RESUMEN

Although enteropathogenic Escherichia coli (EPEC) are well-recognized diarrheal agents, their ability to translocate and cause extraintestinal alterations is not known. We investigated whether a typical EPEC (tEPEC) and an atypical EPEC (aEPEC) strain translocate and cause microcirculation injury under conditions of intestinal bacterial overgrowth. Bacterial translocation (BT) was induced in female Wistar-EPM rats (200-250 g) by oroduodenal catheterization and inoculation of 10 mL 10(10) colony forming unit (CFU)/mL, with the bacteria being confined between the duodenum and ileum with ligatures. After 2 h, mesenteric lymph nodes (MLN), liver and spleen were cultured for translocated bacteria and BT-related microcirculation changes were monitored in mesenteric and abdominal organs by intravital microscopy and laser Doppler flow, respectively. tEPEC (N = 11) and aEPEC (N = 11) were recovered from MLN (100%), spleen (36.4 and 45.5%), and liver (45.5 and 72.7%) of the animals, respectively. Recovery of the positive control E. coli R-6 (N = 6) was 100% for all compartments. Bacteria were not recovered from extraintestinal sites of controls inoculated with non-pathogenic E. coli strains HB101 (N = 6) and HS (N = 10), or saline. Mesenteric microcirculation injuries were detected with both EPEC strains, but only aEPEC was similar to E. coli R-6 with regard to systemic tissue hypoperfusion. In conclusion, overgrowth of certain aEPEC strains may lead to BT and impairment of the microcirculation in systemic organs.

Asunto(s)

Traslocación Bacteriana/fisiología , Escherichia coli Enteropatógena/fisiología , Infecciones por Escherichia coli/microbiología , Intestinos/microbiología , Microcirculación , Animales , Niño , Femenino , Humanos , Hígado/microbiología , Ganglios Linfáticos/microbiología , Mesenterio/microbiología , Ratas , Ratas Wistar , Bazo/microbiología

RESUMEN

Although enteropathogenic Escherichia coli (EPEC) are well-recognized diarrheal agents, their ability to translocate and cause extraintestinal alterations is not known. We investigated whether a typical EPEC (tEPEC) and an atypical EPEC (aEPEC) strain translocate and cause microcirculation injury under conditions of intestinal bacterial overgrowth. Bacterial translocation (BT) was induced in female Wistar-EPM rats (200-250 g) by oroduodenal catheterization and inoculation of 10 mL 10(10) colony forming unit (CFU)/mL, with the bacteria being confined between the duodenum and ileum with ligatures. After 2 h, mesenteric lymph nodes (MLN), liver and spleen were cultured for translocated bacteria and BT-related microcirculation changes were monitored in mesenteric and abdominal organs by intravital microscopy and laser Doppler flow, respectively. tEPEC (N = 11) and aEPEC (N = 11) were recovered from MLN (100 percent), spleen (36.4 and 45.5 percent), and liver (45.5 and 72.7 percent) of the animals, respectively. Recovery of the positive control E. coli R-6 (N = 6) was 100 percent for all compartments. Bacteria were not recovered from extraintestinal sites of controls inoculated with non-pathogenic E. coli strains HB101 (N = 6) and HS (N = 10), or saline. Mesenteric microcirculation injuries were detected with both EPEC strains, but only aEPEC was similar to E. coli R-6 with regard to systemic tissue hypoperfusion. In conclusion, overgrowth of certain aEPEC strains may lead to BT and impairment of the microcirculation in systemic organs.

Asunto(s)

Animales , Niño , Femenino , Humanos , Ratas , Traslocación Bacteriana/fisiología , Escherichia coli Enteropatógena/fisiología , Infecciones por Escherichia coli/microbiología , Intestinos/microbiología , Microcirculación , Hígado/microbiología , Ganglios Linfáticos/microbiología , Mesenterio/microbiología , Ratas Wistar , Bazo/microbiología

RESUMEN

AIMS: To determine the prevalence of Shiga toxin-producing Escherichia coli (STEC) and serotypes and virulence markers of the STEC isolates from beef and dairy cattle in Rio Grande do Sul, Brazil. METHODS AND RESULTS: Faecal samples from beef cattle were collected at slaughterhouses. The isolates were submitted to colony hybridization assay with specific DNA probes for stx1, stx2 and eae genes, and serotyped for the identification of O and H antigens. Thirty-nine per cent of beef cattle surveyed harboured at least one STEC strain. Among the distinct serotypes identified, 10 were shared by both beef and dairy cattle. Most of the strains isolated harboured stx2. Genotypic and phenotypic profiles allowed the identification of 34 and 31 STEC strains, isolated from beef and dairy cattle, respectively. Serotypes O10:H14, O15:H21, O96:H21, O119:H4, O124:H11, O128:H21, O137:H-, O141:H19, O159:H42, O160:H2 and O177:H11, identified in this study, have not been previously reported as STEC isolated from cattle. CONCLUSIONS: Cattle are an important reservoir of STEC strains associated with human diseases in South America. SIGNIFICANCE AND IMPACT OF THE STUDY: Determining the prevalence, genotypic profile and serotypes of STEC strains isolated from cattle enables the prediction of possible risk for public health.

Asunto(s)

Bovinos/microbiología , Escherichia coli/clasificación , Escherichia coli/patogenicidad , Toxinas Shiga/biosíntesis , Animales , Brasil , Industria Lechera , Reservorios de Enfermedades , Escherichia coli/aislamiento & purificación , Escherichia coli/metabolismo , Infecciones por Escherichia coli , Heces/microbiología , Humanos , Carne/microbiología , Serotipificación , Toxinas Shiga/genética , Factores de Virulencia/genética

RESUMEN

In order to determine the occurrence, serotypes and virulence markers of Shiga toxin-producing Escherichia coli (STEC) strains, 153 fecal samples of cattle randomly selected from six dairy farms in Sao Paulo State, Brazil, were examined for Shiga toxin (Stx) production by the Vero cell assay. Feces were directly streaked onto MacConkey Sorbitol Agar and incubated at 37 degrees C overnight. Sorbitol-negative colonies (maximum 20) and up to 10 sorbitol-positive colonies from each plate were subcultured onto presumptive diagnostic medium IAL. Sorbitol-negative isolates were screened with O157 antiserum for identification of O157:H7 E. coli. Isolates presenting cytotoxic activity were submitted to colony hybridization assays with specific DNA probes for stx1, stx2, eae, Ehly and astA genes. The isolation rate of STEC ranged from 3.8 to 84.6% depending on the farm analysed. STEC was identified in 25.5% of the animals, and most of them (64.1%) carried a single STEC serotype. A total of 202 STEC isolates were recovered from the animals, and except for the 2 O157:H7 isolates all the others expressed cytotoxic activity. The great majority of the STEC isolates carried both stx1 and stx2 genes (114/202, 56.4%) or stx2 (82/202, 40.6%); and whereas the Ehly sequence occurred in most of them (88%) eae was only observed in O157:H7 and O111:HNM isolates. Serotypes O113:H21, O178:H19 and O79:H14 were the most frequent STEC serotypes identified and widely distributed among animals from different farms, while others such as O77:H18, O88:H25 and O98:H17 occurred only in particular farms. This is the first report on the occurrence of STEC in dairy cattle in Sao Paulo State, and the results point to substantial differences in rate of isolation, serotypes and genetic profile of STEC that has been previously described among beef cattle in our community. Moreover, to our knowledge O79:H14 and O98:H17 represent new STEC serotypes, while O178:H19 has only been recently reported in Spain.

Asunto(s)

Enfermedades de los Bovinos/microbiología , Infecciones por Escherichia coli/veterinaria , Escherichia coli O157/clasificación , Toxina Shiga I/biosíntesis , Toxina Shiga II/biosíntesis , Adhesinas Bacterianas/genética , Adhesinas Bacterianas/metabolismo , Animales , Toxinas Bacterianas/genética , Toxinas Bacterianas/metabolismo , Brasil , Bovinos , ADN Bacteriano/química , ADN Bacteriano/genética , Industria Lechera , Infecciones por Escherichia coli/microbiología , Escherichia coli O157/genética , Escherichia coli O157/crecimiento & desarrollo , Escherichia coli O157/patogenicidad , Proteínas de Escherichia coli/genética , Proteínas de Escherichia coli/metabolismo , Heces/microbiología , Femenino , Hibridación de Ácido Nucleico , Antígenos O/metabolismo , Serotipificación , Toxina Shiga I/genética , Toxina Shiga II/genética

RESUMEN

Escherichia coli isolates recovered from 182 fecal specimens from dogs up to five months old from the cities of São Paulo and Campinas, SP, Brazil, were examined by polymerase chain reaction (PCR) for several virulence factors and properties. The eae gene was found in 23 isolates of E. coli from 22 dogs, 19 of 146 (13%) from dogs with diarrhea and 3 of 36 (8.3%) from dogs with no diarrhea. Two different eae+ isolates were recovered from one dog with diarrhea. Isolates from two dogs with diarrhea harbored the bfpA gene, and none of the isolates possessed genes for enterotoxins, the EAF plasmid or Shiga toxins. PCR showed that, among the 23 isolates, eight were positive for beta intimin, six for gamma, two for, one for alpha, one for kappa, and five showed no amplification with any of the nine pairs of specific intimin primers used. PCR also showed that the LEE (locus of enterocyte effacement) was inserted in selC in four isolates, likely in pheU in seven isolates, and in undetermined sites in twelve isolates. Fifteen isolates adhered to HEp-2 cells and were fluorescence actin staining (FAS) positive. The predominant adherence pattern was the localized adherence-like (LAL) pattern. The eae-positive isolates belonged to a wide diversity of serotypes, including O111:H25, O119:H2 and O142:H6, which are serotypes that are common among human EPEC. These results confirmed the presence of EPEC in dogs (DEPEC) with and without diarrhea. The virulence factors found in these strains were similar to those in human EPEC, leading to the possibility that EPEC may move back and forth among human and canine populations.

Asunto(s)

Adhesión Bacteriana , Enfermedades de los Perros/microbiología , Infecciones por Escherichia coli/veterinaria , Escherichia coli/patogenicidad , Adhesinas Bacterianas/genética , Animales , Secuencia de Bases , Brasil , Diarrea/microbiología , Diarrea/veterinaria , Perros , Escherichia coli/genética , Escherichia coli/aislamiento & purificación , Infecciones por Escherichia coli/microbiología , Proteínas de Escherichia coli/genética , Heces/microbiología , Proteínas Fimbrias/genética , Genes Bacterianos , Humanos , Reacción en Cadena de la Polimerasa/veterinaria , Serotipificación/veterinaria , Virulencia/genética

RESUMEN

Diarrheal diseases are a leading cause of childhood morbidity and mortality in Latin America. Most studies have focused on infants but not on older children. We enrolled 505 children (age, 12-59 months) with diarrhea and age-matched controls in a case-control study in Sao Paulo, Brazil. Independent risk factors for diarrhea included another household member with diarrhea (matched odds ratio [mOR], 8.1; attributable fraction [AF], 0.17; P<.001) and consumption of homemade juice (mOR, 1.8; AF, 0.10; P=.01); protective factors included boiling of the baby bottle or nipple (mOR, 0.60; AF, 0.19; P=.026), childcare at home (mOR, 0.58; AF, 0.12; P=.004), and piped sewage (mOR, 0.58; AF, 0.05; P=.047). Hand washing by the caretaker after helping the child defecate protected against Shigella infection (mOR, 0.35; P<.05). Preparation of rice, beans, or soup in the morning and serving it to children after noon were associated with enterotoxigenic Escherichia coli infection (mOR, 8.0; P<.05). In these poor households, 28% of cases of diarrhea in 1-4-year-old children was attributable to easily modifiable exposures.

Asunto(s)

Diarrea Infantil/epidemiología , Diarrea/epidemiología , Infecciones por Adenovirus Humanos/epidemiología , Infecciones por Adenovirus Humanos/prevención & control , Anciano , Brasil/epidemiología , Infecciones por Campylobacter/epidemiología , Infecciones por Campylobacter/prevención & control , Estudios de Casos y Controles , Preescolar , Diarrea/microbiología , Diarrea/prevención & control , Diarrea/virología , Diarrea Infantil/microbiología , Diarrea Infantil/prevención & control , Diarrea Infantil/virología , Disentería Bacilar/epidemiología , Disentería Bacilar/prevención & control , Infecciones por Escherichia coli/epidemiología , Infecciones por Escherichia coli/prevención & control , Heces/microbiología , Heces/virología , Femenino , Humanos , Lactante , Masculino , Selección de Paciente , Valor Predictivo de las Pruebas , Factores de Riesgo , Infecciones por Rotavirus/epidemiología , Infecciones por Rotavirus/prevención & control , Distribución por Sexo , Shigella/aislamiento & purificación , Shigella/patogenicidad , Especificidad de la Especie

RESUMEN

Twenty-nine Shiga toxin-producing Escherichia coli (STEC) strains were identified in a collection of 2,607 isolates from patients with diarrhea in São Paulo, Brazil, from 1976 to 1999. The STEC strains belonged mainly to serotypes O111:HNM (HNM, nonmotile) (13 of 29 [44.8%]), O111:H8 (7 of 29 [24%]), and O26:H11 (4 of 29 [13.8%]); stx(1) eae (26 of 29 [89.6%]), in combination with either enterohemorrhagic E. coli hlyA (11 of 26 [42%]) or astA (24 of 26 [92.3%]), prevailed. The O111 STEC strains were distinguished by their inability to decarboxylate lysine. The predominance of STEC O111 and O26 since the late 1970s and the identification of STEC serotypes O55:H19, O93:H19, and O118:H16 in association with human infections in Brazil are described for the first time.

Asunto(s)

Escherichia coli/genética , Toxina Shiga/genética , Virulencia , Técnicas de Tipificación Bacteriana , Brasil/epidemiología , Diarrea/microbiología , Escherichia coli/aislamiento & purificación , Infecciones por Escherichia coli/epidemiología , Escherichia coli O157/genética , Escherichia coli O157/aislamiento & purificación , Humanos , Serotipificación

RESUMEN

The frequency of diarrhoeagenic Escherichia coli (DEC) strains was investigated in 253 children up to 3 years old, with (patient group, PG, 199 children) and without (control group, CG, 54 children) diarrhoea, living in Rio de Janeiro, Brazil. DEC strains were detected in 70 (27.6%) children, including 54 (27.1%) with diarrhoea and 16 (29.6%) without diarrhoea. Enteroaggregative E. coli (EAEC) was the most frequent DEC category, accounting for 14.6% of the isolates in the PG and for 11.1% in the CG. E. coli strains carrying enteropathogenic E. coli (EPEC) virulence markers showed higher incidence in the CG (12.9%) than in the PG (8.0%). E. coli strains belonging to non-classical EPEC groups that carried eae only or eae and bfpA, designated as attaching-effacing E. coli (AEEC) were the most frequent (79.1%). Simultaneous presence of multiple EPEC virulence factors (EAF/eae/bfpA) were only detected among strains isolated from the PG. Enterotoxigenic E. coli (ETEC) strains were isolated from 5.5% of the children in the CG and from 3.5% of those in the PG. Most of the ETEC isolates were LT-probe positive (70%) and none carried both LT-I and ST-I probe sequences. One enteroinvasive E. coli (EIEC) strain was recovered from a child with diarrhoea. No stx-probe positive E. coli strains were detected. Overall, DEC strains were not found to be significantly associated with diarrhoea (p>0.05). However, the higher incidence of EAEC, the most frequent DEC category, among children with diarrhoea, suggests a potential role of EAEC as an important enteric pathogen in the community investigated.

Asunto(s)

Diarrea/microbiología , Infecciones por Escherichia coli/microbiología , Escherichia coli/clasificación , Pruebas de Aglutinación , Toxinas Bacterianas/genética , Toxinas Bacterianas/aislamiento & purificación , Brasil/epidemiología , Preescolar , ADN Bacteriano/química , ADN Bacteriano/genética , Diarrea/epidemiología , Enterotoxinas/genética , Enterotoxinas/aislamiento & purificación , Escherichia coli/genética , Escherichia coli/aislamiento & purificación , Escherichia coli/patogenicidad , Infecciones por Escherichia coli/epidemiología , Heces/microbiología , Femenino , Humanos , Incidencia , Lactante , Masculino , Hibridación de Ácido Nucleico , Virulencia

RESUMEN

A total of 102 Escherichia coli strains belonging to serogroups O127 and O142 were examined for genotypic and phenotypic characteristics. The most frequent serotypes found were O127:H21, O127:H40 and O142:H34. The virulence properties were evaluated by adhesion to HeLa cells and hybridization with gene probes for diarrhoeagenic E. coli. Most strains in the two serogroups were categorized as enteropathogenic E. coli, but enteroaggregative E. coli was also detected in both serogroups. All strains that carried the eae sequence presented the LEE region inserted in selC. Five ribotypes were detected in serogroup O127 and four in serogroup O142 and a correlation between serotypes and ribotypes was observed mainly in serogroup O142.

Asunto(s)

Diarrea Infantil/microbiología , Escherichia coli/clasificación , Escherichia coli/patogenicidad , Virulencia/genética , Brasil , Preescolar , Genotipo , Humanos , Lactante , Fenotipo

RESUMEN

Enteroaggregative Escherichia coli (EAEC) is defined by the ability to produce aggregative adherence (AA) to cultured cells. We analysed 128 EAEC strains, isolated from children with and without diarrhoea, regarding the presence of 11 EAEC virulence genes. Seventy strains carried and 58 lacked the EAEC probe sequence; 17 probe positive and 31 probe negative strains showed variations in the AA pattern. All EAEC probe positive strains carried at least one EAEC marker; aspU (94.3%), irp2 (91.4%), and aggR (74.3%) were the most prevalent. Conversely, among the EAEC probe negative strains, 41.4% were devoid of any marker and astA predominated (44.8%). No significant statistical difference in the prevalence of any marker between cases and controls in both EAEC probe groups or AA variants was found. We suggest that the EAEC probe positive strains may have a higher pathogenic potential or alternatively, EAEC probe negative strains may harbour virulence factors as yet undescribed.

Asunto(s)

Diarrea/microbiología , Escherichia coli/patogenicidad , Biomarcadores , Niño , Humanos , Sondas Moleculares , Virulencia/genética

Asunto(s)

Humanos , Sondas de ADN , Hibridación de Ácido Nucleico/métodos , Bacterias/genética , Sondas de ADN , Enfermedades Genéticas Congénitas/diagnóstico , Enfermedades Genéticas Congénitas/genética , Infecciones Bacterianas/diagnóstico , Virosis/diagnóstico , Virus/aislamiento & purificación

RESUMEN

The usefulness of plasmid profile analysis to differentiate strains of enteropathogenic Escherichia coli (EPEC) was evaluated by studying 123 strains of the most prevalent serotypes causing infant diarrhea in the city of Säo Paulo, Brazil, i. e.,0111ab:H-, 0111ab:H2 and 0119:H6. No common profiles were found among strains of distinct serotypes. However, within each serotype, most of the strains were grouped within a few major profiles. More than 68% of the strainsof serotypes 0111ab:H- and 0111ab:H2 were included in 6 and 9 major profiles, respectively. In 0119:H6, abouth 48% of the strainswere included in 3 major profiles. This analysis suggewsts that only a few EPEC clones are causing infant diarrhea in Säo Paulo and revealed that the distribution of serotypes 0111 ab:H- and 0111ab:H2 during the one-year study was at least partly determined by small outbreaks of the most common profiles. We conclude that plasmid profile analysis is very useful to differentiate strasins within specific EPEC serotypes

Asunto(s)

Diarrea Infantil/epidemiología , Escherichia coli , Plásmidos/análisis