RESUMEN

Infection with Bovine Viral Diarrhea Viruses (BVDV) in cattle results in a wide range of clinical manifestations, ranging from mild respiratory disease to fetal death and mucosal disease, depending on the virulence of the virus and the immune and reproductive status of the host. In this study 30 Argentinean BVDV isolates were characterized by phylogenetic analysis. The isolates were genotyped based on comparison of the 5' untranslated region (5' UTR) and the E2 gene. In both phylogenetic trees, 76% of the viruses were assigned to BVDV 1b, whereas BVDV 1a, 2a and 2b were also found. Eight of the BVDV 1b isolates were further characterized by cross-neutralization tests using guinea pig antisera and sera from bovines vaccinated with two different commercial vaccines. The results demonstrated the presence of a marked antigenic diversity among Argentinean BVDV isolates and suggest the need to incorporate BVDV 1b isolates in diagnostic strategies.

Asunto(s)

Variación Antigénica/inmunología , Diarrea Mucosa Bovina Viral/inmunología , Virus de la Diarrea Viral Bovina/inmunología , Filogenia , Regiones no Traducidas 5'/genética , Secuencia de Aminoácidos , Animales , Variación Antigénica/genética , Argentina , Secuencia de Bases , Diarrea Mucosa Bovina Viral/genética , Bovinos , Virus de la Diarrea Viral Bovina/genética , Cobayas , Datos de Secuencia Molecular , Pruebas de Neutralización/veterinaria , ARN Viral/química , ARN Viral/genética , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/veterinaria , Alineación de Secuencia , Análisis de Secuencia de ADN , Proteínas del Envoltorio Viral/química , Proteínas del Envoltorio Viral/genética