RESUMEN

The physical maps the Haemophilus influenzae Sb genomes were constructed by the comparison of restriction fragment sizes of complete single and double digests, as well as by the analysis of partial DNA digests. Sites for four restriction endonucleases NotI, RsrII, SmaI and SrfI were located on the bacterial chromosomes, which are circular, and 2028 and 2045 kb in circumferences. The order of fragments was deduced from fragment patterns of the combinations of double digestion and by two-dimensional Field-Inversion Gel Electrophoresis (FIGE).

Asunto(s)

Cromosomas Bacterianos/genética , Haemophilus influenzae/genética , Mapeo Restrictivo , Secuencia de Bases , ADN Bacteriano/química , ADN Bacteriano/genética , Electroforesis en Gel de Campo Pulsado/métodos , Electroforesis en Gel Bidimensional/métodos , Datos de Secuencia Molecular , Peso Molecular