RESUMEN
OBJECTIVE To identify risk factors related to Mycobacterium abscessus subsp. bolletii infection during an outbreak, associated with laparoscopic surgery and to propose recommendations for preventing new cases. DESIGN A retrospective cohort study. SETTING A private hospital in Manaus, Brazil. PATIENTS A cohort of 222 patients who underwent laparoscopic surgery between July 2009 and August 2010 by a single surgical team. METHODS We collected information about the patients and the surgical procedure using a standard form. We included sex, age, and variables with P≤0.2 in the bivariate analysis in a logistic regression model. Additionally, we reviewed the procedures for reprocessing the laparoscopic surgery equipment, and the strains obtained with culture were identified by molecular methods. RESULTS We recorded 60 (27%) cases of infection. After multivariate analysis, the duration of surgery beyond 1 hour (odds ratio [OR] 2.4; 95% confidence interval [CI] 1.2-4.5), not to have been the first operated patient on a given day (OR, 2.7; 95% CI, 1.4-5.2), and the use of permanent trocar (OR, 2.2; 95% CI, 1.1-4.2) were associated with infection. We observed that the surgical team attempted to sterilize the equipment in glutaraldehyde solution when sanitary authorities had already prohibited it. Eleven strains presented 100% DNA identity with a single strain, known as BRA100 clone. CONCLUSIONS Because contaminated material can act as vehicle for infection, ensuring adequate sterilization processing of video-assisted surgery equipment was crucial to stopping this single clonal outbreak of nonturbeculous mycobacteria in Brazil.
Asunto(s)
Brotes de Enfermedades , Desinfección/normas , Laparoscopía/efectos adversos , Infecciones por Mycobacterium no Tuberculosas/epidemiología , Mycobacterium chelonae , Infección de la Herida Quirúrgica/epidemiología , Adulto , Anciano , Anciano de 80 o más Años , Brasil/epidemiología , Infección Hospitalaria/epidemiología , Infección Hospitalaria/microbiología , Infección Hospitalaria/prevención & control , Procedimientos Quirúrgicos del Sistema Digestivo/efectos adversos , Procedimientos Quirúrgicos del Sistema Digestivo/instrumentación , Desinfectantes , Femenino , Glutaral , Adhesión a Directriz , Hospitales Privados , Humanos , Laparoscopía/instrumentación , Masculino , Persona de Mediana Edad , Tipificación Molecular , Infecciones por Mycobacterium no Tuberculosas/microbiología , Infecciones por Mycobacterium no Tuberculosas/prevención & control , Mycobacterium chelonae/clasificación , Mycobacterium chelonae/genética , Estudios Retrospectivos , Factores de Riesgo , Instrumentos Quirúrgicos/efectos adversos , Instrumentos Quirúrgicos/microbiología , Infección de la Herida Quirúrgica/microbiología , Infección de la Herida Quirúrgica/prevención & control , Adulto JovenRESUMEN
Informe técnico da Anvisa sobre diagnóstico e tratamento das infecções por Mycobacterium abcessus. São abordados temas como teste de sensibilidade e coleta e identificação molecular, entre outros.