RESUMEN

Rubber tree (Hevea brasiliensis) is the main feedstock for commercial rubber; however, its long vegetative cycle has hindered the development of more productive varieties via breeding programs. With the availability of H. brasiliensis genomic data, several linkage maps with associated quantitative trait loci have been constructed and suggested as a tool for marker-assisted selection. Nonetheless, novel genomic strategies are still needed, and genomic selection (GS) may facilitate rubber tree breeding programs aimed at reducing the required cycles for performance assessment. Even though such a methodology has already been shown to be a promising tool for rubber tree breeding, increased model predictive capabilities and practical application are still needed. Here, we developed a novel machine learning-based approach for predicting rubber tree stem circumference based on molecular markers. Through a divide-and-conquer strategy, we propose a neural network prediction system with two stages: (1) subpopulation prediction and (2) phenotype estimation. This approach yielded higher accuracies than traditional statistical models in a single-environment scenario. By delivering large accuracy improvements, our methodology represents a powerful tool for use in Hevea GS strategies. Therefore, the incorporation of machine learning techniques into rubber tree GS represents an opportunity to build more robust models and optimize Hevea breeding programs.

Asunto(s)

RESUMEN

The objective of this study was to evaluate the genetic variability of natural rubber latex traits among 44 elite genotypes of the rubber tree [Hevea brasiliensis (Willd. ex Adr. de Juss.) Müell. Arg.]. Multivariate analysis and machine learning techniques were used, targeting the selection of parents that demonstrate superior characters. We analyzed traits related to technological or physicochemical properties of natural rubber latex, such as Wallace plasticity (P0), the plasticity retention index [PRI (%)], Mooney viscosity (VR), ash percentage (Ash), acetone extract percentage (AE), and nitrogen percentage (N), to study genetic diversity. Multivariate [unweighted pair group method with arithmetic means (UPGMA) and Tocher)] and machine learning techniques [K-means and Kohonen's self-organizing maps (SOMs)] were employed. The genotypes showed high genetic variability for some of the evaluated traits. The traits PRI, Ash, and PO contributed the most to genetic diversity. The genotypes were classified into six clusters by the UPGMA method, and the results were consistent with the Tocher, K-means and SOM results. PRI can be used to improve the industrial potential of clones. The clones IAC 418 and PB 326 were the most divergent, followed by IAC 404 and IAC 56. These genotypes and others from the IAC 500 and 400 series could be used to start a breeding program. These combinations offer greater heterotic potential than the others, which can be used to improve components of rubber latex quality. Thus, it is important to consider the quality of rubber latex in the early stage of breeding programs.

Asunto(s)

RESUMEN

Rubber tree (Hevea brasiliensis) cultivation is the main source of natural rubber worldwide and has been extended to areas with suboptimal climates and lengthy drought periods; this transition affects growth and latex production. High-density genetic maps with reliable markers support precise mapping of quantitative trait loci (QTL), which can help reveal the complex genome of the species, provide tools to enhance molecular breeding, and shorten the breeding cycle. In this study, QTL mapping of the stem diameter, tree height, and number of whorls was performed for a full-sibling population derived from a GT1 and RRIM701 cross. A total of 225 simple sequence repeats (SSRs) and 186 single-nucleotide polymorphism (SNP) markers were used to construct a base map with 18 linkage groups and to anchor 671 SNPs from genotyping by sequencing (GBS) to produce a very dense linkage map with small intervals between loci. The final map was composed of 1,079 markers, spanned 3,779.7 cM with an average marker density of 3.5 cM, and showed collinearity between markers from previous studies. Significant variation in phenotypic characteristics was found over a 59-month evaluation period with a total of 38 QTLs being identified through a composite interval mapping method. Linkage group 4 showed the greatest number of QTLs (7), with phenotypic explained values varying from 7.67 to 14.07%. Additionally, we estimated segregation patterns, dominance, and additive effects for each QTL. A total of 53 significant effects for stem diameter were observed, and these effects were mostly related to additivity in the GT1 clone. Associating accurate genome assemblies and genetic maps represents a promising strategy for identifying the genetic basis of phenotypic traits in rubber trees. Then, further research can benefit from the QTLs identified herein, providing a better understanding of the key determinant genes associated with growth of Hevea brasiliensis under limiting water conditions.

RESUMEN

The rubber tree [Hevea brasiliensis (Willd. ex Adr. de Juss.) Muell. Arg.] is the only plant species worldwide that is cultivated for the commercial production of natural rubber. This study describes the genetic diversity of the Hevea spp. complex that is available in the main ex situ collections of South America, including Amazonian populations that have never been previously described. Genetic data were analyzed to determine the genetic structure of the wild populations, quantify the allelic diversity and suggest the composition of a core collection to capture the maximum genetic diversity within a minimal sample size. A total of 1,117 accessions were genotyped with 13 microsatellite markers. We identified a total of 408 alleles, 319 of which were shared between groups and 89 that were private in different groups of accessions. In a population structure and principal component analysis, the level of clustering reflected a primary division into the following two subgroups: cluster 1, which consisted of varieties from the advanced breeding germplasm that originated from the Wickham and Mato Grosso accessions; and cluster 2, which consisted of the wild germplasm from the Acre, Amazonas, Pará and Rondônia populations and Hevea spp. The analyses revealed a high frequency of gene flow between the groups, with the genetic differentiation coefficient (GST) estimated to be 0.018. Additionally, no distinct separation among the H. brasiliensis accessions and the other species from Amazonas was observed. A core collection of 99 accessions was identified that captured the maximum genetic diversity. Rubber tree breeders can effectively utilize this core collection for cultivar improvement. Furthermore, such a core collection could provide resources for forming an association panel to evaluate traits with agronomic and commercial importance. Our study generated a molecular database that should facilitate the management of the Hevea germplasm and its use for subsequent genetic and genomic breeding.

Asunto(s)

RESUMEN

Sugarcane species are allogamous, i.e. selfing is expected to reduce plant vigor. This study was undertaken to obtain and evaluate first-generation inbred lines that may be used for further crosses and determine the extent of inbreeding depression in sugarcane. In this research, 26 parental varieties were self-pollinated to obtain about 19,000 seedlings. Results showed that it is possible to segregate different traits in a given variety and find lines with promising outcome for all assessed characteristics. The soluble solids segregation observed in low and even high-Brix varieties increases the possibility of quick improvement in this trait. Self-pollination did not produce a general loss in yield traits such as soluble solids, stalk number and stalk diameter in any of the 26 varieties, but both stalk height and stalk weight showed strong inbreeding depression. Selfing-derived clones can be used to produce hybrid vigor in crossings.

Cana-de-açúcar tem sido considerada como uma planta alógama e, por esta razão, a autofecundação deveria produzir uma redução no vigor das plantas. O objetivo deste estudo foi obter e avaliar a primeira geração de linhas autofecundadas que podem ser usadas em mais cruzamentos e também determinar a extensão da depressão causada por essa autofecundação em cana-de-açúcar. Neste trabalho, 26 variedades utilizadas como parentais foram autofecundadas, das quais se obtiveram cerca de 19.000 plântulas. Os resultados mostraram que é possível segregar diferentes características em uma dada variedade sem perder vigor e encontrar progênies com características superiores. A segregação observada para Brix por cento em variedades de baixo, e até mesmo nas de alto Brix, aumenta a possibilidade de melhoramento dessa característica. Autofecundação não produziu uma perda geral nos atributos de produção nas 26 variedades estudadas, mas a altura e massa de colmos apresentaram forte depressão por endogamia. Clones derivados de autofecundação podem ser usados para produzir vigor híbrido nos cruzamentos.

RESUMEN

Genetic diversity of 60 Hevea genotypes, consisting of Asiatic, Amazonian, African and IAC clones, and pertaining to the genetic breeding program of the Agronomic Institute (IAC), Brazil, was estimated. Analyses were based on phenotypic multivariate parameters and microsatellites. Five agronomic descriptors were employed in multivariate procedures, such as Standard Euclidian Distance, Tocher clustering and principal component analysis. Genetic variability among the genotypes was estimated with 68 selected polymorphic SSRs, by way of Modified Rogers Genetic Distance and UPGMA clustering. Structure software in a Bayesian approach was used in discriminating among groups. Genetic diversity was estimated through Nei's statistics. The genotypes were clustered into 12 groups according to the Tocher method, while the molecular analysis identified six groups. In the phenotypic and microsatellite analyses, the Amazonian and IAC genotypes were distributed in several groups, whereas the Asiatic were in only a few. Observed heterozygosity ranged from 0.05 to 0.96. Both high total diversity (H T' = 0.58) and high gene differentiation (Gst' = 0.61) were observed, and indicated high genetic variation among the 60 genotypes, which may be useful for breeding programs. The analyzed agronomic parameters and SSRs markers were effective in assessing genetic diversity among Hevea genotypes, besides proving to be useful for characterizing genetic variability.

RESUMEN

Twenty two open-pollinated Hevea progenies from different parental clones of the Asian origin were tested at five sites in the Northwestern São Paulo State Brazil to investigate the progeny girth growth, rubber yield, bark thickness and plant height. Except for the rubber yield, the analysis of variance indicated highly significant (p<0.01) genotype x environment interaction and heterogeneity of regressions among the progenies. However, the regression stability analysis identified only a few interacting progenies which had regression coefficients significantly different from the expected value of one. The linear regressions of the progeny mean performance at each test on an environmental index (mean of all the progenies in each test) showed the general stability and adaptability of most selected Hevea progenies over the test environments. The few progenies which were responsive and high yielding on different test sites could be used to maximize the rubber cultivars productivity and to obtain the best use of the genetically improved stock under different environmental conditions.

Vinte e duas progênies de Hevea de polinização aberta obtidas de diferentes clones fenotipicamente selecionados de uma população de origem asiática Hevea brasiliensis (Willd. Adr. ex Juss.) Muell-Arg. durante três anos foram testadas em cinco locais. As variáveis perímetro do caule, produção de borracha, espessura da casca e a altura de planta foram determinadas em todos locais no Estado de São Paulo, Brasil. De acordo com as análises de variância, com exceção a produção de borracha, observou-se a existência de interações significativas entre genótipo x ambiente (p<0.01) e heterogeneidade de regressões entre as progênies. Porém, a análise de estabilidade de regressão identificou a interação de poucas progênies cujos coeficientes de regressão foram significativamente diferentes do valor esperado de um. Regressões lineares do desempenho médio das progênies para cada teste em um índice ambiental (média de todas as progênies em cada local) mostrou a estabilidade e adaptabilidade da grande maioria das progênies de Hevea nos diferentes locais. As poucas progênies que foram superiores e altamente produtivas nos diferentes testes de progênies podem ser utilizadas para maximizar a produtividade de cultivares de seringueira e determinar a melhor utilização do ganho genético dos grupos sob as diferentes condições ambientais de cultivo.

RESUMEN

The objective of this study was to assess genotype-year interaction and determine temporal stable genotypes across six years of rubber yield evaluation. Stability analyses were performed by Eberhart and Russell method for rubber yield. Twenty-five genotypes were analyzed in a randomized complete block design with three replications. The best genotype in one year was not same always in the other year. The genotype components were partitioned into linear (genotypes within year) and nonlinear (pooled deviations) components. Significant mean square for linear components was predictable. This indicated that the performance of genotypes across the years for rubber yield could be predicted. Among the analyzed genotypes the IAC 40 also was considered highly productive and vigorous, with suitable adaptation.

A interação genótipo x ano em culturas perenes como a seringueira Hevea brasiliensis (Willd. ex Adr. de Juss.) Muell.-Arg. representa o diferencial de resposta dos genótipos às mudanças climáticas anuais. O objetivo deste trabalho foi estudar a interação genótipo-ano e determinar genótipos temporalmente estáveis por meio da avaliação de seis anos de produção de borracha. Análises de estabilidade foram realizadas pelo método de Eberhart and Russell para produção de borracha na Estação Experimental de Votuporanga, região Noroeste do Estado de São Paulo, Brasil. Vinte e cinco genótipos foram analisados num delineamento de blocos ao acaso com três repetições. Os resultados mostraram que o melhor genótipo em um ano nem sempre foi o melhor em outro. Os componentes genéticos foram repartidos em lineares (genótipos dentro do ano) e não lineares (desvios agrupados). Quadrados médios significativos para os componentes lineares foram previsíveis indicando que o desempenho dos genótipos através dos anos em relação ao rendimento de borracha é passível de previsão. Entre os clones estudados o IAC 40 também foi considerado altamente produtivo e vigoroso, com adaptabilidade adequada.

RESUMEN

Analysis of variance and covariance was preformed on growth traits (stem girth, bark thickness, total height gain and rubber yield) of 22 open-pollinated progenies of the rubber tree Hevea brasiliensis from an Asian Hevea collection introduced to Agronomic Institute (Instituto Agronômico, Campinas, São Paulo, Brazil; IAC) in 1952. This progeny trial was replicated at three sites in São Paulo state and it was found that at three years from sowing there was statistically significant variation for girth, bark thickness, height and rubber yield. An individual test sites, values of individual plant heritability for girth ranged from h= 0.36 to h= 0.89 whereas values for heritability for progeny means ranged from h= 0.77 to h= 0.87. These moderate and high heritabilities suggest that a combination of progeny and within-progeny selection would be effective at increasing girth in this population at individual sites. Across sites, values of individual-plant heritability for girth ranged from h= 0.36 to h= 0.47, whereas values for heritability of progeny means girth ranged from h= 0.77 to h= 0.87. There were high positive genetic correlations between increased girth and bark thickness suggesting that breeding aimed at increasing girth would also increase bark thickness and possibly height

Asunto(s)

RESUMEN

In a field trial involving 68 rubber tree (Hevea spp.) clones calculation of genotypic correlation coefficients revealed significant age-age correlation from age 1 to 6 (immature period) for girth A and for age 7 to 12 (mature period) for girth B and for age 7 to 12 (production of latex) for yield. Rank correlation coefficients between all immature ages of girth (girth A), all ages of mature girth (girth B) and all annual rubber production (yield) were significant for the three traits, with the coefficients decreasing with increasing age. Selection of the sets of best 30, 15, 10 and 5 clones from the available 68 clones at a given age was generally accompanied by a descending order of percentage success. It was suggested: (a) to have the best 30 clones of age 6, select the set of best 36 clones at age 2, (b) to have the best 15 clones of age 6, select the set of best 20 clones at age 3, (c) to have the best 5 clones of age 6, select the set of best 8 clones at age 4, and (d) to have the best 3 clones of age 6, select the set of best 3 clones at age 5. More than 80% of the targeted clones on girth A or girth B basis and more than 76.7% clones on yield basis were found to get selected at steps (a) through (d). For achieving early multiplication of the most productive clone for deployment, multiplication should be started with the best 36 (i.e. 60%) clones selected at age 2.

Asunto(s)

RESUMEN

Six laticiferous system characters were investigated in 22 three-year-old, half-sib rubber tree [Hevea brasiliensis (Willd. ex Adr. de Juss.) Muell.-Arg.] progenies, evaluated at three sites (Votuporanga, Pindorama and Jaú, all in the São Paulo State, Brazil). The traits examined were: average rubber yield (Pp), average bark thickness (Bt), number of latex vessel rings (Lv), average distance between consecutive latex vessel rings (Dc), density of latex vessels per 5 mm per ring averaged over all rings (Dd) and the diameter of the latex vessels (Di). The joint analysis showed that site effect and progeny x sites interaction were significant for all traits, except Lv. Estimates of individual heritabilities across the three sites were high for Bt; moderate for Lv, Pp and Dc; low for Dd and very low for Di. Genetic correlations in the joint analysis showed high positive correlations between Pp and the other traits. Selecting the best five progenies would result in genetic gains of 24.91 percent for Pp while selecting best two plants within a progeny would result in a Pp genetic gain of 30.98 percent.

Asunto(s)

RESUMEN

The present paper deals with estimation of variance components, prediction of breeding values and selection in a population of rubber tree [Hevea brasiliensis (Willd. ex Adr. de Juss.) Müell.-Arg.] from Rio Branco, State of Acre, Brazil. The REML/BLUP (restricted maximum likelihood/best linear unbiased prediction) procedure was applied. For this purpose, 37 rubber tree families were obtained and assessed in a randomized complete block design, with three unbalanced replications. The field trial was carried out at the Experimental Station of UNESP, located in Selvíria, State of Mato Grosso do Sul, Brazil. The quantitative traits evaluated were: girth (G), bark thickness (BT), number of latex vessel rings (NR), and plant height (PH). Given the unbalanced condition of the progeny test, the REML/BLUP procedure was used for estimation. The narrow-sense individual heritability estimates were 0.43 for G, 0.18 for BT, 0.01 for NR, and 0.51 for PH. Two selection strategies were adopted: one short-term (ST - selection intensity of 8.85 percent) and the other long-term (LT - selection intensity of 26.56 percent). For G, the estimated genetic gains in relation to the population average were 26.80 percent and 17.94 percent, respectively, according to the ST and LT strategies. The effective population sizes were 22.35 and 46.03, respectively. The LT and ST strategies maintained 45.80 percent and 28.24 percent, respectively, of the original genetic diversity represented in the progeny test. So, it can be inferred that this population has potential for both breeding and ex situ genetic conservation as a supplier of genetic material for advanced rubber tree breeding programs.

Asunto(s)

RESUMEN

The best-yielding, best vigour and most stable Hevea clones are identified by growing clones in different environments. However, research on the stability in Hevea brasiliensis (Willd. Adr. ex Juss.) Muell.-Arg. is scarce. The objectives of this work were to assess genotype-environment interaction and determine stable genotypes. Stability analysis were performed on results for girth growth and rubber yield of seven clones from five comparative trials conducted over 10 years (girth growth) and four years (rubber yield) in São Paulo State, Brazil. Stability was estimated using the Eberhart and Russell (1966) method. Year by location and location variability were the dominant sources of interactions. The stability analysis identified GT 1 and IAN 873 as the most stable clones for girth growth and rubber yield respectively since their regression coefficients were almost the unity (beta = 1) and they had one of the lowest deviations from regressions (S2di). Their coefficient of determination (Rý) was as high as 89.5 percent and 89.8 percent confirming their stability. In contrast, clones such as PB 235, PR 261, and RRIM 701 for girth growth and clones such as GT 1 for rubber yield with regression coefficients greater than one were regarded as sensitive to environment changes.

Asunto(s)