Femenino , Anticuerpos Antiprotozoarios/sangre , Industria Lechera , Técnica del Anticuerpo Fluorescente Indirecta/veterinaria

RESUMEN

Objective: To analyze data of patients with symptomatic pelvic organ prolapse evaluated with PFDI20 and its subscales to report the prevalence of lower gastrointestinal symptoms and anal incontinence in the population of a public hospital and analyze its impact on quality of life. Methods: Cross-sectional study of patients with symptomatic POP. Patients were evaluated with demographic data, POP-Q, pelvic floor ultrasonography, urological parameters, and pelvic floor symptoms (PFDI-20), and quality of life (P-QoL) surveys. Patients were classified as CRADI-8 "positive" for colorectal symptoms, with responses "moderate" in at least 3 and/or "severe" in at least 2 of the items in the CRADI-8 questionnaires. Results: One hundred thirteen patients were included. 42.5% (48) were considered positive for colorectal symptoms on CRADI-8. 53.4% presented anal incontinence. No significant differences were found in sociodemographic variables, POP-Q stage, ultrasound parameters, or urological parameters. Positive patients had a significantly worse result in PFDI-20, POPDI (48 vs 28; p<0.001), UDI6 (51 vs 24; p<0.001), and in the areas of social limitation (44.4 vs 22.2; p = 0.045), sleep- energy (61.5 vs 44.4; p = 0.08), and severity (56.8 vs 43.7, p=0.015) according to P-QoL. Conclusion: Moderate or severe colorectal symptoms are seen in 40% of patients with symptomatic POP in our unit. Full evaluation of pelvic floor dysfunction symptoms should be performed routinely in urogynecology units.(FONIS SA12I2I53 - NCT02113969).

Asunto(s)

Incontinencia Fecal , Prolapso de Órgano Pélvico , Humanos , Femenino , Estudios Transversales , Incontinencia Fecal/epidemiología , Incontinencia Fecal/etiología , Persona de Mediana Edad , Prevalencia , Prolapso de Órgano Pélvico/epidemiología , Prolapso de Órgano Pélvico/complicaciones , Anciano , Calidad de Vida , Ginecología , Urología , Adulto , Atención Ambulatoria/estadística & datos numéricos

RESUMEN

Abstract Neospora caninum is a major cause of reproductive loss in cattle worldwide as it leads to abortion and animal repositioning. Although Toxoplasma gondii does not cause a reproductive problem in cattle, consuming raw or uncooked beef poses the risk of transmission. This study aimed to evaluate the occurrence of anti-N. caninum and anti-T. gondii antibodies in dairy cattle in the West and Northwest regions of São Paulo State, Brazil. A total of 653 serum samples from dairy cows were analyzed using an indirect immunofluorescence assay (IFA). Epidemiological data from the farms were associated with the serological results of the animals by logistic regression based on the presence of antibodies. The frequencies of the antibodies against N. caninum and T. gondii were 41.6% (272/653) and 11.5% (75/653), respectively. A statistically significant association was observed between: the serum anti-N. caninum antibodies and breed, history of food supplementation for calves, introduction of outside animals that later presented reproductive problems, and history of reproductive problems by the trimester of gestation. The present study highlights the importance of neosporosis in dairy cattle in the study regions and that the inclusion of this parasite in the investigation of animals with reproductive disorders is important.

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Resumo Neospora caninum é uma das principais causas de perda reprodutiva em bovinos em todo o mundo, pois leva ao aborto e ao reposicionamento animal. Embora o Toxoplasma gondii não cause problemas reprodutivos em bovinos, o consumo de carne bovina crua ou não cozida apresenta risco de transmissão. Este estudo teve como objetivo avaliar a ocorrência de anticorpos anti-N. caninum e anti-T. gondii em bovinos leiteiros nas regiões Oeste e Noroeste do Estado de São Paulo, Brasil. Um total de 653 amostras de soro de bovinos leiteiros, foi analisado utilizando-se o ensaio de imunofluorescência indireta (IFI). Os dados epidemiológicos das propriedades foram associados aos resultados sorológicos dos animais por meio de regressão logística. As frequências dos anticorpos contra N. caninum e T. gondii foram 41,6% (272/653) e 11,5% (75/653), respectivamente. Foi observada associação estatisticamente significativa entre: soropositividade para N. caninum e raça, histórico de suplementação alimentar para bezerros, introdução de animais externos que, posteriormente, apresentaram problemas reprodutivos e histórico de problemas reprodutivos no segundo trimestre de gestação. O presente estudo destaca a importância da neosporose em bovinos leiteiros, nas regiões de estudo, e a inclusão deste parasita na investigação de animais com distúrbios reprodutivos é fundamental.

RESUMEN

Abstract Objective: To analyze data of patients with symptomatic pelvic organ prolapse evaluated with PFDI20 and its subscales to report the prevalence of lower gastrointestinal symptoms and anal incontinence in the population of a public hospital and analyze its impact on quality of life. Methods: Cross-sectional study of patients with symptomatic POP. Patients were evaluated with demographic data, POP-Q, pelvic floor ultrasonography, urological parameters, and pelvic floor symptoms (PFDI-20), and quality of life (P-QoL) surveys. Patients were classified as CRADI-8 "positive" for colorectal symptoms, with responses "moderate" in at least 3 and/or "severe" in at least 2 of the items in the CRADI-8 questionnaires. Results: One hundred thirteen patients were included. 42.5% (48) were considered positive for colorectal symptoms on CRADI-8. 53.4% presented anal incontinence. No significant differences were found in sociodemographic variables, POP-Q stage, ultrasound parameters, or urological parameters. Positive patients had a significantly worse result in PFDI-20, POPDI (48 vs 28; p<0.001), UDI6 (51 vs 24; p<0.001), and in the areas of social limitation (44.4 vs 22.2; p = 0.045), sleep- energy (61.5 vs 44.4; p = 0.08), and severity (56.8 vs 43.7, p=0.015) according to P-QoL. Conclusion: Moderate or severe colorectal symptoms are seen in 40% of patients with symptomatic POP in our unit. Full evaluation of pelvic floor dysfunction symptoms should be performed routinely in urogynecology units. (FONIS SA12I2I53 - NCT02113969).

Asunto(s)

Humanos , Calidad de Vida , Prolapso de Órgano Pélvico

Asunto(s)

Influenza Pandémica, 1918-1919 , Gripe Humana , Navíos , Humanos

Asunto(s)

Humanos , Navíos , Gripe Humana , Influenza Pandémica, 1918-1919

RESUMEN

Introducción: la insulinorresistencia (IR) en diabetes tipo 1 (DM1) se asocia a mayor riesgo de complicaciones. El gold standard para evaluar insulinosensibilidad (IS) es el clamp euglucémico-hiperinsulinémico, aunque no se utiliza en la práctica clínica. La tasa estimada de disposición de la glucosa (TeDG) ha sido validada para estimar IS en DM1. Objetivos: relacionar IR en DM1 (TeDG) con complicaciones macro y microvasculares en pacientes asistidos en un Servicio de Diabetología. Materiales y métodos: se realizó un estudio transversal. La TeDG fue calculada utilizando HbA1c, perímetro de cintura y presencia de hipertensión. Resultados: se incluyeron 446 pacientes, 57% hombres, edad promedio 38 años y 15 años de evolución de la enfermedad. Los pacientes en el tercil más bajo de TeDG (>IR) tenían mayor edad, tiempo de evolución, IMC, dosis de insulina, mayores niveles de LDL y TGC y peor control metabólico. Eran más sedentarios y tenían mayor indicación de estatinas, aspirina, IECA/ARAII y metformina. Además registraban mayor prevalencia de complicaciones microvasculares y enfermedad cardiovascular (p<0,05). En el análisis multivariado, estos pacientes tuvieron mayor riesgo de nefropatía (OR 3,37 [95% CI 1,92-5,90]) y de cualquier complicación de la diabetes (OR 2,44 [95% CI 1,41-4,24]. Conclusiones: una menor TeDG se asoció a mayor presencia de complicaciones y comorbilidades. Esta herramienta podría resultar útil para identificar pacientes con mayor riesgo de complicaciones a fin de implementar estrategias más agresivas para el control de los factores de riesgo

Asunto(s)

Resistencia a la Insulina , Diabetes Mellitus Tipo 1

RESUMEN

BACKGROUND: There is no consensus on the relationship between depressive symptoms (DS) and pelvic organ prolapse (POP) symptoms and quality of life (QoL). Our hypothesis was that women with DS and POP have worse symptoms and QoL than those without DS and POP. AIM: Our aim was to compare two groups of POP patients, those with depressive symptoms and those without, and evaluate the association of symptoms and QoL. MATERIALS AND METHODS: This planned report is part of a prospective study evaluating the impact of pessary use among symptomatic POP patients. Patients were evaluated by POP quantification (POP-Q), pelvic ultrasound (US), voiding diaries, stress test, pad test, Pelvic Floor Distress Inventory (PFDI-20), Prolapse QoL (P-QoL) and the Goldberg Health Questionnaire (GHQ-12) (psychological health screening for DS when score ≥5). A sample size of 78 patients was required to demonstrate a 50-point difference in the global PFDI-20 score with 80% power and 95% probability. RESULTS: Ninety-one women with POP were included. GHQ-12 was positive in 47 (51.6%) patients. No differences were found in POP-Q, pad and stress test between those with a positive GHQ-12 and those without. However, GHQ-12 was associated with higher PFDI-20 scores and higher scores in seven of nine P-QoL domains. GHQ-12 persisted as an independent risk factor for worse P-QoL scores after multivariable analysis. CONCLUSION: A 'positive' screening for DS was associated with worse PFDI-20 and P-QoL scores despite no difference in objective measurements. It may be that depressed patients interpret their symptoms differently.

Asunto(s)

Depresión/etiología , Trastornos del Suelo Pélvico/psicología , Prolapso de Órgano Pélvico/psicología , Calidad de Vida , Encuestas y Cuestionarios , Anciano , Estudios Transversales , Femenino , Humanos , Persona de Mediana Edad , Prolapso de Órgano Pélvico/diagnóstico por imagen , Prolapso de Órgano Pélvico/fisiopatología , Estudios Prospectivos , Ultrasonografía , Incontinencia Urinaria de Esfuerzo/etiología

Asunto(s)

Cromosomas Humanos Par 17 , Cromosomas Humanos Par 1 , Cromosomas Humanos Par 22 , Cromosomas Humanos Par 9 , Leucemia Mielógena Crónica BCR-ABL Positiva/genética , Proteínas Asociadas a Microtúbulos/genética , Mutagénesis Insercional , Proteínas de Fusión Oncogénica/genética , Proteínas Serina-Treonina Quinasas/genética , Translocación Genética , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapéutico , Exones , Femenino , Proteínas de Fusión bcr-abl , Humanos , Hibridación Fluorescente in Situ , Leucemia Mielógena Crónica BCR-ABL Positiva/diagnóstico , Leucemia Mielógena Crónica BCR-ABL Positiva/tratamiento farmacológico , Persona de Mediana Edad , Análisis de Secuencia de ADN , Resultado del Tratamiento

RESUMEN

BACKGROUND: Malaria caused by Plasmodium vivax is a major public health problem worldwide that affects 70-80 million people in the Middle East, Asia, Western Pacific, South America and the Caribbean. Despite its epidemiological importance, few antigens from this parasite species have been characterized to date compared to Plasmodium falciparum, due in part to the difficulties of maintaining an in vitro culture of P. vivax. This study describes the identification of the P. falciparum thrombospondin-related apical merozoite protein homologue in P. vivax (PvTRAMP) and examines its potential to be further evaluated as vaccine candidate. METHODS: The gene encoding PvTRAMP was identified through an extensive search of the databases hosting the genome sequence of P. vivax. Genes adjacent to pvtramp were identified in silico to determine the degree of similarity between the protein sequences encoded by equivalent chromosomic fragments in P. falciparum and Plasmodium knowlesi. The pvtramp gene was amplified from cDNA of P. vivax schizont stages, cloned and expressed in Escherichia coli. Anti-PvTRAMP antisera was obtained by inoculating rabbits with PvTRAMP B cell epitopes produced as synthetic peptides in order to assess its recognition in parasite lysates by Western blot and in intact parasites by indirect immunofluorescence. The recognition of recombinant PvTRAMP by sera from P. vivax-infected individuals living in endemic areas was also assessed by ELISA. RESULTS: The PfTRAMP homologue in P. vivax, here denoted as PvTRAMP, is a 340-amino-acid long antigen encoded by a single exon that could have a potential role in cytoadherence, as indicated by the presence of a thrombospondin structural homology repeat (TSR) domain. According to its transcription and expression profile, PvTRAMP is initially located at the parasite's apical end and later on the parasite surface. Recombinant PvTRAMP is recognized by sera from infected patients, therefore, indicating that it is targeted by the immune system during a natural infection with P. vivax. CONCLUSIONS: The results of this work support conducting further studies with PvTRAMP to evaluate its immunogenicity and protection-inducing ability in the Aotus animal model.

Asunto(s)

Antígenos de Protozoos/genética , Antígenos de Superficie/genética , Merozoítos , Plasmodium vivax/genética , Proteínas Protozoarias/genética , Animales , Anticuerpos Antiprotozoarios/sangre , Anticuerpos Antiprotozoarios/inmunología , Antígenos de Protozoos/inmunología , Antígenos de Superficie/inmunología , Western Blotting , Clonación Molecular , Colombia , Biología Computacional , ADN Complementario/genética , ADN Complementario/aislamiento & purificación , ADN Protozoario/genética , ADN Protozoario/aislamiento & purificación , Ensayo de Inmunoadsorción Enzimática , Escherichia coli , Expresión Génica , Humanos , Vacunas contra la Malaria/inmunología , Microscopía Fluorescente , Proteínas Protozoarias/inmunología , Conejos , Homología de Secuencia de Aminoácido , Trombospondinas/genética

RESUMEN

Las reacciones cutáneas son las reacciones adversas medicamentosas más frecuentes ocurriendo en el 2 por ciento de todos los tratamientos. El objetivo de este trabajo es realizaar una revisión de los medicamentos que con mayor frecuencia causan el síndrome de Stevens-Johnson, la necrólisis epidérmica tóxica y señalar sus manifestaciones clínicas basados en la literatura y en los reportes del sistema nacional de farmacovigilancia. Especialmente nos interesa señalar estos síndromes como un problema relacionado con medicamentos, ya que algunos fármacos, muchos de ellos de uso prevalente, son el agente etiológico en la mayoría de los casos. La suspensión de aquellos no dedstinados a sostener una función vital es prioritaria ante la sospecha de una reacción cutánea severa. existen síntomas y signos que permiten al médico identificar los casos potencialmente graves. Los compuestos asociados con este tipo de reacciones sólo pueden ser identificados si los clínicos sospechan que existe una relación causa-efecto y posteriormente la informan. Es difícil conocer todo acerca de todos los medicamentos que prescribimos. Podemos acercarnos a esa meta si seleccionamos unos pocos para nuestra práctica clínica habitual. (AU)

Asunto(s)

Síndrome de Stevens-Johnson/inducido químicamente , Síndrome de Stevens-Johnson

RESUMEN

Las reacciones cutáneas son las reacciones adversas medicamentosas más frecuentes ocurriendo en el 2 por cientode todos los tratamientos. El objetivo de este trabajo es realizaar una revisión dee los medicamentos que con mayor frecuencia causan el síndrome de Stevens-Johnson, la necrólisis epidérmica tóxica y señalar sus manifestaciones clínicas basados en la literatura y en los reportes del sistema nacional de farmacovigilancia. Especialmente nos interesa señalar estos síndromes como un problema relacionado con medicamentos, ya que algunos fármacos, muchos de ellos de uso prevalente, so el agente tiológico en la mayoría de los casos. La suspensión de aquellos no dedstinados a sostener una función vital es prioritaria ante la sospecha de una reacción cutánea severa. existen síntomas y signos que permiten al médico identificar los casos potencialmente graves. Los compuestos asociados con este tipo de reacciones sólo pueden ser identificados si los clínicos sospechan que existe una relación causa-efecto y posteriormente la informan. Es difícil conocer todo acerca de todos los medicamentos que prescribimos. Podemos acercarnos a esa meta si seleccionamos unos pocos para nuestra práctica clínica habitual.

Asunto(s)

Síndrome de Stevens-Johnson , Síndrome de Stevens-Johnson/inducido químicamente

RESUMEN

474 milking herds from the north region of Paraná State were analyzed in this study. The herds were characterized for average productivity, genetic group, exploration and milking systems. Two milk samples (bulk or brass) were collected monthly from each farm, totalizing 21.957 samples. They were processed to determine fat, protein and somatic cells count (SCC) at Centralized Analyses Laboratory from Milking Herds Analyses Program of Paraná State. The data was submitted to analysis of variance (proc glm), regression analysis (proc reg) and correlation analysis (proc corr) in SAS to determine the influence of environmental and racial factors on SCC and milk composition, as well as SCC influence on protein and fat levels. Except for the milk system (p > 0.05), all other studied effects were highly significant (p 0.0001) on SCC. For the studied effects on protein and fat, the only one showing no significance (p > 0.05) was the herd average production, on protein level. The effect of SCC on milk protein and fat was statistically relevant (p 0.0001). With the increase of SCC, there was a reduction in protein level and an increase in fat level, although only 0.53% and 3.02% of the variation of these solids, respectively, can be credited to SCC.

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Foram analisados 474 rebanhos de bovinos leiteiros da região Norte do Paraná. Os rebanhos foram caracterizados quanto à produtividade média, ao grupo genético predominante, ao sistema de exploração e de ordenha utilizado. De cada propriedade foram colhidas mensalmente duas amostras compostas de leite, do tanque ou do latão, perfazendo um total de 21.957, para a determinação dos teores de gordura, de proteína e de contagem de células somáticas (CCS), no Laboratório de Análises Centralizadas do Programa de Análise de Rebanhos Leiteiros do Paraná. Com o objetivo de determinar a influência de fatores ambientais e raciais na CCS e constituintes do leite, bem como a influência da CCS sobre os teores de proteína e de gordura, os dados foram submetidos à análise de variância pelo método dos quadrados mínimos (proc glm), análise de regressão (proc reg) e análise de correlação (proc corr), no pacote estatístico SAS. Os efeitos estudados para a CCS mostraram-se altamente significativos (p 0,0001), com exceção do sistema de ordenha (p 0,05). Dos efeitos estudados para a proteína e para a gordura, a produção do rebanho não mostrou efeito significativo sobre o teor de proteína (p>0,05). O efeito da CCS sobre os teores de proteína e de gordura láctea foi estatisticamente relevante (p 0,0001). Com o aumento da CCS, ocorreu diminuição no teor de proteína e aumento no teor de g

RESUMEN

474 milking herds from the north region of Paraná State were analyzed in this study. The herds were characterized for average productivity, genetic group, exploration and milking systems. Two milk samples (bulk or brass) were collected monthly from each farm, totalizing 21.957 samples. They were processed to determine fat, protein and somatic cells count (SCC) at Centralized Analyses Laboratory from Milking Herds Analyses Program of Paraná State. The data was submitted to analysis of variance (proc glm), regression analysis (proc reg) and correlation analysis (proc corr) in SAS to determine the influence of environmental and racial factors on SCC and milk composition, as well as SCC influence on protein and fat levels. Except for the milk system (p > 0.05), all other studied effects were highly significant (p 0.0001) on SCC. For the studied effects on protein and fat, the only one showing no significance (p > 0.05) was the herd average production, on protein level. The effect of SCC on milk protein and fat was statistically relevant (p 0.0001). With the increase of SCC, there was a reduction in protein level and an increase in fat level, although only 0.53% and 3.02% of the variation of these solids, respectively, can be credited to SCC.

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Foram analisados 474 rebanhos de bovinos leiteiros da região Norte do Paraná. Os rebanhos foram caracterizados quanto à produtividade média, ao grupo genético predominante, ao sistema de exploração e de ordenha utilizado. De cada propriedade foram colhidas mensalmente duas amostras compostas de leite, do tanque ou do latão, perfazendo um total de 21.957, para a determinação dos teores de gordura, de proteína e de contagem de células somáticas (CCS), no Laboratório de Análises Centralizadas do Programa de Análise de Rebanhos Leiteiros do Paraná. Com o objetivo de determinar a influência de fatores ambientais e raciais na CCS e constituintes do leite, bem como a influência da CCS sobre os teores de proteína e de gordura, os dados foram submetidos à análise de variância pelo método dos quadrados mínimos (proc glm), análise de regressão (proc reg) e análise de correlação (proc corr), no pacote estatístico SAS. Os efeitos estudados para a CCS mostraram-se altamente significativos (p 0,0001), com exceção do sistema de ordenha (p 0,05). Dos efeitos estudados para a proteína e para a gordura, a produção do rebanho não mostrou efeito significativo sobre o teor de proteína (p>0,05). O efeito da CCS sobre os teores de proteína e de gordura láctea foi estatisticamente relevante (p 0,0001). Com o aumento da CCS, ocorreu diminuição no teor de proteína e aumento no teor de g

Asunto(s)

Humanos , Masculino , Femenino , Neurofarmacología/clasificación , Neurofarmacología/métodos , Ataque Isquémico Transitorio , Ataque Isquémico Transitorio/clasificación , Ataque Isquémico Transitorio/diagnóstico , Ataque Isquémico Transitorio/terapia , Epilepsia , Epilepsia/clasificación , Epilepsia/diagnóstico , Epilepsia/epidemiología , Epilepsia/terapia , Demencia/diagnóstico , Demencia/terapia , Enfermedad de Parkinson/diagnóstico , Enfermedad de Parkinson/terapia , Enfermedades del Nervio Óptico , Trastornos del Sueño-Vigilia/diagnóstico , Trastornos del Sueño-Vigilia/terapia