RESUMEN
The authors have developed enzyme immunoassay (EIA) for detecting antimeasles antibodies in the serum and compared it to routine methods. Preparation and purification of measles virus antigen used in EIA and optimal conditions of the reaction are described. Results of the routine methods and those of EIA correlated. EIA was used to determine titers of antimeasles antibodies in the sera of vaccinated children. 3-4 years after the vaccination the antibodies were undetectable in the sera of 23% of children.
Asunto(s)
Anticuerpos Antivirales/análisis , Virus del Sarampión/inmunología , Animales , Preescolar , Chlorocebus aethiops , Cobayas , Humanos , Técnicas para Inmunoenzimas , Virus del Sarampión/ultraestructura , Métodos , Microscopía Electrónica , Conejos , Células VeroRESUMEN
Venezuelan equine encephalomyelitis (VEE) virus-based vectors for expression of heterologous genes have been constructed using full-length cDNA copy of the interstrain recombinant virus (Trinidad Donkey and 230 strain). Gene cassettes carrying the subgenomic mRNA promoter of VEE virus and the preS2-S gene of hepatitis B virus (HBV) were inserted before or after the genes of structural viral proteins. Live virus stocks were obtained by transfection of chick embryo fibroblasts with in vitro transcribed full-length RNA. Insertions of gene cassettes before the structural region resulted in expression of HBsAg (VEHB-25 and VEHB-361 viruses), whereas insertions in the 3' region did not. Recombinant virus VEHB-25 expressed HBsAg during 5 passages in Vero cells. VEHB-25 stimulated immune response to HBsAg and was less virulent than the parental virus.
Asunto(s)
Encefalomielitis Equina Venezolana/genética , Antígenos de Superficie de la Hepatitis B/genética , Virus de la Hepatitis B/genética , Precursores de Proteínas/genética , Proteínas del Envoltorio Viral/genética , Animales , Línea Celular , Embrión de Pollo , Chlorocebus aethiops , Clonación Molecular , Encefalomielitis Equina Venezolana/inmunología , Antígenos de Superficie de la Hepatitis B/ultraestructura , Microscopía Electrónica , Plásmidos , Regiones Promotoras Genéticas , Precursores de Proteínas/ultraestructura , ARN Mensajero/genética , ARN Viral/genética , Conejos , Recombinación Genética , Pase Seriado , Células VeroRESUMEN
Clinical trials aimed at decreasing the terms of treatment and temporary disability in various categories of new cases of tuberculosis of the respiratory organs were conducted. It was shown to be possible at the account of decreasing the hospitalization period and increasing the role of controlled outpatient therapy.