RESUMEN
The study comprised the assessment of the effects of secondary amenorrhea on bone tissue metabolism and bone mineral density in girls after forced weight reduction. The study concentrated on assessing and analysing the levels of selected markers of bone tissue remodelling and bone mineral density.
Asunto(s)
Densidad Ósea/fisiología , Huesos/metabolismo , Coerción , Pérdida de Peso , Adolescente , Adulto , Biomarcadores , Femenino , Humanos , Estudios RetrospectivosRESUMEN
The authors described double antibodies radioimmunoassay of human cortisol binding globulin serum level in support of previous isolated and purified trancortin and anti-transcortin antibodies produced by rabbit immunization. Antigen was iodinated by chloraminet method and purification on Ultragel AcA 54 column. Standard curve had specific Bx/Box100%. Using described method investigation of transcortin globulin serum level is precise and repetitive.
Asunto(s)
Proteínas Portadoras/sangre , Segundo Trimestre del Embarazo/sangre , Tercer Trimestre del Embarazo/sangre , Radioinmunoensayo/métodos , Proteínas Portadoras/aislamiento & purificación , Femenino , Humanos , Masculino , Embarazo , Radioinmunoensayo/normas , Valores de Referencia , Reproducibilidad de los Resultados , Sensibilidad y EspecificidadRESUMEN
Authors have presented and assessment of estradiol and progesterone levels in peritoneal fluid and blood serum in women with endometriosis. Peritoneal fluid was collected during laparoscopy performed in luteal phase of the cycle. In this cycle ovulation was controlled in all women. An ovulation was confirmed ultrasonographically and laparoscopically in 45% of women with endometriosis and in 80% of that without the illness. Progesterone concentration in peritoneal fluid in women with endometriosis was significantly lower to the control (p < 0.01).
Asunto(s)
Líquido Ascítico/química , Endometriosis/metabolismo , Estradiol/análisis , Progesterona/análisis , Adulto , Endometriosis/diagnóstico por imagen , Femenino , Humanos , Laparoscopía , Fase Luteínica/fisiología , UltrasonografíaRESUMEN
We used corticosteroid binding globulin purified by chromatography on the Sepharose column for immunisation of the rabbits. We analysed serum by rocket immunoelectrophoresis and cross immunoelectrophoresis. The titre of the serum was estimated by reaction with 125 I-labelled transcortin (corticosteroid binding globulin). The results of our studies indicate that own serum is valuable and can be use for radioimmunoassay of the CBG in the serum.
Asunto(s)
Anticuerpos/sangre , Transcortina/inmunología , Animales , Cromatografía en Agarosa , Femenino , Humanos , Inmunoelectroforesis , Embarazo , Tercer Trimestre del Embarazo , Conejos , RadioinmunoensayoRESUMEN
The isolation of cortisol and progesterone binding globulin (CBG) from pregnant women serum was performed using affinity and hydrophobic chromatography. The purity and specificity of isolated transcortin was tested by agarose gel electrophoresis using racket and cross immunoelectrophoresis and specific CBG antibodies. High purity and immunoreactivity of the isolated globulin destitute of other proteins contamination, were obtained.