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Sci Rep ; 8(1): 798, 2018 01 15.
Artículo en Inglés | MEDLINE | ID: mdl-29335484

RESUMEN

Bacterial species are able to colonize and establish communities in biotic and abiotic surfaces. Moreover, within the past five decades, incidence of bacterial strains resistant to currently used antibiotics has increased dramatically. This has led to diverse health issues and economical losses for different industries. Therefore, there is a latent need to develop new and more efficient antimicrobials. This work reports an increased production of an exopolysaccharide in a native yeast strain isolated from the Mexican Northeast, Rhodotorula mucilaginosa UANL-001L, when co-cultured with E. coli. The exopolysaccharide produced is chemically and physically characterized and its applications as an antimicrobial and antibiofilm are explored. The exopolysaccharide is capable of inhibiting planktonic growth and biofilm formation in Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. Additionally, the exopolysaccharide studied here does not exhibit cytotoxic effects when assessed both, in vitro against an H9c2 mammalian cell line, and in vivo in a murine toxicity model. Taken together, the properties of this exopolysaccharide indicate that it has potential applications to inhibit bacterial colonization in medical and industrial settlings.


Asunto(s)
Antiinfecciosos/farmacología , Escherichia coli/crecimiento & desarrollo , Polisacáridos/biosíntesis , Rhodotorula/crecimiento & desarrollo , Animales , Apoptosis/efectos de los fármacos , Biopelículas/efectos de los fármacos , Línea Celular , Escherichia coli/efectos de los fármacos , Escherichia coli/metabolismo , Cinética , Microscopía Electrónica de Rastreo , Polisacáridos/química , Polisacáridos/farmacología , Pseudomonas aeruginosa/fisiología , Ratas , Rhodotorula/efectos de los fármacos , Rhodotorula/metabolismo , Espectroscopía Infrarroja por Transformada de Fourier , Staphylococcus aureus/fisiología
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