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Aflatoxins are mycotoxins capable of contaminating food, and can cause toxic effects, teratogenicity, mutagenicity, carcinogenicity, and immune system depression. The presence of fungi which produce this metabolite in ingredients that compose the animal feed increased the risk of the emergence of aflatoxicosis. The objective of this study was to report an aflatoxicosis outbreak in rheas (Rhea americana) from a farm in Teresina, PI, Brazil, fed with commercial feed. After the deaths of four animals, epidemiological data were collected and complementary examinations were performed, including necropsy, histopathology, parasitology, blood culture, in addition to the analysis of the presence of toxins in the birds' feed. The diagnosis was based on the acute clinical signs, necropsy findings, which included carcass jaundice, an oedematous, yellowish, friable liver of increased size, and histopathological findings of moderate to severe congestion in all organs. The parasitological and microbiological tests performed were negative, not indicating systemic infectious causes. The analysis of the presence of mycotoxins revealed the existence of Aflatoxins B1, B2 and G1 in the commercial feed supplied, constituting a total of 66.89 µg/kg. This amount of aflatoxin exceeds the maximum level allowed by the Brazilian legislation. To our knowledge this is the first report of aflatoxicosis in rheas, and emphasizes the importance of the diagnosis, control, and prevention of mycotoxins in the quality of food provided to animals kept ex situ.

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Cães são importantes reservatórios para Leptospira spp, na transmissão humana e disseminação em ambientes urbanos. A infecção ocorre principalmente pelos sorovares Canicola e Icterohaemorrhaghiae, podendo ser infectados por outros sorovares incidentais. A manifestação pode ser desde uma forma assintomática a quadros clínicos graves, com síndromes ictérica, hemorrágica, urêmica e reprodutivas. Diversas técnicas estão disponíveis para o diagnostico, sendo realizado principalmente por meio da soroaglutinação microscópica (SAM), isolamento em cultura ou reação em cadeia da polimerase (PCR). O tratamento com antibióticos são eficazes na fase leptospirêmica e leptospirúrica, e, consequentemente, na prevenção e controle da disseminação da bactéria no ambiente.

Dogs are important reservoirs for Leptospira spp, human transmission and dissemination in urban environments. The infection occurs mainly by serovars Canicola and Icterohaemorrhaghiae, and can be infected by other incident serovars. A manifestation can be made asymptomatically in severe clinical conditions, with icteric, hemorrhagic, uremic and reproductive syndromes. Several techniques available for diagnosis, being carried out mainly by means of microscopic soroagglutination (MAT), isolation in culture or polymerase chain reaction (PCR). Treatment with antibiotics is initiated in the leptospiremic and leptospirurgical phase and, consequently, in the prevention and control of the spread of the bacteria in the environment.

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Cães são importantes reservatórios para Leptospira spp, na transmissão humana e disseminação em ambientes urbanos. A infecção ocorre principalmente pelos sorovares Canicola e Icterohaemorrhaghiae, podendo ser infectados por outros sorovares incidentais. A manifestação pode ser desde uma forma assintomática a quadros clínicos graves, com síndromes ictérica, hemorrágica, urêmica e reprodutivas. Diversas técnicas estão disponíveis para o diagnostico, sendo realizado principalmente por meio da soroaglutinação microscópica (SAM), isolamento em cultura ou reação em cadeia da polimerase (PCR). O tratamento com antibióticos são eficazes na fase leptospirêmica e leptospirúrica, e, consequentemente, na prevenção e controle da disseminação da bactéria no ambiente.(AU)

Dogs are important reservoirs for Leptospira spp, human transmission and dissemination in urban environments. The infection occurs mainly by serovars Canicola and Icterohaemorrhaghiae, and can be infected by other incident serovars. A manifestation can be made asymptomatically in severe clinical conditions, with icteric, hemorrhagic, uremic and reproductive syndromes. Several techniques available for diagnosis, being carried out mainly by means of microscopic soroagglutination (MAT), isolation in culture or polymerase chain reaction (PCR). Treatment with antibiotics is initiated in the leptospiremic and leptospirurgical phase and, consequently, in the prevention and control of the spread of the bacteria in the environment.(AU)

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Leptospirosis is a systemic disease caused by the species of bacteria Leptospira spp., which affects human beings, domestic and wild animals. The present study searched the presence of antibodies against Leptospira spp. in the canine population of the city Teresina, Piauí, and the most common serovars. Blood samples from 425 stray dogs were collected in the local zoonosis center in Teresina from July 2010 to January 2012 and submitted to the Microscopic Seroagglutination Test (MAT). This study found an average infection rate of 17.41% (IC 95%; 13,8 ­ 21,0) by 11 different serovars; the four most frequent were Canicola (18.9%), Autumnalis (16.2%), Icterohaemorrhagiae (12.1%), and Butembo (12.1%). The questions raised in this study indicated the occurrence of Leptospira spp infection in dogs of Terezina- Piaui, Brazil.(AU)

A leptospirose é uma doença sistêmica causada por bactéria Leptospira spp. que afeta seres humanos, animais domésticos e selvagens. O presente trabalho investigou a presença de anticorpos anti-Leptospira spp. na população canina da cidade de Teresina-Piauí, e os respectivos sorovares predominantes. Amostras de sangue de 425 cães foram coletadas no Centro de Controle de Zoonoses de Teresina, no período de julho de 2010 a janeiro de 2012, e submetidas à prova de Soroaglutinação Microscópica (SAM). Este estudo encontrou uma taxa de infecção média com 17,41% (IC 95%; 13,8 ­ 21,0) e 11 sorovares reagentes, sendo os prevalentes Canicola (18,9%), Autumnalis (16,2%), Icterohaemorrhagiae (12,1%) e Butembo (12,1%). As questões levantadas neste estudo indicam a ocorrência de infecção por Leptospira spp em cães da cidade de Teresina-Piauí, Brasil.(AU)

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Leptospirosis is a systemic disease caused by the species of bacteria Leptospira spp., which affects human beings, domestic and wild animals. The present study searched the presence of antibodies against Leptospira spp. in the canine population of the city Teresina, Piauí, and the most common serovars. Blood samples from 425 stray dogs were collected in the local zoonosis center in Teresina from July 2010 to January 2012 and submitted to the Microscopic Seroagglutination Test (MAT). This study found an average infection rate of 17.41% (IC 95%; 13,8 ­ 21,0) by 11 different serovars; the four most frequent were Canicola (18.9%), Autumnalis (16.2%), Icterohaemorrhagiae (12.1%), and Butembo (12.1%). The questions raised in this study indicated the occurrence of Leptospira spp infection in dogs of Terezina- Piaui, Brazil.(AU)

A leptospirose é uma doença sistêmica causada por bactéria Leptospira spp. que afeta seres humanos, animais domésticos e selvagens. O presente trabalho investigou a presença de anticorpos anti-Leptospira spp. na população canina da cidade de Teresina-Piauí, e os respectivos sorovares predominantes. Amostras de sangue de 425 cães foram coletadas no Centro de Controle de Zoonoses de Teresina, no período de julho de 2010 a janeiro de 2012, e submetidas à prova de Soroaglutinação Microscópica (SAM). Este estudo encontrou uma taxa de infecção média com 17,41% (IC 95%; 13,8 ­ 21,0) e 11 sorovares reagentes, sendo os prevalentes Canicola (18,9%), Autumnalis (16,2%), Icterohaemorrhagiae (12,1%) e Butembo (12,1%). As questões levantadas neste estudo indicam a ocorrência de infecção por Leptospira spp em cães da cidade de Teresina-Piauí, Brasil.(AU)

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This study aimed to search for anti-leptospira agglutinins in cattle from the Alvorada do GurguéiaFarm School, in the state of Piaui. Blood samples were collected from sixty-five bovines from various ages andboth sexes. The sorum was obtained by centrifugation of samples and sent to the Laboratory of Phisiopathologyof Animal Reproduction, located at the Campus Ministro Petrônio Portela, in Teresina – PI, where theserological diagnosis was conducted through the microscopic agglutination test by using a colletion of serovars.None of the samples were found reactive to any of the serovars used, what shows that bovine leptospirosis is not present at the area studied.

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ctBovine brucellosis, which is caused by Brucella abortus, is characterized for causing reproductivelosses. This study aimed to search for antibodies to Brucella abortus in cattle at the Alvorada do Gurguéia FarmSchool, in the state of Piaui, Brazil. Blood samples were collected from sixty five animals either male or femaleand from various ages. After serum was obtained by centrifugation of samples, it was sent to the Laboratory ofPhisiopathology of Animal Reproduction, located at the Campus Ministro Petrônio Portela, in Teresina – PI.There, it was conducted the serological diagnosis through the agglutination test by using the buffered acidifiedplate antigen (BAPA). All samples were found nonreactive to the test, which was possibly caused by the absenceof some factors that would favor the occurrence of brucellosis in the farm area. Thus, the results show thatbovine brucellosis is not present in the cattle of the studied area.

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Brucellosis is a disease of zoonotic character, of great global importance, caused by bacteria of thegenus Brucella that affects several animal species. In goats is caused by Brucella abortus and Brucellamellitensis, having as main implications abortion in females and epididymitis in males. The aim of this work wasresearch anti-Brucella abortus antibodies in the herd goats in the city of Bom Jesus, Piauí. Blood samples werecollected from 74 animals without definided breed. Obtaining the serum was accomplished at the ClinicalPathology Laboratory in Campus Professora Cinobelina Elvas - UFPI, the material was sent to thePhysiopathology of Animal reprocuction of Campus Ministro Petrônio Portela in Teresina- PI, where was heldthe serological diagnostic through Serum Agglutination test with Buffered Acid Antigen (AAT). The animalswere not reactive to the test, possibly because of some factors such as hygiene, lack of flooded areas and animalshave no contact with cattle.

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Brucellosis is an infectious disease of the zoonotic character, caused by bacteria of Brucella genus whichaffects several species. In sheep is caused by Brucella ovis, Brucella melitensis and Brucella abortus, resulting ineconomic losses, causing reproductive disorders such as epididymitis, orchitis and miscarriages. The objective ofthis work was research of agglutinins anti-Brucella abortus in sheeps of Colégio Técnico de Bom Jesus-PI. It wascollected 55 blood samples from animals of different ages to obtain the serum by centrifugation, held in thelaboratory of clinical pathology, Campus Professora Cinobelina Elvas - UFPI, the material was sent to the AnimalReproduction Pathophysiology Laboratory, Campus Ministro Petrônio Portela - UFPI, where conductedserological diagnosis through serum agglutination test Antigen with Buffered Acidified (AAT). The animals werenegative to the test, possibly due to the absence of some risk factors for the occurrence of the disease.

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ctBovine brucellosis, which is caused by Brucella abortus, is characterized for causing reproductivelosses. This study aimed to search for antibodies to Brucella abortus in cattle at the Alvorada do Gurguéia FarmSchool, in the state of Piaui, Brazil. Blood samples were collected from sixty five animals either male or femaleand from various ages. After serum was obtained by centrifugation of samples, it was sent to the Laboratory ofPhisiopathology of Animal Reproduction, located at the Campus Ministro Petrônio Portela, in Teresina PI.There, it was conducted the serological diagnosis through the agglutination test by using the buffered acidifiedplate antigen (BAPA). All samples were found nonreactive to the test, which was possibly caused by the absenceof some factors that would favor the occurrence of brucellosis in the farm area. Thus, the results show thatbovine brucellosis is not present in the cattle of the studied area.(AU)

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This study aimed to search for anti-leptospira agglutinins in cattle from the Alvorada do GurguéiaFarm School, in the state of Piaui. Blood samples were collected from sixty-five bovines from various ages andboth sexes. The sorum was obtained by centrifugation of samples and sent to the Laboratory of Phisiopathologyof Animal Reproduction, located at the Campus Ministro Petrônio Portela, in Teresina PI, where theserological diagnosis was conducted through the microscopic agglutination test by using a colletion of serovars.None of the samples were found reactive to any of the serovars used, what shows that bovine leptospirosis is not present at the area studied.(AU)

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Brucellosis is a disease of zoonotic character, of great global importance, caused by bacteria of thegenus Brucella that affects several animal species. In goats is caused by Brucella abortus and Brucellamellitensis, having as main implications abortion in females and epididymitis in males. The aim of this work wasresearch anti-Brucella abortus antibodies in the herd goats in the city of Bom Jesus, Piauí. Blood samples werecollected from 74 animals without definided breed. Obtaining the serum was accomplished at the ClinicalPathology Laboratory in Campus Professora Cinobelina Elvas - UFPI, the material was sent to thePhysiopathology of Animal reprocuction of Campus Ministro Petrônio Portela in Teresina- PI, where was heldthe serological diagnostic through Serum Agglutination test with Buffered Acid Antigen (AAT). The animalswere not reactive to the test, possibly because of some factors such as hygiene, lack of flooded areas and animalshave no contact with cattle.(AU)

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Brucellosis is an infectious disease of the zoonotic character, caused by bacteria of Brucella genus whichaffects several species. In sheep is caused by Brucella ovis, Brucella melitensis and Brucella abortus, resulting ineconomic losses, causing reproductive disorders such as epididymitis, orchitis and miscarriages. The objective ofthis work was research of agglutinins anti-Brucella abortus in sheeps of Colégio Técnico de Bom Jesus-PI. It wascollected 55 blood samples from animals of different ages to obtain the serum by centrifugation, held in thelaboratory of clinical pathology, Campus Professora Cinobelina Elvas - UFPI, the material was sent to the AnimalReproduction Pathophysiology Laboratory, Campus Ministro Petrônio Portela - UFPI, where conductedserological diagnosis through serum agglutination test Antigen with Buffered Acidified (AAT). The animals werenegative to the test, possibly due to the absence of some risk factors for the occurrence of the disease.(AU)

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Leptospirosis is a zoonotic disease which is a major public health problem, particularly in tropical and subtropical countries. This study aimed to investigate the presence of agglutinins anti-Leptospira dogs in the city of Bom Jesus, PI, using the agglutination test Microscopic Technique (SAM). Of the 106 samples, 20 (18.84%) were reactive. Of the 21 evaluated the following serovars were detected: Pyrogenes (43.47%), Canícola (39.13%), Icterohaemorrhagiae (8.69%) and Autumnalis (8.69%). It was concluded that leptospirosis is present in dogs in the city of Bom Jesus, PI, and the serovar most frequent was the Pyrogenes.

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Leptospirosis is a zoonotic disease which is a major public health problem, particularly in tropical and subtropical countries. This study aimed to investigate the presence of agglutinins anti-Leptospira dogs in the city of Bom Jesus, PI, using the agglutination test Microscopic Technique (SAM). Of the 106 samples, 20 (18.84%) were reactive. Of the 21 evaluated the following serovars were detected: Pyrogenes (43.47%), Canícola (39.13%), Icterohaemorrhagiae (8.69%) and Autumnalis (8.69%). It was concluded that leptospirosis is present in dogs in the city of Bom Jesus, PI, and the serovar most frequent was the Pyrogenes.(AU)

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O dengue é uma arbovirose considerada entre as prioritárias no mundo, e porser um sério problema de saúde pública com aumento do número de casos acada ano, resolveu-se realizar um estudo que descrevesse o perfilepidemiológico da doença no município de Teresina. Durante o períodocompreendido entre janeiro de 2003 e dezembro de 2007 foram notificadosmais de 10.000 casos de dengue clássica, com picos nos anos de 2003 e2007, sendo que neste último ano ocorreu o maior número de casos de febre hemorrágica do dengue. A doença na forma clássica predominou na faixaetária de 20 a 39 anos e na forma hemorrágica atingiu consideravelmentecrianças entre 5 e 9 anos. Indivíduos do sexo feminino foram os mais acometidos em ambas as formas da doença. A prevalência da doença foi expressiva no período estudado, sendo que em 2004 foi o ano mais brando, sem notificação da forma hemorrágica, fato observado em períodos pósepidemia da doença

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Os estudos sobre o motivo de eutanásia em centros de controle de zoonoses no Brasil são escassos. Foi realizada uma análise do relatório anual de 2006 na Gerência de Zoonoses (GEZOON) do município de Teresina (PI), com o objetivo de identificar quais os motivos da eutanásia de animais domésticos e o custo referente a este procedimento. Em 2006, 9.278 animais sofreram eutanásia, sendo que 91,9% (8.528) eram cães e 8% (750) gatos. A maioria dos animais que sofreram eutanásia foi proveniente de entrega voluntária. A entrega voluntária no GEZOON provém de proprietários que não querem mais permanecer com seus animais por motivos diversos. Tais eutanásias tiveram um alto custo para o contribuinte, o que pode inviabilizar o serviço. Nesse contexto, é urgente a adoção de medidas voltadas para a promoção da guarda responsável, como meio racional do uso dos recursos da saúde e controle das zoonoses endêmicas no município de Teresina. Além disso, o processo educativo deve também dedicar atenção para a questão ética de bem-estar animal.

Studies on the cause of euthanasia in animal disease control centers in Brazil are scarce. An analysis of the 2006 Annual Report on Zoonosis Management (GEZOON) in the city of Teresina (PI), in order to identify the reasons for euthanasia of domestic animals and the cost related to this procedure. In 2006, 9,278 animals were euthanized and 91.9% (8,528) were dogs and 8% (750) cats. Most euthanized animals were from voluntary surrender. The voluntary surrender in GEZOON comes from owners who no longer want to keep their pets for various reasons. Euthanasia had a high cost to taxpayers who can preclude the service. In this context, it is urgent to adopt measures that promote responsible ownership as a means of rational use of health resources and control of endemic zoonosis in the city of Teresina. Moreover, the educational process should also give attention to the ethical issue of animal welfare.

Los estudios sobre el motivo de eutanasia en centros de control de zoonosis en Brasil son escasos. Se realizó un análisis del informe anual de 2006 en la Gerencia de Zoonosis (GEZOON) del municipio de Teresina (PI), con el objetivo de identificar cuales los motivos de la eutanasia de animales domésticos y el costo referente a este procedimiento. En 2006, 9.278 animales sufrieron eutanasia, siendo que 91,9% (8.528) eran perros y 8% (750 gatos). La mayoría de los animales que sufrieron eutanasia fue proveniente de entrega voluntaria. La entrega voluntaria en el GEZOON viene de propietarios que no quieren más permanecer con sus animales por motivos diversos. Tales eutanasias tuvieron un alto costo para el contribuyente, lo que puede inviabilizar el trabajo. Por lo tanto, se hace urgente la adopción de medidas vueltas para la promoción de guardia responsable, como medio racional de uso de los recursos de salud y control de las zoonosis endémicas en el municipio de Teresina. Además, el proceso educativo debe también dedicar atención a la cuestión ética de bienestar del animal.

RESUMEN

The presence of anti leptospiral agglutinins (microscopic agglutination test - MAT) and DNA of leptospires was investigated in the kidney and urine (Polymerase Chain Reaction - PCR) in samples collected at the time of slaughter of cattle originating from the dairy basin of Parnaíba, Piauí, Brazil, as also the lesions in kidney, lung, liver, uterus, ovary and placenta (histopathology and immunohistochemistry). In the MAT, Hardjo was the predominant serovar with the highest number of reagent animals for the strain Hardjobovis/Sponselee. Anti-leptospiral antigens were scored in epithelial cells, interstitial vascular endothelium, endothelium of glomerular capillaries and Bowman's capsule of 20 positive animals. Inflammatory cells were more common in the kidney. PCR was positive in urine and kidney tissue.

Foi investigada a presença de aglutininas anti leptospiras (reação de soroaglutinação microscópica - SAM), de DNA de leptospiras no rim e na urina (reação de cadeia pela polimerase - PCR), bem como de lesões no rim, pulmão, fígado, útero, ovário e placenta (histopatologia e imunohistoquímica), em materiais colhidos, por ocasião do abate, de bovinos originários da bacia leiteira da região de Parnaíba, Piauí, Brasil. Na SAM o sorovar predominante foi o Hardjo com maior número de animais reagentes para a estirpe Hardjobovis/Sponselee. Antígenos anti leptospira foram marcados em 20 animais positivos nas células epiteliais e do endotélio vascular, endotélio dos capilares glomerulares e na cápsula de Bowman, somente nos animais infectados. O infiltrado inflamatório foi maior no rim do que nos demais órgãos. A PCR foi positiva em amostras de urina e tecido renal.

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The presence of anti leptospiral agglutinins (microscopic agglutination test - MAT) and DNA of leptospires was investigated in the kidney and urine (Polymerase Chain Reaction - PCR) in samples collected at the time of slaughter of cattle originating from the dairy basin of Parnaíba, Piauí, Brazil, as also the lesions in kidney, lung, liver, uterus, ovary and placenta (histopathology and immunohistochemistry). In the MAT, Hardjo was the predominant serovar with the highest number of reagent animals for the strain Hardjobovis/Sponselee. Anti-leptospiral antigens were scored in epithelial cells, interstitial vascular endothelium, endothelium of glomerular capillaries and Bowman's capsule of 20 positive animals. Inflammatory cells were more common in the kidney. PCR was positive in urine and kidney tissue.(AU)

Foi investigada a presença de aglutininas anti leptospiras (reação de soroaglutinação microscópica - SAM), de DNA de leptospiras no rim e na urina (reação de cadeia pela polimerase - PCR), bem como de lesões no rim, pulmão, fígado, útero, ovário e placenta (histopatologia e imunohistoquímica), em materiais colhidos, por ocasião do abate, de bovinos originários da bacia leiteira da região de Parnaíba, Piauí, Brasil. Na SAM o sorovar predominante foi o Hardjo com maior número de animais reagentes para a estirpe Hardjobovis/Sponselee. Antígenos anti leptospira foram marcados em 20 animais positivos nas células epiteliais e do endotélio vascular, endotélio dos capilares glomerulares e na cápsula de Bowman, somente nos animais infectados. O infiltrado inflamatório foi maior no rim do que nos demais órgãos. A PCR foi positiva em amostras de urina e tecido renal.(AU)

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As Leptospiroses são zoonoses graves de distribuição mundial que afetam o homem e outros animais. A infecção em animais, geralmente, é inaparente, ou os sintomas quando presentes são similares a outras infecções. Neste estudo foram colhidos soros de 119 ovinos e seus respectivos rins durante abate em feiras livres no município de Teresina-Piauí. Pela técnica de soroaglutinação microscópica (SAM) obtiveram-se 34 amostras sorológicas positivas para um ou mais sorovares de Leptospira spp., com taxa de ocorrência de 28,6 por cento de anticorpos anti-leptospiras, sendo 23 casos de infecção para um único sorovar e 11 com coaglutinações para dois ou mais sorovares. Dentre os sorovares patogênicos, o de maior ocorrência foi o Autumnalis (29,4 por cento). A análise histopatológica de 36 fragmentos de rins revelou alterações túbulo intersticiais em 33 (91,7 por cento) animais soro-reagentes. Lesões tubulares foram observadas em 20 (55,5 por cento) animais soro-reagentes. A presença de leptospiras, pela técnica de Warthin Starry, foi observada em 8 (22,20 por cento) amostras positivas. Pela técnica de imunoperixidase, de 20 casos analisados, foi verificada a presença de leptospira em 12 (60 por cento) de 20 amostras positivas. Nos animais soro-reagentes, o infiltrado inflamatório foi significantemente mais evidente na região córtico-medular e cortical do que na região medular (p=0,000), mas não houve diferença entre animais soro-reagentes e soro não-reagentes. Cilindros hialinos nos túbulos proximais estavam presentes em quantidade significantemente maior nos animais soro-reagentes comparados aos não-reagentes (p=0,0001). Em glomérulos, foi observada lesão discreta. Os resultados deste estudo mostram que ovinos soro-reagentes para leptospiras apresentam lesões renais túbulo intersticiais, com presença de leptospiras nos túbulos, o que confere a esses animais a condição de disseminadores da infecção.

Lepitospirosis is a serious worldwide distribution disease which affects man and other animals. The infection is generally asymptomatic in animals. In cases whose symptoms are present, symptoms are similar to other infections. In the present study serum samples from 119 sheep and their kidneys were collected during their slaughter in outdoor markets in the city of Teresina, Piauí, Brazil. The Microscopic Agglutination Test (MAT) obtained 34 positive serological samples for one or more Leptospira spp. serovar with occurrence rate of 28.6 percent of leptospiral antibodies. There were 23 cases of infection for a single serovar, and 11 cases with coagglutination for two or more serovars. Autumnalis had the highest occurrence (29.4 percent) among the pathogenic serovars. The histopathological analysis of 36 kidney fragments revealed tubulo-interstitial alterations in 33 (91.7 percent) positive animals. Tubular lesions were observed in 20 (55.5 percent) positive animals. The Warthin Starry staning revealed the presence of Leptospira in 8 (22.20 percent) of the 36 positive samples. The immunoperoxidase staining revealed the presence of Leptospira in 12 (60 percent) of 20 positive samples. The inflammatory infiltrate in the positive animals was significantly more evident in the cortical-medullar and cortical regions than in the medullar region (p=0.000), however, there was no difference between positive and negative animals. The presence of hyaline casts in the proximal tubules was significantly higher in positive animals compared to the negative ones (p=0.0001). Discrete lesion was observed in glomeruli. In conclusion, the results from this study showed that sheep which are positive for Leptospira present tubulo-intersticial renal lesions with the presence of Leptospira in the tubules, conferring to these animals the condition of asymptomatic carriers.