RESUMEN
Human primers specific for the genes LEP, HBB, PAX3, ESR2, TPH1, ABCA4 and ATP2A2 were used to identify clones in a canine BAC library. Subcloning of the positive BACs in plasmids, screening with microsatellite motifs and subsequent sequencing allowed for the identification of eight novel microsatellites. The presence of the gene of interest was confirmed by sequencing the polymerase chain reaction (PCR) products amplified in the positive BACs. Fluorescent in situ hybridization (FISH) using the positive BACs as probes allowed for the chromosomal localization of the insert DNAs in two canid species, dog (Canis familiaris) and red fox (Vulpes vulpes). The use of gene-associated microsatellites may accelerate the identification of candidate genes for phenotypic traits in linkage studies.
Asunto(s)
Perros/genética , Zorros/genética , Repeticiones de Microsatélite/genética , Animales , Secuencia de Bases , Mapeo Cromosómico , Cromosomas Artificiales Bacterianos , Cartilla de ADN , Genes/genética , Humanos , Hibridación Fluorescente in Situ , Datos de Secuencia Molecular , Análisis de Secuencia de ADNRESUMEN
A 27-year-old flight instructor experienced 5 to 10 minutes after a scuba-dive to 29 m, which lasted totally 50 minutes, dizziness, nausea and severe vertigo. The symptoms lasted about an hour. The patient vomited several times and noted sudden onset headache and vertigo lasting the following three days. Hyperbaric oxygen therapy was started 30 hours after the event because decompression sickness was suspected. Transthoracic echocardiographic evaluation showed a patent foramen ovale. Diving accidents may be caused by decompression sickness, the formation of a free intravascular gas phase (bubbles) may result in transatrial shunting in the presence of a patent foramen ovale and may lead to neurological signs and symptoms. In this context the diver was advised to undergo closure of the atrial septal defect. Five months after the incident the patient underwent successful transcatheter occlusion of the PFO.
Asunto(s)
Enfermedad de Descompresión/diagnóstico , Buceo/efectos adversos , Enfermedad de Meniere/diagnóstico , Vértigo/etiología , Adulto , Enfermedad de Descompresión/etiología , Diagnóstico Diferencial , Humanos , Masculino , Enfermedad de Meniere/etiologíaAsunto(s)
Cromosomas de los Mamíferos/genética , Perros/genética , Monoaminooxidasa/genética , Polimorfismo Genético , Animales , Secuencia de Bases , Cromosomas Artificiales Bacterianos , Cartilla de ADN , Hibridación Fluorescente in Situ , Cariotipificación , Repeticiones de Microsatélite/genética , Datos de Secuencia Molecular , Análisis de Secuencia de ADNRESUMEN
A technique of intracranial facial nerve anastomosis using fenestrated collagen splints was used in eight patients operated upon for acoustic neuroma or glomus temporale tumor. This technique is rapid and simpler than suturing in the cerebellopontine angle. Clinical results are as good as or even better than those obtained with more peripheral facial nerve anastomosis or grafting. An animal study demonstrated no undue histologic reaction to the collagen splints.
Asunto(s)
Neoplasias Cerebelosas/cirugía , Ángulo Pontocerebeloso/cirugía , Nervio Facial/cirugía , Neuroma Acústico/cirugía , Animales , Gatos , Colágeno , Nervio Facial/patología , Humanos , Regeneración Nerviosa , Pronóstico , Férulas (Fijadores) , Nervio Sural/trasplanteRESUMEN
The technique of intraoperative evoked electromyography is described in detail. Direct intraoperative stimulation of the exposed facial nerve identified the sites of nerve impulse conduction blocks in 16 of 18 patients who needed decompression for Bell' s palsy. In order to determine the site of impulse conduction block the test must be performed before 100 per cent nerve degeneration occurs. The lesions extended only a few millimeters and were found to be proximal to the geniculate ganglion in 15 (94 per cent) of these patients. Schirmer's test correctly identified the sites of the lesions in only 61 per cent of the patients. To locate the precise site of the lesion inducing a conduction block in Bell's palsy, intraoperative evoked electromyography must be used. The limited extent of the blocked motor fibers indicates that segmental rather than total intratemporal decompression is needed in Bell's palsy.