RESUMEN
Dermal papilla (DP) cells were isolated from rat vibrissae and put into a culture. The homogeneity of the obtained culture was confirmed using immunohistochemical staining with antibodies specific for this type of cell extracellular matrix protein (versican) and staining for alkaline phosphatase. It was demonstrated that the rat vibrissae DP cell culture participates in the development of hair follicles de novo. The ability of the DP culture cells to differentiate in neurons and glia was proved.
Asunto(s)
Diferenciación Celular , Dermis/citología , Folículo Piloso/crecimiento & desarrollo , Neuroglía/citología , Neuronas/citología , Animales , Células Cultivadas , Folículo Piloso/citología , Queratinocitos/citología , Ratones , Ratones Desnudos , Morfogénesis , Ratas , Ratas Sprague-Dawley , Versicanos/análisisRESUMEN
The effect of some growth factors on morphogenetic processes in the primary culture of human epidermal keratinocytes was studied in the model of formation of tubule-like structures in collagen gel. Culturing of keratinocytes in the presence of IGF and bFGF did not induce growth of tubule-like structures, whereas EGF, KGF, and HGF promoted the growth of three-dimensional epidermal outgrowths whose shape and size varied depending on the growth factor used.